Photothermal Therapy as Adjuvant to Surgery in an Orthotopic Mouse Model of Human Fibrosarcoma

Simón, Marina; Jørgensen, Jesper Tranekjær; Melander, Fredrik; Andresen, Thomas Lars; Christensen, Anders; Kjær, Andreas

doi:10.3390/cancers13225820

Cited by 11 publications

(7 citation statements)

References 34 publications

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“…All mice treated with NS-based PTT (PRRT + PTT day 1, PRRT + PTT day 6, and PTT groups) reached very high surface temperatures after five minutes of irradiation (overall mean of 48.8 ± 0.2 • C), detected with a thermal camera (Figure 3B,C). These temperatures were comparable to what has previously been reported for NS-based PTT in other tumor models [33,36,37], and significantly higher (p < 0.001) than the ones reached on the tumors of the animals that were not injected with NS but were still treated with the laser (PRRT + laser day 1 and PRRT + laser day 6 groups, mean of 42.3 ± 0.2 • C). For the groups receiving PRRT, the [ 177 Lu]Lu-DOTA-TATE injection was done on day 0.…”

Section: Uptake and Intratumoral Distribution Of [64cu]cu-dota-tate I...supporting

confidence: 88%

“…This is primarily due to the limited light penetration depth of the NIR laser and heterogeneous accumulation of the photoabsorber in the tumor [21,22,27]. However, PTT has shown promise as an add-on to chemotherapy, radiotherapy, and surgery by improving drug accumulation, sensitizing cells to ionizing radiation, and removing tumor remnants after incomplete surgical resection [28][29][30][31][32][33]. Likewise, PTT could potentially improve treatment outcome when applied in combination with PRRT.…”

Section: Introductionmentioning

confidence: 99%

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Combination of [177Lu]Lu-DOTA-TATE Targeted Radionuclide Therapy and Photothermal Therapy as a Promising Approach for Cancer Treatment: In Vivo Studies in a Human Xenograft Mouse Model

et al. 2022

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Peptide receptor radionuclide therapy (PRRT) relies on α- and β-emitting radionuclides bound to a peptide that commonly targets somatostatin receptors (SSTRs) for the localized killing of tumors through ionizing radiation. A Lutetium-177 (177Lu)-based probe linked to the somatostatin analog octreotate ([177Lu]Lu-DOTA-TATE) is approved for the treatment of certain SSTR-expressing tumors and has been shown to improve survival. However, a limiting factor of PRRT is the potential toxicity derived from the high doses needed to kill the tumor. This could be circumvented by combining PRRT with other treatments for an enhanced anti-tumor effect. Photothermal therapy (PTT) relies on nanoparticle-induced hyperthermia for cancer treatment and could be a useful add-on to PRRT. Here, we investigate a strategy combining [177Lu]Lu-DOTA-TATE PRRT and nanoshell (NS)-based PTT for the treatment of SSTR-expressing small-cell lung tumors in mice. Our results showed that the combination treatment improved survival compared to PRRT alone, but only when PTT was performed one day after [177Lu]Lu-DOTA-TATE injection (one of the timepoints examined), showcasing the effect of treatment timing in relation to outcome. Furthermore, the combination treatment was well-tolerated in the mice. This indicates that strategies involving NS-based PTT as an add-on to PRRT could be promising and should be investigated further.

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Section: Uptake and Intratumoral Distribution Of [64cu]cu-dota-tate I...supporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Combination of [177Lu]Lu-DOTA-TATE Targeted Radionuclide Therapy and Photothermal Therapy as a Promising Approach for Cancer Treatment: In Vivo Studies in a Human Xenograft Mouse Model

et al. 2022

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“…), in-depth optimization of the treatment protocol would be needed before clinical translation. 10 , 32 , 48 , 49 …”

Section: Discussionmentioning

confidence: 99%

Neoadjuvant Gold Nanoshell-Based Photothermal Therapy Combined with Liposomal Doxorubicin in a Mouse Model of Colorectal Cancer

Simón¹,

Jørgensen²,

Nørregaard³

et al. 2023

IJN

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Introduction Traditional cancer treatments, such as chemotherapy, are often incapable of achieving complete responses as standalone therapies. Hence, current treatment strategies typically rely on a combination of several approaches. Nanoparticle-based photothermal therapy (PTT) is a technique used to kill cancer cells through localized, severe hyperthermia that has shown promise as an add-on treatment to multiple cancer therapies. Here, we evaluated whether the combination of gold nanoshell (NS)-based PTT and liposomal doxorubicin could improve outcome in a mouse model of colorectal cancer. Methods First, NS-based PTT was performed on tumor-bearing mice. Radiolabeled liposomes were then injected at different timepoints to follow their accumulation in the tumor and determine the ideal injection time after PTT. In addition, fluorescent liposomes were used to observe the liposomal distribution in the tumor after PTT. Finally, we combined PTT and doxorubicin-loaded liposomes and studied the effect of the treatment strategy on the mice by following tumor growth and survival. Results PTT significantly improved liposomal accumulation in the tumor, but only when the liposomes were injected immediately after the therapy. The liposomes accumulated mostly in regions adjacent to the ablated areas. When PTT was combined with liposomal doxorubicin, the mice experienced a slowdown in tumor growth and an improvement in survival. Conclusion According to our preclinical study, NS-based PTT seems promising as an add-on treatment for liposomal chemotherapy and potentially other systemic therapies, and could be relevant for future application in a clinical setting.

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“…The tumor volume was calculated according to the formula volume = length × width 2 × 0.5, and an MR examination was performed when the tumor volume reached 800–1000 mm 3. 15 …”

Section: Methodsmentioning

confidence: 99%

Correlation of Apparent Diffusion Coefficient With Proliferation and Apoptotic Indexes in a Murine Model of Fibrosarcoma: Comparison of Four Methods for MRI Region of Interest Positioning

Yang

Fang

Tao

et al. 2022

Magnetic Resonance Imaging

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Background: Diffusion-weighted imaging (DWI) has demonstrated great potential in predicting the expression of tumor cell proliferation and apoptosis indexes. Purpose: To evaluate the impact of four region of interest (ROI) methods on interobserver variability and apparent diffusion coefficient (ADC) values and to examine the correlation of ADC values with Ki-67, Bcl-2, and P53 labeling indexes (LIs) in a murine model of fibrosarcoma. Study Type: Prospective, animal model. Animal Model: A total of 22 female BALB/c mice bearing intramuscular fibrosarcoma xenografts. Field Strength/Sequence: A 3.0 T/T1-weighted fast spin-echo (FSE), T2-weighted fast relaxation fast spin-echo, and DWI PROPELLER FSE sequences. Assessment: Four radiologists measured ADC values using four ROI methods (oval, freehand, small-sample, and wholevolume). Immunohistochemical assessment of Ki-67, Bcl-2, and P53 LIs was performed. Statistical Tests: Interclass correlation coefficient (ICC), one-way analysis of variance followed by LSD-t post hoc analysis, and Pearson correlation test were performed. The statistical threshold was defined as a P-value of <0.05. Results: All ROI methods for ADC measurements showed excellent interobserver agreement (ICC range, 0.832-0.986). The ADC values demonstrated significant differences among the four ROI methods. The ADC values for oval, freehand, small-sample, and whole-volume ROI methods showed a moderately negative correlation with Ki-67 (r = À0.623; r = À0.629; r = À0.642, and r = À0.431) and Bcl-2 (r = À0.590; r = À0.597; r = À0.659, and r = À0.425) LIs, but no correlation with P53 LI (r = 0.364, P = 0.104; r = 0.350, P = 0.120; r = 0.379, P = 0.091; r = 0.390, P = 0.080). Data Conclusion:The ADC value can be used to evaluate cell proliferation and apoptosis indexes in a murine model of fibrosarcoma, employing the small-sample ROI as a reliable method.

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Photothermal Therapy as Adjuvant to Surgery in an Orthotopic Mouse Model of Human Fibrosarcoma

Cited by 11 publications

References 34 publications

Combination of [177Lu]Lu-DOTA-TATE Targeted Radionuclide Therapy and Photothermal Therapy as a Promising Approach for Cancer Treatment: In Vivo Studies in a Human Xenograft Mouse Model

Combination of [177Lu]Lu-DOTA-TATE Targeted Radionuclide Therapy and Photothermal Therapy as a Promising Approach for Cancer Treatment: In Vivo Studies in a Human Xenograft Mouse Model

Neoadjuvant Gold Nanoshell-Based Photothermal Therapy Combined with Liposomal Doxorubicin in a Mouse Model of Colorectal Cancer

Correlation of Apparent Diffusion Coefficient With Proliferation and Apoptotic Indexes in a Murine Model of Fibrosarcoma: Comparison of Four Methods for MRI Region of Interest Positioning

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