Photosystem II-Based Biosensors for Phytoremediation

Giardi, Maria Teresa; Esposito, Dania; Pace, Emanuela; Alessandrelli, S.; Margonelli, Andrea; Angelini, Giancarlo; Giardi, P.

doi:10.1007/978-94-010-0131-1_35

Cited by 2 publications

(3 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Measurement results were found to be consistent with observations recorded from previous experiments performed with the same biomediators and inhibitors by employing a commercial instrument [7]. This comparison strengthens the reliability of the performance of this new multiarray biosensor system.…”

Section: Resultssupporting

confidence: 87%

“…As described in previous works [7], the 'area' parameter has been adopted in fluorescence signal deconvolution for quenching data analysis, due to the strong variations exhibited: it is defined as the area identified by the horizontal line corresponding to the maximum fluorescence value F m and the intensity-time curve response. The output calculated by the onboard electronics, stored in the memory card and represented in the experimental response curves, is the percentage area variation with respect to the initial area value (control, baseline).…”

Section: Experimental Testingmentioning

confidence: 99%

“…In 1931, Kautsky and Hirsch [6] described a typical variation of a plant fluorescence spectrum in response to light exposure, subsequently named the Kautsky effect (also called fluorescence transient, fluorescence induction or fluorescence decay). A bench apparatus for herbicide detection has been previously reported by some of this paper's authors: this instrument allowed chlorophyll fluorescence measurement and Kautsky emission detection of photosynthetic algae immobilized in a single cell, by using a modified commercial plant efficiency analyser from Hansatech Ltd (EU Patent 2006 [7]) [8].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A new miniaturized multiarray biosensor system for fluorescence detection

Tibuzzi

Rea

Pezzotti

et al. 2007

J. Phys.: Condens. Matter

View full text Add to dashboard Cite

A miniaturized biosensor-based optical instrument has been designed and fabricated for multiarray fluorescence measurements of several biomediators in series, with applications in environmental monitoring and agrofood analysis. It is a multicell system featuring two arrays of five static cells (1 × 1 × 2 cm3) which are sealed to avoid contamination. Every cell is made up by two modular sections: the bottom compartment with optical LED light excitations and a photodiode detector for fluorescence emission capture, and the top biocompatible compartment where the biosample is deposited. The system (0.250 kg without batteries and case, 100 × 100 × 150 mm3 internal case dimensions) is equipped with electronic control boards, a flash memory card for automatic data storage, and internal batteries, thus being portable and versatile. The instrument allows one to perform simultaneous and multiparametric analyses and offers a large applicability in biosensor technology. The first prototype has been implemented with genetically modified oxygenic photosynthetic algae that were employed in the instrument experimental testing by monitoring pesticide pollution in water. Pesticides modify the photosystem II (PSII) activity in terms of fluorescence quenching. The PSII complex features a natural nanostructure and can be considered a sophisticated molecular device. Results from measurements employing several PSII mutants and six different pesticides at increasing concentrations and incubation times are presented and discussed.

show abstract

Section: Resultssupporting

confidence: 87%

Section: Experimental Testingmentioning

confidence: 99%