Photothermal-Induced Antibacterial Activity of Gold Nanorods Loaded into Polymeric Hydrogel against Pseudomonas aeruginosa Biofilm

Abstract

In this study, the photothermal-induced bactericidal activity of phospholipid-decorated gold nanorods (DSPE-AuNR) suspension against Pseudomonas aeruginosa planktonic and biofilm cultures was investigated. We found that the treatment of planktonic culture of Pseudomonas aeruginosa with DSPE-AuNR suspension (0.25–0.03 nM) followed by a continuous laser beam exposure resulted in ~6 log cycle reduction of the bacterial viable count in comparison to the control. The percentage reduction of Pseudomonas aeruginosa biofilm viable count was ~2.5–6.0 log cycle upon laser excitation with different concentrations of DSPE-AuNR as compared to the control. The photothermal ablation activity of DSPE-AuNR (0.125 nM) loaded into poloxamer 407 hydrogel against Pseudomonas aeruginosa biofilm resulted in ~4.5–5 log cycle reduction in the biofilm viable count compared to the control. Moreover, transmission electron microscope (TEM) images of the photothermally-treated bacteria revealed a significant change in the bacterial shape and lysis of the bacterial cell membrane in comparison to the untreated bacteria. Furthermore, the results revealed that continuous and pulse laser beam modes effected a comparable photothermal-induced bactericidal activity. Therefore, it can be concluded that phospholipid-coated gold nanorods present a promising nanoplatform to eradicate Pseudomonas aeruginosa biofilm responsible for common skin diseases.

Фототермическое действие инфракрасного (808 nm) лазерного излучения и наночастиц золота в различных модификациях на S. aureus

“…В тех случаях, когда контакт клеточной стенки и НЧ влечет за собой необратимые разрушения (действие активных форм кислорода (АФК), повреждения при миграции энергии при плазмонном резонансе), более выраженный эффект достигается наличием развитой поверхности [41][42][43]. При фотодинамической или фототермической терапии с использованием инфракрасного лазерного излучения предпочтительно использовать НЧ с настраиваемым плазмонным резонансом в окне прозрачности тканей при 750−900 nm, и первым выбором в этом случае являются наностержни [44,45]. В нашем случае именно настройка плазмонного резонанса на длину волны излучения лазера сыграла свою роль в более эффективном действии наностержней по сравнению с нанокубиками (рис.…”

Photothermally active nanoparticles as a promising tool for eliminating bacteria and biofilms

“…The authors found that the fabricated nanocomposite showed pronounced antimicrobial activity against tested pathogenic microorganisms with limited cytotoxicity. In another recent publication, the photothermal effect of phospholipid-coated gold nanorods loaded into a poloxamer 407 hydrogel resulted in ≈4.5-5 log cycle reduction in the viable counts of a P. aeruginosa biofilm in comparison to the control sample [55]. The authors did not discern a difference in bacteria eradication with respect to the laser irradiation modality (continuous or pulsed).…”

“…The gold nanoparticle aggregation induced a change in the observed LSPR band, increasing both the NIR absorption and the photothermal conversion, which allowed for the elimination of Gram-positive bacteria. In another recent work, the photothermal-triggered effect of phospholipid-coated gold nanorods against P. aeruginosa planktonic and biofilm cultures was studied [55]. The authors found that the interaction between a planktonic culture of P. aeruginosa and a nanoparticle suspension, under NIR light exposure, resulted in a ≈6 log cycle reduction in the bacterial viable count with respect to the control.…”

Graphene Oxide Mediated Broad-Spectrum Antibacterial Based on Bimodal Action of Photodynamic and Photothermal Effects

“…The necrotic cell death stimulates inflammatory responses which may usually compromise the antitumor activity (Pérez-Hernández et al, 2015). PTT is used for both tumor removal (Yang et al, 2010;Robinson et al, 2011;Tu et al, 2014;Wang et al, 2015) and bacterial disinfection in various types of bacteria Al-Bakri and Mahmoud, 2019;Qing et al, 2019).…”