Photothermal treatment of glioblastoma cells based on plasmonic nanoparticles

Jalali, Bahareh Khaksar; Shik, Somayeh Salmani; Karimzadeh–Bardeei, Latifeh; Heydari, Esmaeil; Ara, Mohammad Hossein Majles

doi:10.1007/s10103-023-03783-5

Cited by 3 publications

(1 citation statement)

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“…The cell-mortality rates using GNRs similar to the SGNRs described here increased to 80% when using a lower laser power (1.5 W), but with a high concentration of GNRs present in the cell-culture medium during laser irradiation at 808 nm [22]. Additionally, PTT using a low-power laser was tested with a high concentration of nanoparticles, resulting in a cell-mortality rate of 60% after a PTT treatment [52]. Other strategies include the biofunctionalization of GNRs to target glioblastoma cells, which allowed the achievement of cell-mortality rates ranging from 20% to 60% after PTT [15,53].…”

Section: Discussionmentioning

confidence: 99%

Effectiveness of Gold Nanorods of Different Sizes in Photothermal Therapy to Eliminate Melanoma and Glioblastoma Cells

Domingo-Diez,

Souiade,

Manzaneda-González

et al. 2023

IJMS

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Gold nanorods are the most commonly used nanoparticles in photothermal therapy for cancer treatment due to their high efficiency in converting light into heat. This study aimed to investigate the efficacy of gold nanorods of different sizes (large and small) in eliminating two types of cancer cell: melanoma and glioblastoma cells. After establishing the optimal concentration of nanoparticles and determining the appropriate time and power of laser irradiation, photothermal therapy was applied to melanoma and glioblastoma cells, resulting in the highly efficient elimination of both cell types. The efficiency of the PTT was evaluated using several methods, including biochemical analysis, fluorescence microscopy, and flow cytometry. The dehydrogenase activity, as well as calcein-propidium iodide and Annexin V staining, were employed to determine the cell viability and the type of cell death triggered by the PTT. The melanoma cells exhibited greater resistance to photothermal therapy, but this resistance was overcome by irradiating cells at physiological temperatures. Our findings revealed that the predominant cell-death pathway activated by the photothermal therapy mediated by gold nanorods was apoptosis. This is advantageous as the presence of apoptotic cells can stimulate antitumoral immunity in vivo. Considering the high efficacy of these gold nanorods in photothermal therapy, large nanoparticles could be useful for biofunctionalization purposes. Large nanorods offer a greater surface area for attaching biomolecules, thereby promoting high sensitivity and specificity in recognizing target cancer cells. Additionally, large nanoparticles could also be beneficial for theranostic applications, involving both therapy and diagnosis, due to their superior detection sensitivity.

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Section: Discussionmentioning

confidence: 99%