Phycobilisomes from Blue-Green and Red Algae

Gantt, Elisabeth; Lipschultz, Claudia A.; Grabowski, Joseph; Zimmerman, Burke K.

doi:10.1104/pp.63.4.615

Cited by 193 publications

(64 citation statements)

References 9 publications

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“…I lb). The latter difference spectrum was similar to the fluorescence emission spectrum of phycobilisomes extracted from A. nidulans (5). The two emission bands might have been emitted from two kinds of allophycocyanin (6).…”

Section: Effects Of Chilling Treatmentsupporting

confidence: 49%

Chilling Susceptibility of the Blue-green Alga Anacystis nidulans

Ono¹,

Murata²

1981

Plant Physiol.

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Effects of chilling treatment on the photosynthetic activities and the Ught-absorption and fluorescence spectra were investigated in intact cells of the blue-green alga Anacystis nidulans that were grown at different temperatures. When the algal cells grown at 38 C were treated at 0 C for 10 minutes, the photosynthesis and the Hill reaction with 1,4-benzoquinone were significantly inactivated and the light-absorption spectrum of carotenoids was modified. These parameters showed very similar temperature dependencies in the chilling susceptibility and the temperature regions critical for the susceptibility depended on the growth temperature. The midpoint values for the critical temperature regions were 4, 6, and 12 C in cells grown at 28, 33, and 38 C, respectively. It is proposed that a common mechanism would underlie the chiUling susceptibility of the photosynthesis, the Hill reaction, and the carotenoid absorption spectrum. The decoupling of excitation transfer from allophycocyanin to chlorophyll a at the chilling temperatures occurred very slowly and is attributed to a somewhat different mechanism of the chilling susceptibilty. Some higher plants are markedly susceptible to chilling temperatures (12). These plants are called "chilling-sensitive plants" and this phenomenon is named "chilling injury." It has been proposed, but not yet fully proved, that chilling injury is the result of the phase change of lipids in the cellular membranes at low temperatures (12,19). Some bactena also die when they are exposed to temperatures near 0 C (14, 18). This phenomenon is termed "cold shock." It is assumed that cold shock is induced by a temperature-dependent phase change of the membrane lipids (7, 18). It seems plausible that similar mechanisms underlie the chilling injury of higher plants and the cold shock of bacteria.Blue-green algae are prokaryotic organisms but are specified by their autotrophy on the plant-type oxygenic photosynthesis (24). They seem to possess an evolutional position between bacteria and plants. Therefore, the chilling susceptibility of the blue-green algae can be a link which connects chilling injury and cold shock.A number of studies have been done to reveal that a blue-green alga, Anacystis nidulans, is susceptible to chilling (1,3,4,8,20 Kratz and Myers (9) bubbled with air enriched by 1% CO. Continuous illumination light with an intensity of 6,000 lux was provided from incandescent lamps. The cells were kept growing at constant temperatures for more than 1 week before use. Growth temperatures were 28.0, 33.0, 35.5, 38.0, and 42.0 C. Accuracy of the growth temperature was within 0.1 C. Cultures at the late-logarithmic phase were used for the experiments.The algal cells were harvested by centrifugation at 5,000g for 5 min at the growth temperature. They were suspended in the fresh culture medium, the temperature of which had been adjusted to the growth condition. The cell density was in a range of 5 to 10 ytg Chl/ml. For chilling treatments of the cells at various temperatures for va...

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Section: Effects Of Chilling Treatmentsupporting

confidence: 49%

Chilling Susceptibility of the Blue-green Alga Anacystis nidulans

Ono¹,

Murata²

1981

Plant Physiol.

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“…This was determined by their recovery from the l-2 M sucrose interface of the same type of gradient as used for isolating phycobilisomes [12]. Under these sedimentation conditions thylakoid fragments with attached phycobilisomes sedimented higher on the gradient than the PS II-phycobilisomes.…”

Section: Resultsmentioning

confidence: 99%

“…The reduction of the chl content in the PS , phycobilisomes ( ---) isolated wi h Triton X-100 [12], and thylakoid (---) in SPC medium at room temperature normalized at 550 nm. ( t ) Difference spectrum showing chlorophyll content n PS II-phycobilisome particles.…”

Section: Resultsmentioning

confidence: 99%

Isolation of oxygen‐evolving phycobilisome—photosystem II particles from Porphyridium cruentum

Clément‐Métral

Gantt

1983

FEBS Letters

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Photosystem II phycobilisome particles were isolated from the red alga Porphyridium cruentum in a sucrose-phosphate-citrate-magnesium medium. These particles showed high rates of oxygen evolution in the water-to-silicomolybdate reaction (2300 /tmol 02. mg chl-' . h-l). Photosystem I, according to fluorescence emission spectra (-196'(Z), was greatly reduced or absent. The results demonstrate a close physical relationship of photosystem II with the phycobilisomes which are the major light harvesting antennae in this organism.

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“…Under higher ionic conditions however, the initial uncoupling can occur between PC and APC. These dissociation patterns are thought to be species specific (Gantt et al 1979, Grossman et al 1993. Phycoerythrins are the most abundant PBP in many red algae and they display rapid binding and dissociation abilities (Algarra et al 1990, Glazer 1994, Talarico 1996.…”

Section: Algal Materials and Culture Conditionsmentioning

confidence: 99%

Phycobilisome composition in Chondrus crispus (Gigartinales, Rhodophyta) from a wild type strain and its vegetatively derived green mutant

Cornish¹,

Garbary²

2013

ALGAE

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Intact phycobilisomes from a wild-type red Chondrus crispus and its vegetatively derived green mutant were isolated by centrifugation through a discontinuous sucrose density gradient. Pigment composition was subsequently characterized by spectrophotometry. Vegetative thalli of the two strains grown together for six months in the laboratory resulted in different pigment profiles. Two pigmented phycobilisome bands appeared in the sucrose gradient of the wild-type alga, a purple coloured one, and a pink one, whereas only a single blue band appeared in the gradient of the green mutant. Spectrophotometric and fluorescence analyses identified the phycobiliprotein composition of the purple band as the typical phycoerythrin-phycocyanin-allophycocyanin complement in the wild-type, but there was no detectable phycoerythrin present in the blue band of the green mutant. Sodium dodecyl sulphate, preparative polyacrylamide gel electrophoresis analysis confirmed the presence of allophycocyanin subunits in all extracts, but firm evidence of an R-phycoerythrin linker polypeptide in the blue band was missing. These results highlight the ability of C. crispus to adapt to a phycoerythrin deficiency by adjusting light harvesting pigment ratios.

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Phycobilisomes from Blue-Green and Red Algae

Cited by 193 publications

References 9 publications

Chilling Susceptibility of the Blue-green Alga Anacystis nidulans

Chilling Susceptibility of the Blue-green Alga Anacystis nidulans

Isolation of oxygen‐evolving phycobilisome—photosystem II particles from Porphyridium cruentum

Phycobilisome composition in Chondrus crispus (Gigartinales, Rhodophyta) from a wild type strain and its vegetatively derived green mutant

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