Phycobiliproteins from Arthrospira Platensis (Spirulina): A New Source of Peptides with Dipeptidyl Peptidase-IV Inhibitory Activity

Li, Yuchen; Aiello, Gilda; Bollati, Carlotta; Bartolomei, Martina; Arnoldi, Anna; Lammi, Carmen

doi:10.3390/nu12030794

Cited by 53 publications

(29 citation statements)

References 31 publications

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“…In addition, SP reduces the DPP-IV activity by 64.6% in vitro and 31.7% on Caco-2 cells, while ST reduces it by 74.2% in vitro and 39.8% on Caco-2 cells (at the dose of 5 mg/mL) [ 14 ]. In addition, the tryptic digest of phycobiliprotein purified from spirulina inhibits the DPP-IV activity by 95.8% in vitro, whereas it inhibits it by 44% in situ at 5 mg/mL [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

Investigation of Chlorella pyrenoidosa Protein as a Source of Novel Angiotensin I-Converting Enzyme (ACE) and Dipeptidyl Peptidase-IV (DPP-IV) Inhibitory Peptides

Aiello

Fassi

et al. 2021

Nutrients

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Chlorella pyrenoidosa (C. pyrenoidosa) is a microalgae species with a remarkably high protein content that may potentially become a source of hypotensive and hypoglycemic peptides. In this study, C. pyrenoidosa proteins were extracted and hydrolyzed overnight with pepsin and trypsin with final degrees of hydrolysis of 18.7% and 35.5%, respectively. By LC-MS/MS, 47 valid peptides were identified in the peptic hydrolysate (CP) and 66 in the tryptic one (CT). At the concentration of 1.0 mg/mL, CP and CT hydrolysates inhibit in vitro the angiotensin-converting enzyme (ACE) activity by 84.2 ± 0.37% and 78.6 ± 1.7%, respectively, whereas, tested at cellular level at the concentration of 5.0 mg/mL, they reduce the ACE activity by 61.5 ± 7.7% and 69.9 ± 0.8%, respectively. At the concentration of 5.0 mg/mL, they decrease in vitro the DPP-IV activity by 63.7% and 69.6% and in Caco-2 cells by 38.4% and 42.5%, respectively. Short peptides (≤10 amino acids) were selected for investigating the potential interaction with ACE and DPP-IV by using molecular modeling approaches and four peptides were predicted to block both enzymes. Finally, the stability of these peptides was investigated against gastrointestinal digestion.

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Section: Discussionmentioning

confidence: 99%

Investigation of Chlorella pyrenoidosa Protein as a Source of Novel Angiotensin I-Converting Enzyme (ACE) and Dipeptidyl Peptidase-IV (DPP-IV) Inhibitory Peptides

Aiello

Fassi

et al. 2021

Nutrients

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“…[4] Due to an increasing concern in animal welfare and the rising environmental awareness, [5] the consumption of vegetable products is becoming a common trend. In this way, a growing body of evidence has underlined several biological actions such as immune, [6,7] antioxidant [6,8] and metabolic [9][10][11] effects of different vegetable protein hydrolysates by in vitro or pre-clinical approaches. We have previously shown that Lupinus angustifolius protein hydrolysates (LPHs), generated by hydrolyzation with the commonly used food-grade endopeptidase Alcalase 2.4 L, inhibit the enzymatic activity inflammatory enzymes phospholipase A2, cyclooxygenase 2, thrombin, and transglutaminase in a cell-free system.…”

Section: Introductionmentioning

confidence: 99%

Safety and Efficacy of a Beverage Containing Lupine Protein Hydrolysates on the Immune, Oxidative and Lipid Status in Healthy Subjects: An Intervention Study (the Lupine‐1 Trial)

Cruz‐Chamorro

Álvarez-Sánchez

Álvarez-Ríos

et al. 2021

Molecular Nutrition Food Res

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Scope: We have previously demonstrated the anti-inflammatory and antioxidant properties of in vitro administered Lupinus angustifolius protein hydrolysates (LPHs) on human peripheral blood mononuclear cells (PBMCs). This study aims to evaluate the safety and efficacy of a beverage containing LPHs (LPHb) on the immune, oxidative and metabolic status of healthy subjects. Methods and Results: In this open-label intervention, 33 participants daily ingest a LPHb containing 1 g LPHs for 28 days. Biochemical parameters are assayed in fasting peripheral blood and urine samples before, during (14 days) and after LPHb ingestion. Participants' health status and the immune and antioxidant responses of PBMCs are also evaluated throughout the trial. The LPHb ingestion is safe and effective in both increasing the anti-/proinflammatory response of PBMCs and improving the cellular anti-oxidant capacity. LPHb also reduces the low-density lipoprotein-cholesterol (LDL-C)/high-density lipoprotein-cholesterol (HDL-C) atherogenic index. LPHb effect is particularly beneficial on decreasing not only the LDL-C/HDL-C index but also serum total cholesterol levels in the male cohort that shows the highest baseline levels of well-known cardiovascular risk factors. Conclusion: This is the first study to show the pleiotropic actions of a lupine bioactive peptides-based functional food on key steps of atherosclerosis including inflammation, oxidative stress, and cholesterol metabolism.

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“…Glucagon-like peptide-1 (GLP-1) is synthesized in the L-cells of the small intestine as a glucagon-containing precursor peptide, and is involved in the inhibition of glucagon secretion and the promotion of insulin secretion in the pancreas [ 28 ]. The N-terminal dipeptides of the GLP-1 are cleaved by DPP-IV (EC 3.4.14.5), and the cleaved GLP-1 acts as an antagonist to the above physiological effects [ 29 ]. Therefore, the inhibition of DPP-IV is an effective way to prevent and treat diabetes.…”

Section: Resultsmentioning

confidence: 99%

Study on the Mechanism of the Blood-Glucose-Lowering Effect of Collagen Peptides from Sturgeon By-Products

et al. 2021

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In a previous study, we found that the collagen peptides prepared from the by-products of Bester sturgeon had an inhibitory effect on elevated blood glucose levels in a glucose tolerance test with ICR mice. In the present study, we examine the mechanism of the effect of sturgeon collagen peptides (SCPs) in detail. When glucose was orally administered to mice along with the SCPs, it was found that the glucose remained in the stomach for a longer time. In the above tests, the amount of glucose excreted in the feces of mice also increased. On the contrary, it was revealed that the SCPs have a dipeptidyl-peptidase-IV (DPP-IV) inhibitory ability in an in vitro test. In subsequent oral and intravenous glucose administration tests, glucagon-like peptide-1 (GLP-1) and insulin levels in the blood of mice were maintained at high levels. These results suggested the following three mechanisms: SCPs slow the rate of transportation of glucose from the stomach into the small intestine, resulting in delayed glucose absorption; SCPs suppress the absorption of glucose in the small intestine and excrete it from the body; SCPs inhibit DPP-IV in the blood and maintain a high GLP-1 level in blood, which in turn stimulates insulin secretion.

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Phycobiliproteins from Arthrospira Platensis (Spirulina): A New Source of Peptides with Dipeptidyl Peptidase-IV Inhibitory Activity

Cited by 53 publications

References 31 publications

Investigation of Chlorella pyrenoidosa Protein as a Source of Novel Angiotensin I-Converting Enzyme (ACE) and Dipeptidyl Peptidase-IV (DPP-IV) Inhibitory Peptides

Investigation of Chlorella pyrenoidosa Protein as a Source of Novel Angiotensin I-Converting Enzyme (ACE) and Dipeptidyl Peptidase-IV (DPP-IV) Inhibitory Peptides

Safety and Efficacy of a Beverage Containing Lupine Protein Hydrolysates on the Immune, Oxidative and Lipid Status in Healthy Subjects: An Intervention Study (the Lupine‐1 Trial)

Study on the Mechanism of the Blood-Glucose-Lowering Effect of Collagen Peptides from Sturgeon By-Products

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