Phthalate induced oxidative stress and DNA damage in earthworms (Eisenia fetida)

Song, Peipei; Gao, Jintao; Li, Xianxu; Zhang, Cui; Zhu, Lusheng; Wang, Jinhua; Wang, Jun

doi:10.1016/j.envint.2019.04.074

Cited by 150 publications

(34 citation statements)

References 42 publications

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“…The obtained results are in line with previous studies in other organisms, which have confirmed that BBP can induce different types of damage such as apoptosis 48 , genotoxicity 49 , oxidative stress 50 , stress response activation 45 , or endocrine disruption 14 . Although there are studies in invertebrates showing the impact on development and other physiological processes 39 , 51 , most of them did not focus on the putative mode of action, with only a few of them trying to delve into the response mechanisms.…”

Section: Discussionsupporting

confidence: 91%

Analysis of the impact of three phthalates on the freshwater gastropod Physella acuta at the transcriptional level

Prieto-Amador

Caballero

Martínez-Guitarte

2021

Sci Rep

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Plastic pollution is one of the leading environmental problems. Phthalates are widely used plastic additives released into the environment. Although the effects of phthalates on vertebrates have been extensively studied, there is a knowledge gap regarding their effects on invertebrates. This work analyzes the impact of three phthalates, diethyl phthalate (DEP), benzyl butyl phthalate (BBP), and bis-(2-ethylhexyl) phthalate (DEHP), on the gastropod Physella acuta at the molecular level to establish the putative pathways involved in its response to them. By real-time PCR, we obtained the expression profile of 30 genes in animals exposed for 1 week to 0.1, 10, and 1000 μg/L of each phthalate. The genes cover DNA repair, detoxification, apoptosis, oxidative and stress responses, immunity, energy reserves, and lipid transport. The results show that while DEP and DEHP did not alter the mRNA levels, BBP modulated almost all the analyzed genes. It can be concluded that the impact of BBP is extensive at the molecular level. However, it cannot be dismissed that the increase in transcriptional activity is a general response due to this compound’s well-known role as an endocrine disruptor. Additional research is needed to elucidate the differences observed in the impact of these compounds on the gastropod P. acuta.

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Section: Discussionsupporting

confidence: 91%

Analysis of the impact of three phthalates on the freshwater gastropod Physella acuta at the transcriptional level

Prieto-Amador

Caballero

Martínez-Guitarte

2021

Sci Rep

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“…Moreover, inappropriate production of other oxidative stress markers, such as malondialdehyde (MDA) and superoxide dismutase (SOD), can occur under exposure to DEHP concentration [ 33 ]. Previous studies showed a positive correlation between phthalates exposure and MDA concentration [ 34 – 36 ]. Both DEHP and monobutyl phthalate (MnBP) exposures were involved in increase of oxidative stress markers and fatty acid oxidation [ 37 ].…”

Section: Introductionmentioning

confidence: 99%

Relationship of Urinary Phthalate Metabolites with Cardiometabolic Risk Factors and Oxidative Stress Markers in Children and Adolescents

Hashemi

Amin

Chavoshani

et al. 2021

Journal of Environmental and Public Health

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Introduction. Studies have proved that exposure of adults to phthalates might be related to cardiometabolic risk factors and changes in markers of oxidative stress. Such studies conducted on school-age children and adolescents are limited and fail to assess the simultaneous effect of phthalates on these risk factors and oxidative stress markers. Therefore, it was attempted to identify the relationship of urinary phthalate metabolites with cardiometabolic risk factors and oxidative stress markers in children and adolescents. Methods. In this cross-sectional study, 108 children and adolescents, living in Isfahan industrial city of Iran, were examined. Urine samples taken from the participants were analyzed for mono-butyl phthalate (MBP), mono-benzyl phthalate (MBzP), mono-(2-ethylhexyl) phthalate (MEHP), mono-(2-ethyl-5-hydroxyhexyl) phthalate (MEHHP), mono-(2-ethyl-5-exohexyl) phthalate (MEOHP), and mono-methyl phthalate (MMP). Results. Results showed that, among phthalate metabolites, MBP had the highest concentration, followed by MBzP, MEOHP, MEHHP, MEHP, and MMP. Concentrations of these metabolites had a significant relationship with some of the cardiometabolic risk factors including systolic blood pressure (SBP), fasting blood sugar (FBS), and triglycerides (TG) ( p < 0.05). Furthermore, the crude and adjusted linear regression models indicated the significant association of phthalate metabolites with superoxide dismutase (SOD), malondialdehyde (MDA), and homeostasis model assessment of insulin resistance (HOMA-IR) ( p < 0.05). Conclusion. Although urinary phthalate concentrations could not exactly reflect the long-term exposure level in the studied age groups, the consumption of phthalate-free products during childhood and adolescent development shall be assumed helpful in maintaining a healthy lifestyle. To confirm these findings and develop effective intervention strategies, it would be necessary to perform longitudinal studies on diverse population.

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“…Previous studies in other organisms have con rmed that BBP can induce different types of damage such as apoptosis 41 , genotoxicity 42 , oxidative stress 43 , stress response activation 44 , or endocrine disruption 13 . Although there are studies in invertebrates showing the impact on development and other physiological processes 38,45 , most of them did not focus on the putative mode of action, with only a few of them trying to delve into the mechanisms of response.…”

Section: Discussionmentioning

confidence: 99%

Analysis of the Impact of Three Phthalates on the Freshwater Gastropod Physella Acuta at the Transcriptional Level

Prieto-Amador

Caballero

Martínez-Guitarte

2021

Preprint

View full text Add to dashboard Cite

Plastic pollution is one of the leading environmental problems. Phthalates are widely used plastic additives released into the environment. Although the phthalates' effects have been extensively studied on vertebrates, there is a gap in knowledge on their effects on invertebrates. This work analyzes the impact of three phthalates, diethyl phthalate (DEP), benzyl butyl phthalate (BBP), and bis-(2-ethylhexyl) phthalate (DEHP), on the gastropod Physella acuta at the molecular level to establish the putative pathways involved in its response to them. By real-time PCR, we obtained the expression profile of thirty genes in one-week exposed animals at 0.1, 10, and 1000 µg/L. The genes cover the DNA repairing mechanism, detoxification mechanisms, apoptosis, oxidative and stress responses, immunity, energy reserves, and lipid transport. The results show that while DEP and DEHP did not cause alteration of the mRNA levels, BBP modulates almost all the genes tested. It can be concluded that the impact of BBP is extensive at the molecular level. However, it cannot be dismissed that the increase in transcriptional activity is a general response due to this compound’s well-known role as an endocrine disruptor. Additional research is needed to elucidate the differences observed in the impact of these compounds on this gastropod.

show abstract

Phthalate induced oxidative stress and DNA damage in earthworms (Eisenia fetida)

Cited by 150 publications

References 42 publications

Analysis of the impact of three phthalates on the freshwater gastropod Physella acuta at the transcriptional level

Analysis of the impact of three phthalates on the freshwater gastropod Physella acuta at the transcriptional level

Relationship of Urinary Phthalate Metabolites with Cardiometabolic Risk Factors and Oxidative Stress Markers in Children and Adolescents

Analysis of the Impact of Three Phthalates on the Freshwater Gastropod Physella Acuta at the Transcriptional Level

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