Phototherapy promotes healing of chronic diabetic leg ulcers that failed to respond to other therapies

Minatel, Débora Garbin; Frade, Marco Andrey Cipriani; França, Suzelei C.; Enwemeka, Chukuka S.

doi:10.1002/lsm.20789

Cited by 146 publications

(127 citation statements)

References 78 publications

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“…In addition to increased MMP-9, other studies have also found increased levels of MMP-2 and -8 [4][5][6]. PBM has been shown to stimulate cells and wound healing, and has aided in the healing of chronic diabetic ulcers [8][9][10][11][12][13]. Aparecida Da Silva and co-workers irradiated diabetic induced male Wistar rats (660 nm, 4 J/cm2) and evaluated MMP-2 and -9, and Type I and III collagen 24 h post-irradiation [25].…”

Section: Resultsmentioning

confidence: 99%

“…This leads to an increase in adenosine triphosphate (ATP) which in turn activates a cascade of intracellular signals and messengers, leading to various down-stream effects such as increased proliferation, cellular migration and viability, collagen production, as well as gene transcription [8]. There are several research papers which demonstrate the beneficial effect of PBM in the treatment of diabetic ulcers [9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

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Influence of 660 and 830 nm Laser Irradiation on Genetic Profile of Extracellular Matrix Proteins in Diabetic Wounded Human Skin Fibroblast Cells

Houreld

2016

Proceedings of the 2016 International Conference on Biomedical and Biological Engineering

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Abstract. The extracellular matrix (ECM) provides tissue structural integrity and its synthesis plays a crucial role in wound healing. Impaired wound healing ensues following the destruction of the ECM, or the inhibition of its synthesis. An imbalance in ECM synthesis and degradation is seen in patients with diabetes. This has led to the development of novel therapies that aim to decrease ECM destruction and increase its synthesis. Photobiomodulation (PBM) has been shown to speed up the healing of these slow-to-heal wounds, and numerous studies are being conducted to determine the underlying molecular cause. This study aimed to ascertain the effect of laser irradiation at a wavelength of 660 or 830 nm at a dose of 5 J/cm 2 on the genetic expression profile of genes involved in ECM proteins. cDNA was reverse transcribed from total isolated RNA and used in real-time qualitative polymerase chain reaction (qPCR). Genes concerned with the basement membrane, collagen and ECM structural constituents, and ECM protesases and inhibitors were evaluated. Results showed that a similar genetic profile (although not identical) was seen post irradiation. PBM was able to reduce the expression of ECM proteases and increase mRNA levels of ECM proteins. This study fortifies the notion that PBM stimulates cellular activity and can influence ECM matrix synthesis and degradation during wound healing. This study has also shown that PBM is able to stimulate cells at a genetic level.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Influence of 660 and 830 nm Laser Irradiation on Genetic Profile of Extracellular Matrix Proteins in Diabetic Wounded Human Skin Fibroblast Cells

Houreld

2016

Proceedings of the 2016 International Conference on Biomedical and Biological Engineering

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“…In addition there is evidence that such techniques may be effective in treating a range of medical conditions including diabetic leg ulcers [159,160], pancreatic cancer [161], etc.…”

Section: Light and Colour As A Therapeutic Modalitymentioning

confidence: 99%

“…Accordingly it is completely unrealistic to expect that the results from clinical studies, based upon an incomplete understanding of the phenomena, can give an optimum physiological response. Nevertheless light-based biofeedback, neurofeedback and laser-based techniques have become widely used for the treatment of a range of conditions including psoriasis, seasonal-affective disorder, hyperbilirubinaemia, dyslexia.In addition there is evidence that such techniques may be effective in treating a range of medical conditions including diabetic leg ulcers [159,160], pancreatic cancer [161], etc.It has taken a great deal of time for the person to become unwell. Accordingly it is unreasonable to expect instantaneous relief.…”

mentioning

confidence: 99%

The Framework of a Mathematical Model of the Autonomic Nervous System and Physiological Systems: Using the Neuroregulation of Blood Glucose as an Example

Ewing¹

2015

J Comput Sci Syst Biol

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IntroductionOur sense perception enables us to eat the correct amounts of food and, under normal circumstances, we cease eating when we have eaten sufficient food, and we drink sufficient to sati sfy the body 's need for liquid. We breathe at the normal expected rate, our heart pumps at the expected rate and maintain the normal level of blood pressure, our body maintains the normal levels of acidity and temperature, we sleep for 7-8 hours per night, etc. This suggests that the body is being regulated by a biological control mechanism of enormous sophistication and complexity, which has an enormous processing capacity; however as we age, and particularly as we age beyond 50 years, the mechanism which maintains our function become increasingly less able to sustain the levels of oxygen, blood glucose, blood cell content and a myriad of other biological components which the brain requires to function [1]. The i nnate g enetic c apacity o f key o rgans d eclines a nd r esults in shortages of proteins, hormones, etc. This h as a k nock-on e ffect affecting one biological sequence after another.The cumulative effect of our lifetime of experiences, due to environmental and biological factors, influences the function and/ or stability of the autonomic nervous system and ultimately the function of our DNA and genes [2]. The level of genetic expression of the proteins (genotype) which we require to sustain our function declines and the rate at which these expressed proteins subsequently react (phenotype) also declines i.e. the ability of the body to cope with extreme events declines.There are two fundamentally significant biological issues at play: (i) the genetic ability to express proteins (ii) the body's physiological demand i.e. the phenotype or influence of the environment. In the case of diabetes, in particular type 2 diabetes, this is the regulation of Blood Glucose. The greater the level of hyperglycaemia the greater is the associated demand for insulin. The development of a medical condition occurs when the environmental demand exceeds the body's innate capacity [3] i.e. the prevailing supply and/or level of insulin is insufficient to metabolise the excess of blood glucose. By contrast, the lower the level of hypoglycaemia, which occurs in type 1 diabetes, the lower is the demand/need for insulin. If there is insufficient blood glucose the excess of highly reactive insulin will seek an alternative substrate and ultimately results in damage to the peripheral blood vessels e.g. in particular to the brain, eyes, legs and feet.The evidence suggests that this regulatory process is performed by the brain via the process of neuroregulation [4][5][6]. This is a best-fit process which takes into account the prevailing state of development or physiological damage to a part of the brain, degeneration or damage to an organ (or limb), and the prevailing inter-cellular environment i.e. the feedback of biochemical signals from the viscera. AbstractDespite the immense advances of medical research there remains a fundamental theo...

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“…Later, the ima ges were analy zed using the color decon vo lu tion plu gin func tion of the ImageJ soft wa re (US National Institutes of Health, Bethesda, MD, USA), which supplies the per cen ta ge of blue stai ning (col la gen) in each ROI. [18][19][20][21][22] …”

Section: Analysis Of Col La Ge Ne Sismentioning

confidence: 99%

Modelos experimentais de desnutrição e sua influência no trofismo cutâneo

Leite

Júnior

Andrade

et al. 2011

An. Bras. Dermatol.

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BACKGROUND: The skin requires adequate levels of nutrients to function properly. Objective: To analyze skin trophism in well-nourished and undernourished rats using two models of malnutrition. METHODS: In the marasmus model, 60 Wistar rats were kept on a controlled diet, 30 being randomly selected to receive half the established diet for 60 days. In the gelatin model, 60 rats were used, 30 of which received a diet consisting of poor quality protein (gelatin) for 30 days. The nutritional status of the animals was evaluated according to body mass index, clinical signs and serum albumin measurement. After the period of malnutrition, histology was performed on the animals' skin to analyze the thickness of the dermis and epidermis using the Leica Application Suite software. Collagen was analyzed on slides stained with Gömöri trichrome using the ImageJ software program. RESULTS: The body mass index of the malnourished animals in the marasmus and gelatin groups was significantly lower than that of the well-nourished animals in the two groups (p<0.0001 in both models). With respect to serum albumin, there was no difference between the groups in either of the two models. In relation to the histological analysis of skin thickness, the dermis of the malnourished animals was significantly thinner compared to that of the well-nourished animals (p<0.0001 in both models). The percentage of collagen was lower in the malnourished animals compared to the well-nourished animals (p<0.0005 and p<0.003 in the marasmus and gelatin model, respectively). CONCLUSIONS: Skin thickness measurements were lower in the malnourished animals in both models, and this finding was histologically confirmed by the lower percentage of collagen, showing the negative effect of malnutrition on skin trophism. Keywords: Collagen; gelatin; image processing, computer-assisted; nutritional marasmus; public health; skin.Resumo: FUNDAMENTOS: A pele, para exercer suas funções, necessita de níveis adequados de nutrientes. OBJETIVO: Analisar o trofismo cutâneo de ratos nutridos e desnutridos por meio de dois modelos de desnutrição. MÉTODOS: No Modelo Marasmo, utilizaram-se 60 ratos Wistar em controle dietético, dos quais 30 foram selecionados aleatoriamente para receber metade da dieta diária durante 60 dias. No Modelo Gelatina, empregaram-se 60 ratos, dos quais 30 receberam dieta associada a proteína de baixa qualidade (gelatina) durante 30 dias. Avaliou-se o estado nutricional dos animais por meio da massa corporal, dos sinais clínicos e da dosagem de albumina sérica. Após o período de desnutrição, fez-se a histologia da pele dos animais para análise da espessura da derme e epiderme com o software Leica Application Suite; nas lâminas coradas com tricrômio de Gomori, analisou-se a colagênese com o software ImageJ. RESULTADOS: A massa corporal dos animais desnutridos pelo marasmo e gelatina foi significativamente menor (p<0,0001 e p<0,0001) do que a dos grupos nutridos. Quanto à albumina sérica, não houve diferença entre os grupos nos dois modelos. Em re...

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Phototherapy promotes healing of chronic diabetic leg ulcers that failed to respond to other therapies

Abstract: Combined 660 and 890 nm light promotes rapid granulation and healing of diabetic ulcers that failed to respond to other forms of treatment.

Cited by 146 publications

References 78 publications

Influence of 660 and 830 nm Laser Irradiation on Genetic Profile of Extracellular Matrix Proteins in Diabetic Wounded Human Skin Fibroblast Cells

Influence of 660 and 830 nm Laser Irradiation on Genetic Profile of Extracellular Matrix Proteins in Diabetic Wounded Human Skin Fibroblast Cells

The Framework of a Mathematical Model of the Autonomic Nervous System and Physiological Systems: Using the Neuroregulation of Blood Glucose as an Example

Modelos experimentais de desnutrição e sua influência no trofismo cutâneo

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