Photovoltaic cells as a highly efficient system for biomedical and electrochemical surface-enhanced Raman spectroscopy analysis

Niciński, Krzysztof; Witkowska, Evelin; Korsak, Dorota; Noworyta, Krzysztof; Trzcińska-Danielewicz, Joanna; Girstun, Agnieszka; Kamińska, Agnieszka

doi:10.1039/c8ra08319c

Cited by 11 publications

(8 citation statements)

References 111 publications

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“…As was mentioned above, the PV arrays were subsequently covered with silver by PVD process to achieve the SERS activity. The SERS efficiency (sensitivity, selectivity, and reproducibility) was demonstrated for p -aminothiophenol ( p -ATP), which is commonly used a standard probe molecule and has been described in details in our previous article 38 . The morphology of these SERS-active substrates was visualized by the atomic force microscopy (AFM) and scanning electron microscopy (SEM) techniques and is presented in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The developed SERS-active substrate exhibits the high enhancement factor (EF), high stability and reproducibility of the recorded spectra 38 . For p -aminothiophenol the enhancement factor (EF) of the Raman signal on a Ag/PV surface was estimated as high as 10 6 .…”

Section: Resultsmentioning

confidence: 99%

“…The prepared SERS platform is ready for use. In order to get acquainted with their extensive characteristics, we recommend to get familiarized with our previous work 38 .

Figure 1Scheme of the preparation of SERS substrate, microfluidic system, analyte and measurement. Main steps involve cleaning ( a ), drying ( b ), sputtering of thin layer of silver ( c ), the process of preparing microfluidic system ( d ), analyte and buffer preparation followed by the separation of tumor cells ( e ) and finally the measurement takes place ( f ).

Figure 2( A ) Setup used for CTCs separation from whole blood, ( B ) top view of microfluidic device, ( C ) cross-sectional view of microchannel (dimensions in the figure are given in millimeters), and ( D ) SEM image of SERS-active platform incorporated into the detection area chambers (DA) of microfluidic device.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Detection of circulating tumor cells in blood by shell-isolated nanoparticle – enhanced Raman spectroscopy (SHINERS) in microfluidic device

Niciński

Krajczewski

Kudelski

et al. 2019

Sci Rep

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Isolation and detection of circulating tumor cells (CTCs) from human blood plays an important role in non- invasive screening of cancer evolution and in predictive therapeutic treatment. Here, we present the novel tool utilizing: (i) the microfluidic device with (ii) incorporated photovoltaic (PV) based SERS-active platform, and (iii) shell-isolated nanoparticles (SHINs) for simultaneous separation and label-free analysis of circulating tumour cells CTCs in the blood specimens with high specificity and sensitivity. The proposed microfluidic chip enables the efficient size – based inertial separation of circulating cancer cells from the whole blood samples. The SERS-active platform incorporated into the microfluidic device permits the label-free detection and identification of isolated cells through the insight into their molecular and biochemical structure. Additionally, the silver nanoparticles coated with an ultrathin shell of silica (Ag@SiO 2 ) was used to improve the detection accuracy and sensitivity of analysed tumor cells via taking advantages of shell-isolated nanoparticle-enhanced Raman spectroscopy (SHINERS). The empirical analysis of SHINERS spectra revealed that there are some differences among studied (HeLa), renal cell carcinoma (Caki-1), and blood cells. Unique SHINERS features and differences in bands intensities between healthy and cancer cells might be associated with the variations in the quantity and quality of molecules such as lipid, protein, and DNA or their structure during the metastasis cancer formation. To demonstrate the statistical efficiency of the developed method and improve the differentiation for circulating tumors cells detection the principal component analysis (PCA) has been performed for all SHINERS data. PCA method has been applied to recognize the most significant differences in SHINERS data among the three analyzed cells: Caki-1, HeLa, and blood cells. The proposed approach challenges the current multi-steps CTCs detection methods in the terms of simplicity, sensitivity, invasiveness, destructivity, time and cost of analysis, and also prevents the defragmentation/damage of tumor cells and thus leads to improving the accuracy of analysis. The results of this research work show the potential of developed SERS based tool for the separation of tumor cells from whole blood samples in a simple and minimally invasive manner, their detection and molecular characterization using one single technology.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

“…The prepared SERS platform is ready for use. In order to get acquainted with their extensive characteristics, we recommend to get familiarized with our previous work 38 .

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Detection of circulating tumor cells in blood by shell-isolated nanoparticle – enhanced Raman spectroscopy (SHINERS) in microfluidic device

Niciński

Krajczewski

Kudelski

et al. 2019

Sci Rep

Self Cite

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“…In order to enhance the Raman signal of bacterial cells we used polycrystalline silicon covered with silicon nitride and Ag nanostructures. This previously developed SERS substrate [43] is based on PV panels obtained from Bruk-Bet Solar Tarnów, Poland as a post-production residue.…”

Section: Sers Substratesmentioning

confidence: 99%

“…In this paper, we show a new approach applying silver/photovoltaic-based (Ag/PV) SERS substrates [43] in the detection of foodborne bacteria, namely thermotolerant Campylobacter spp., in different poultry samples. The topic of Campylobacter spp.…”

mentioning

confidence: 99%

Nanoplasmonic sensor for foodborne pathogens detection. Towards development of ISO‐SERS methodology for taxonomic affiliation of Campylobacter spp.

Witkowska

Niciński

Korsak

et al. 2020

Journal of Biophotonics

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According to EU summary report on zoonoses, zoonotic agents and food-borne outbreaks in 2017, Campylobacter was the most commonly reported gastrointestinal bacterial pathogen in humans in the EU. Unfortunately, the standard methods for the detection of thermotolerant Campylobacter spp. in foods are time-consuming.Additionally, the qualified staff is obligatory.For this reason, new methods of pathogens detection are needed. The present work demonstrates that surface-enhanced Raman scattering (SERS) is a reliable and fast method for detection of Campylobacter spp. in food samples. The proposed method combines the SERS measurements performed on an Ag/Si substrate with two initial steps of the ISO standard procedure. Finally, the principal component analysis (PCA) allows for statistical classification of the studied bacteria. By applying the proposed ISO-SERS-PCA method in the case of Campylobacter bacteria the total detection time may be reduced from 7 to 8 days required by ISO method to 3 to 4 days in the case of SERS-based approach. K E Y W O R D SCampylobacter spp., foodborne bacteria, ISO, PCA, SERS, surface-enhanced Raman spectroscopy

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Association between grade brain tumors and the interleukin‐10 receptor subunit alpha based on surface‐enhanced Raman spectroscopy and multivariate analysis

Kowalska

Czaplicka

Nowicka

et al. 2021

J Raman Spectroscopy

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We present herein a label-free method based on surface-enhanced Raman spectroscopy (SERS) that was performed using a silicon-based SERS platform. The differentiation between primary and secondary brain tumors SERS data was completed partial least squares (PLS) method with a very high 85% of accuracy (in only first and second factors), whereas calculated using principal component analysis (PCA) method gives 74% (in two consecutive components). Additionally, due to the fact that the interleukin-10 (IL-10) cytokine receptor may act in cancer as both an immunosuppressive and an immunostimulant factor, the correlation between the tumor grading and the cytokine receptor are presented. Obtained data indicate that the function of IL-10 cytokine receptor subunit alpha most probably depends or is closely related to the tumor stage.

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Photovoltaic cells as a highly efficient system for biomedical and electrochemical surface-enhanced Raman spectroscopy analysis

Abstract: We have developed a conductive SERS platform based on photovoltaic materials (PV) covered with a thin layer of silver, especially useful in electrochemical SERS analysis.

Cited by 11 publications

References 111 publications

Detection of circulating tumor cells in blood by shell-isolated nanoparticle – enhanced Raman spectroscopy (SHINERS) in microfluidic device

Detection of circulating tumor cells in blood by shell-isolated nanoparticle – enhanced Raman spectroscopy (SHINERS) in microfluidic device

Nanoplasmonic sensor for foodborne pathogens detection. Towards development of ISO‐SERS methodology for taxonomic affiliation of Campylobacter spp.

Association between grade brain tumors and the interleukin‐10 receptor subunit alpha based on surface‐enhanced Raman spectroscopy and multivariate analysis

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