Phototransformation of cyanine dye with two chromophores. Effects of oxygen and dye concentration

Silva, Érika R.; Pavanelli, André L.S.; Mostaço, Leila B.; Schaberle, Fábio A.; Galembeck, Sérgio E.; Gonçalves, Pablo José; Silva, Rodrigo Costa e; Ferreira, Lúcia Pires; Nekipelova, T. D.; Kostyukov, Alexey A.; Radchenko, A. Sh.; Штиль, А. А.; Кузьмин, В. А.; Borissevitch, Iouri

doi:10.1016/j.jphotochem.2017.08.063

Cited by 18 publications

(6 citation statements)

References 29 publications

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“…Therefore, the relative singlet oxygen quantum yield of the prepared FSNPs was evaluated indirectly by using a commercially available 1 O 2 indicator 1,3-diphenylisobenzofuran (DPBF) as a chemical trap . Distinctly, FSNP-A–C had a higher relative singlet oxygen quantum yield (Table S3) compared to the corresponding FSNP-a–c and free dye a–c (Φ MB = 0.52 as a reference), , while ICG or IR-787 had a value of only 0.008 or 0.007 . According to comparative tests for FSNP-C (Figure S8), the absorbance of DPBF decreased remarkably in the presence of FSNP-C under irradiation, giving the highest relative singlet oxygen quantum yield of Φ Δ = 0.085.…”

Section: Results and Discussionmentioning

confidence: 99%

Constructing a Local Hydrophobic Cage in Dye-Doped Fluorescent Silica Nanoparticles to Enhance the Photophysical Properties

et al. 2020

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Aggregation-caused quenching (ACQ) and poor photostability in aqueous media are two common problems for organic fluorescence dyes which cause a dramatic loss of fluorescence imaging quality and photodynamic therapy (PDT) failure. Herein, a local hydrophobic cage is built up inside near-infrared (NIR) cyanine-anchored fluorescent silica nanoparticles (FSNPs) in which a hydrophobic silane coupling agent (n-octyltriethoxysilane, OTES) is doped into FSNPs for the first time to significantly inhibit the ACQ effect and inward diffusion of water molecules. Therefore, the obtained optimal FSNP-C with OTES-modification can provide hydrophobic repulsive forces to effectively inhibit the π–π stacking interaction of cyanine dyes and simultaneously reduce the formation of strong oxidizing species (•OH and H2O2) in reaction with H2O, resulting in the best photostability (fluorescent intensity remained at 90.1% of the initial value after 300 s of laser scanning) and a high PDT efficiency on two- and three-dimensional (spheroids) HeLa cell culture models. Moreover, through molecular engineering (including increasing covalent anchoring sites and steric hindrance groups of cyanine dyes), FSNP-C exhibits the highest fluorescent intensity both in water solution (12.3-fold improvement compared to free dye) and living cells due to the limitation of molecular motion. Thus, this study provides an effectively strategy by combining a local hydrophobic cage and molecular engineering for NIR FSNPs in long-term bright fluorescence imaging and a stable PDT process.

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Section: Results and Discussionmentioning

confidence: 99%

Constructing a Local Hydrophobic Cage in Dye-Doped Fluorescent Silica Nanoparticles to Enhance the Photophysical Properties

et al. 2020

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“…To determine its ability to generate 1 O 2 , the indirect method for determination of 1 O 2 quantum yield, employing uric acid (UA) as the 1 O 2 sensor is used, which has proven to be a reliable, accurate, and low-cost method. − ,, By this method, the solution containing the vesicle system and UA was irradiated at 450 nm, which is the maximum absorption wavelength of the system, and electronic absorption spectra were recorded in intervals from 0 to 1320 s (Figure A). As control measurements, spectra were also recorded in similar experiments containing the Phe–Phe structure (Figure B); C6 (Figure C); perillyl alcohol (Figure D); a combination of C6 and perillyl alcohol (Figure E); and a combination of C6 and Phe–Phe structure (Figure F).…”

Section: Resultsmentioning

confidence: 99%

“…In the pro-oxidant activity assessment, UA was used as the O 2 1 sensor. , UA was dissolved in 2 mL of 1:1 of ethanol and water mixture to result in an absorbance between 1.0 and 1.8. Phe–Phe vesicles were dispersed in 2 mL of UA solution of UA and in a quartz cuvette.…”

Section: Materials and Methodologymentioning

confidence: 99%

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Photophysical Properties of Fluorescent Self-Assembled Peptide Nanostructures for Singlet Oxygen Generation

2020

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In this work, a drug delivery system for perillyl alcohol based on the peptide self-assembly containing 3-(2benzothiazolyl)-7-(diethylamino)coumarin (C6) as a fluorescent additive is obtained, and its photophysical characteristics as well as its release dynamics were studied by steady-state and time-resolved fluorescence spectroscopy. Results proved the dynamics of drug release from the peptide nanostructures and showed that the system formed by the self-assembled peptide and C6, along with perillyl alcohol, presents unique photophysical properties that can be exploited to generate singlet oxygen ( 1 O 2 ) upon irradiation, which is not achieved by the sole components. Through epifluorescence microscopy combined with time-correlated single photon counting fluorescence spectroscopy, the release mechanism was proven to occur upon peptide structure interconversion, which is controlled by environmental changes.

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“…Uma forma de mimetizar a membrana celular é utilizar micelas de diversos tipos de surfactantes. Tal método vem sendo usado há bastante tempo com diversos resultados importantes no campo da interação entre FS e micelas com diferentes características [37][38][39][40].…”

Section: Introductionunclassified

Estudos da interação de pontos quânticos com fotossensibilizadores de interesse em fotoquimioterapia na presença de sistemas biomiméticos

Pavanelli¹

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parte das exigências para obtenção do título de Doutor em Ciências, obtido no Programa de Pós-Graduação em Física Aplicada à Biologia e Medicina. Ribeirão Preto -SP v AGRADECIMENTOS Ao prof. Iouri pela paciência e pelos ensinamentos ao longo de todos estes anos. À minha esposa Janaina que me proibiu de desistir e que, pacientemente, ajudou a reconstruir o que estava em ruínas. Às minhas avós Eunice e Júlia pelo amor e carinho. À todos os meus familiares. Ao meu irmão Paulo e aos meus amigos Ícaro e Guilherme pela amizade e por muitas vezes emprestarem o ouvido para me ouvir reclamar. Aos colegas Danilo, Leonardo e Isamara pelos chás, conversas, discussões e lanches no GF. Ao técnico Adriano pelas tantas vezes em que ajudou nos experimentos e pela prosa. Ao Dr. Leandro Máximo e ao Prof. Roberto Santana por terem gentilmente fornecido os Pontos Quânticos utilizados neste trabalho. Á prof. Galina pelas conversas e dicas. À todos os colegas, professores e técnicos do Departamento de Física pela amizade e acolhimento durante tanto tempo. Aos amigos do 6º DP de Campinas pelo apoio e incentivo. À CAPES pela bolsa de doutorado. Gostaria de agradecer imensamente a todos aqueles que de alguma forma contribuíram para este trabalho. Obrigado! "Os caminhos que conduzem o homem ao saber são tão maravilhosos quanto o próprio saber." -Johannes Kepler viii RESUMO PAVANELLI, A. L.S. Estudos da interação de Pontos Quânticos com Fotossensibilizadores de interesse em Fotoquimioterapia na presença de sistemas biomiméticos. 2022. 149 p. Tese

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Phototransformation of cyanine dye with two chromophores. Effects of oxygen and dye concentration

Cited by 18 publications

References 29 publications

Constructing a Local Hydrophobic Cage in Dye-Doped Fluorescent Silica Nanoparticles to Enhance the Photophysical Properties

Constructing a Local Hydrophobic Cage in Dye-Doped Fluorescent Silica Nanoparticles to Enhance the Photophysical Properties

Photophysical Properties of Fluorescent Self-Assembled Peptide Nanostructures for Singlet Oxygen Generation

Estudos da interação de pontos quânticos com fotossensibilizadores de interesse em fotoquimioterapia na presença de sistemas biomiméticos

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