Photosystem II Damage and Repair Cycle in the Green Alga Dunaliella salina: Involvement of a Chloroplast-Localized HSP70

Yokthongwattana, Kittisak; Chrost, Bozena; Behrman, Shannon; Casper-Lindley, Catharina; Melis, Anastasios

doi:10.1093/pcp/pce179

Cited by 71 publications

(56 citation statements)

References 49 publications

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“…This hypothesis is consistent with the observation that V de-epoxidation occurs immediately upon photodamage, and Z accumulation occurs in parallel with the accumulation of photodamaged PSII reaction centers in the chloroplast thylakoids. A prompt disassembly of the PSII holocomplex (Melis, 1991;Aro et al, 1993) and formation of a PSII repair intermediate (Melis, 1999;Yokthongwattana et al, 2001) are known to follow photodamage. Highly photoactive tetrapyrrole pigments are released by the PSII D1/D2 reaction center in the course of reaction center disassembly and D1 degradation and replacement.…”

Section: Discussionmentioning

confidence: 99%

“…SDS-PAGE analysis of the thylakoid membrane proteins was performed with samples loaded on an equal Chl basis, followed by western-blot analyses with specific polyclonal antibodies. Figure 3 shows western blots with specific polyclonal antibodies against the D1 reaction center protein of PSII (Kim et al, 1993) or against a 160-kD PSII repair intermediate (Kim et al, 1993;Melis and Nemson, 1995;Yokthongwattana et al, 2001). The 160-kD protein complex is known to contain a photodamaged but as yet undegraded D1 protein (Kim et al, 1993) and the D2 protein of the PSII reaction center (Melis and Nemson, 1995).…”

Section: Photo-inhibition Status As a Function Of Growth Irradiancementioning

confidence: 99%

“…This unusual property has permitted, for the first time to our knowledge, an SDS-PAGE-based quantitation of photodamaged versus active D1 in chloroplast thylakoids (Baroli and Melis, 1996). It was postulated that formation of such a 160-kD protein complex might reflect PSII conformational changes that occur as a direct consequence of photodamage and the ensuing partial disassembly of PSII (Yokthongwattana et al, 2001). The quantitative measurement of the 160-kD protein complex provides a convenient way by which to assess the extent of the in vivo photo-inhibition in D. salina.…”

Section: Photo-inhibition Status As a Function Of Growth Irradiancementioning

confidence: 99%

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Role of the Reversible Xanthophyll Cycle in the Photosystem II Damage and Repair Cycle in Dunaliella salina

et al. 2003

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The Dunaliella salina photosynthetic apparatus organization and function was investigated in wild type (WT) and a mutant (zea1) lacking all ␤,␤-epoxycarotenoids derived from zeaxanthin (Z). The zea1 mutant lacked antheraxanthin, violaxanthin, and neoxanthin from its thylakoid membranes but constitutively accumulated Z instead. It also lacked the so-called xanthophyll cycle, which, upon irradiance stress, reversibly converts violaxanthin to Z via a de-epoxidation reaction. Despite the pronounced difference observed in the composition of ␤,␤-epoxycarotenoids between WT and zea1, no discernible difference could be observed between the two strains in terms of growth, photosynthesis, organization of the photosynthetic apparatus, photo-acclimation, sensitivity to photodamage, or recovery from photo-inhibition. WT and zea1 were probed for the above parameters over a broad range of growth irradiance and upon light shift experiments (low light to high light shift and vice versa). A constitutive accumulation of Z in the zea1 strain did not affect the acclimation of the photosynthetic apparatus to irradiance, as evidenced by indistinguishable irradiance-dependent adjustments in the chlorophyll antenna size and photosystem content of WT and zea1 strain. In addition, a constitutive accumulation of Z in the zea1 strain did not affect rates of photodamage or the recovery of the photosynthetic apparatus from photo-inhibition. However, Z in the WT accumulated in parallel with the accumulation of photodamaged PSII centers in the chloroplast thylakoids and decayed in tandem with a chloroplast recovery from photo-inhibition. These results suggest a role for Z in the protection of photodamaged and disassembled PSII reaction centers, apparently needed while PSII is in the process of degradation and replacement of the D1/32-kD reaction center protein.Organisms of oxygenic photosynthesis convert the energy of sunlight into chemical energy, which supports most life on earth. In photosynthetic membranes of green algae and plants, incident irradiance is absorbed by chlorophyll (Chl)-binding lightharvesting antenna complexes (LHCs) associated with the reaction centers of PSII and PSI. However, when the photosynthetic apparatus absorbs irradiance in excess of that required for the saturation of photosynthesis, singlet oxygen is generated, and PSII is subject to an irreversible photooxidative damage (Vass et al., 1992; Telfer et al., 1994; Melis, 1999). This photodamage selectively impairs the function of the D1/32-kD reaction center protein of PSII and has the potential to lower rates of photosynthesis and diminish plant growth and productivity (Powles and Critchley, 1980; Powles, 1984). The probability of photooxidative damage in chloroplasts depends on the oxidation reduction state of the primary electron-accepting plastoquinone of PSII (Q A ), which is the parameter that controls photodamage under a variety of physiological and environmental conditions. When Q A is oxidized under continuous illumination, photochemical electron transport...

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Section: Discussionmentioning

confidence: 99%

Section: Photo-inhibition Status As a Function Of Growth Irradiancementioning

confidence: 99%

Section: Photo-inhibition Status As a Function Of Growth Irradiancementioning

confidence: 99%

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Role of the Reversible Xanthophyll Cycle in the Photosystem II Damage and Repair Cycle in Dunaliella salina

et al. 2003

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“…It has, however, been suggested that a kind of chloroplast Hsp70 in Dunaliella salina plays some role in repairing damaged D1 or in stabilizing dissociated components of the PSII complex. 16) Therefore, Hsp70 in chloroplast or cyanobacteria might have specific functions in the photosynthetic machinery.…”

Section: Discussionmentioning

confidence: 99%

Involvement of DnaK3, One of the Three DnaK Proteins of CyanobacteriumSynechococcussp. PCC7942, in Translational Process on the Surface of the Thylakoid Membrane

Katano

Nimura-Matsune

Yoshikawa

2006

Bioscience, Biotechnology, and Biochemistry

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“…after injury by UV radiation) by reducing the inactivation of PSII and intensifying the recovery. The role of the stress proteins in the repair of the photodamaged PSII would be the stabilization of the core of PSII reaction centres (Yokthongwattana et al, 2001). In macroalgae, exposure to UV radiation in the laboratory has proved to be a factor inducing HSP70, which depends on the UV dose and species.…”

Section: Uv Radiationmentioning

confidence: 99%

Stress proteins and auxiliary anti stress compounds in intertidal macroalgae

Cruces

Huovinen

Gómez

2012

lajar

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ABSTRACT. Intertidal macroalgae are exposed to strong variation in the physical environment and thus, diverse anti-stress mechanisms are displayed by these organisms. Stress proteins (also called heat shock proteins, HSPs) have been invoked as potential protective mechanism, especially during stressful action of temperature and solar radiation. Therefore, macroalgae have not normally been used as model organisms in studies of these molecules. The present study compiles the existing information from intertidal species in the context of major factors that have been reported to induce them, e.g. temperature, enhanced solar radiation, contaminants, etc. Additionally, in order to address the question whether the expression of these proteins operates in intertidal macroalgae complementarily with other protective mechanisms, a case study of induction of HSPs after exposure to UV radiation and high temperature in two upper littoral species, Ulva sp. and Porphyra columbina, from southern Chile is presented. In parallel, two well-known responses to stress, photoinhibition of photochemical reactions (F v /F m ) and ROS scavenging were measured. The results indicated that, although stress proteins were detected in a time span between 3 and 24 h, the responses were not correlated with photochemical and antioxidative response. Overall, the study outlines a potential role of stress proteins in ecophysiological responses developed to cope mainly with high temperature and UV radiation. However, other rapid metabolic adjustments (e.g. high thermo-tolerance of photosynthesis and efficient ROS scavenging), together with other biomolecules (mycosporines, phenols, polyamines, etc.) and morphofunctional adaptations to the intertidal life (e.g. small size, high area/volume ratio) are also important. Keywords: stress proteins, intertidal macroalgae, photosynthesis, ROS, UV radiation, photoprotection. Proteínas de estrés y compuestos anti-estrés auxiliares en algas marinas intermarealesRESUMEN. Las macroalgas marinas intermareales están expuestas a extrema variación en las condiciones ambientales y por ello desarrollan una serie de mecanismos anti-estrés. Las proteínas de estrés (HSPs) han sido consideradas como potenciales agentes protectores en respuesta a condiciones estresantes, especialmente durante la acción de elevada temperatura y alta radiación solar. Considerando que las macroalgas marinas no han sido usadas comúnmente como modelos de estudio para analizar estas moléculas, el presente trabajo compila la información existente sobre la inducción de proteínas de estrés en algas intermareales en el contexto de los principales factores hasta ahora reportados como inductores, e.g. temperatura, alta radiación solar, contaminantes, etc. Adicionalmente, mediante un estudio de caso usando dos especies del intermareal superior, Ulva sp. y Porphyra columbina colectadas en el sur de Chile, se examina si la inducción de proteínas de estrés ocurre de forma complementaria con otras respuestas anti-stress. Para ello, dos mecanismos bien conocid...

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Photosystem II Damage and Repair Cycle in the Green Alga Dunaliella salina: Involvement of a Chloroplast-Localized HSP70

Cited by 71 publications

References 49 publications

Role of the Reversible Xanthophyll Cycle in the Photosystem II Damage and Repair Cycle in Dunaliella salina

Role of the Reversible Xanthophyll Cycle in the Photosystem II Damage and Repair Cycle in Dunaliella salina

Involvement of DnaK3, One of the Three DnaK Proteins of CyanobacteriumSynechococcussp. PCC7942, in Translational Process on the Surface of the Thylakoid Membrane

Stress proteins and auxiliary anti stress compounds in intertidal macroalgae

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