Photothermal excitation and laser Doppler velocimetry of higher cantilever vibration modes for dynamic atomic force microscopy in liquid

Nishida, Shuhei; Kobayashi, D.; Sakurada, Takeo; Nakazawa, Takashi; Hoshi, Yasuo; Kawakatsu, Hideki

doi:10.1063/1.3040500

Cited by 37 publications

(26 citation statements)

References 24 publications

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“…Hoogenboom et al presented an atomic-scale image of mica obtained in water and molecular-resolution images of bacteriorhodopsins in buffer solution [47] using a low-noise interferometer [48]. Kawakatsu and co-workers also obtained an atomic-scale images of mica in water [49] using a low-noise Doppler interferometer [50].…”

Section: Historical Backgroundmentioning

confidence: 99%

Water distribution at solid/liquid interfaces visualized by frequency modulation atomic force microscopy

Fukuma

2010

Science and Technology of Advanced Materials

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Interfacial phenomena at solid/water interfaces play an important role in a wide range of industrial technologies and biological processes. However, it has been a great challenge to directly probe the molecular-scale behavior of water at solid/water interfaces. Recently, there have been tremendous advancements in frequency modulation atomic force microscopy (FM-AFM), enabling its operation in liquids with atomic resolution. The high spatial and force resolutions of FM-AFM have enabled the visualization of one-dimensional (1D) profiles of the hydration force, two-dimensional (2D) images of hydration layers and three-dimensional (3D) images of the water distribution at solid/water interfaces. Here I present an overview of the recent advances in FM-AFM instrumentation and its applications to the study of solid/water interfaces.

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Section: Historical Backgroundmentioning

confidence: 99%

Water distribution at solid/liquid interfaces visualized by frequency modulation atomic force microscopy

Fukuma

2010

Science and Technology of Advanced Materials

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“…Examples of the early applications include minerals [12,[14][15][16] and biological systems [14,[17][18][19][20][21][22]. While these results highlighted the high spatial resolution and wide applicability of liquid-environment FM-AFM, they also clarified its fundamental limitation.…”

Section: Introductionmentioning

confidence: 89%

Advanced Instrumentation of Frequency Modulation AFM for Subnanometer-Scale 2D/3D Measurements at Solid-Liquid Interfaces

Fukuma

2015

Noncontact Atomic Force Microscopy

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Since the first demonstration of true atomic-resolution imaging by frequency modulation atomic force microscopy (FM-AFM) in liquid, the method has been used for imaging subnanometer-scale structures of various materials including minerals, biological systems and other organic molecules. Rencetly, there have been further advancements in the FM-AFM instrumentation. Three-dimensional (3D) force measurement techniques are proposed for visualizing 3D hydration structures formed at a solid-liquid interface. These methods further enabled to visualize 3D distributions of flexible surface structures at interfaces between soft materials and water. Furthermore, the fundamental performance such as force sensitivity and operation speed have been significantly improved using a small cantilever and highspeed phase detector. These technical advancements enabled direct visualization of atomic-scale interfacial phenomena at 1 frame/sec. In this chapter, these recent advancements in the FM-AFM instrumentation and their applications to the studies on various interfacial phenomena are presented.

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“…The signal was recorded with a sampling rate of 10 Hz, and a frequency drift of the system was below 100 Hz. 26,27 The liquid was introduced by a push/pull syringe pump (KD scientific, KD270) connected to both sides of the microchannel in order to stabilize the liquid pressure on the cantilever. In addition, the new bio-solution was loaded in an injector placed between the pump and the inlet of the microchannel (Fig.…”

Section: Cantilever Resonating At the Air-liquid Interfacementioning

confidence: 99%

Application of a new microcantilever biosensor resonating at the air–liquid interface for direct insulin detection and continuous monitoring of enzymatic reactions

et al. 2012

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Here we describe the application of a recently developed high-resolution microcantilever biosensor resonating at the air-liquid interface for the continuous detection of antigen-antibody and enzymesubstrate interactions. The cantilever at the air-liquid interface demonstrated 50% higher quality factor and a 5.7-fold increase in signal-to-noise-ratio (SNR) compared with one immersed in the purified water. First, a label-free detection of a low molecular weight protein (insulin, 5.8 kDa) in physiological concentration was demonstrated. The liquid facing side of the cantilever was functionalized by coating its surface with insulin antibodies, while the opposite side was exposed to air. The meniscus membrane at the micro-slit around the cantilever sustained the liquid in the microchannel. After optimizing the process of surface functionalization, the resonance frequency shift was successfully measured for insulin solutions of 0.4, 2.0, and 6.3 ng ml 21. To demonstrate additional application of the device for monitoring enzymatic protein degradation, the liquid facing microcantilever surface was coated with human recombinant SOD1 (superoxide dismutase 1) and exposed to various concentrations of proteinase K solution, and the kinetics of the SOD1 digestion was continuously monitored. The results showed that it is a suitable tool for sensitive protein detection and analysis.

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Photothermal excitation and laser Doppler velocimetry of higher cantilever vibration modes for dynamic atomic force microscopy in liquid

Cited by 37 publications

References 24 publications

Water distribution at solid/liquid interfaces visualized by frequency modulation atomic force microscopy

Water distribution at solid/liquid interfaces visualized by frequency modulation atomic force microscopy

Advanced Instrumentation of Frequency Modulation AFM for Subnanometer-Scale 2D/3D Measurements at Solid-Liquid Interfaces

Application of a new microcantilever biosensor resonating at the air–liquid interface for direct insulin detection and continuous monitoring of enzymatic reactions

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