Phyllosphere bacterial communities of trichome-bearing and trichomeless Arabidopsis thaliana leaves

Reisberg, Eva E.; Hildebrandt, U.; Riederer, Markus; Hentschel, Ute

doi:10.1007/s10482-011-9669-8

Cited by 47 publications

(34 citation statements)

References 40 publications

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“…The total wax load and composition per surface area described in this study for this mutant closely matched published values for wild-type leaves (Lü et al, 2009;Bourdenx et al, 2011) and supports previous findings where gl1 was shown to have relatively small differences in wax composition per fresh weight as compared with the wild type (Xia et al, 2010). Moreover, the leaf wax composition in this study parallels that found by Reisberg et al (2012), assuming they grouped straight and branched alcohols together.…”

Section: Discussionsupporting

confidence: 90%

Composition and Physiological Function of the Wax Layers Coating Arabidopsis Leaves: β-Amyrin Negatively Affects the Intracuticular Water Barrier

2012

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Plants prevent dehydration by coating their aerial, primary organs with waxes. Wax compositions frequently differ between species, organs, and developmental stages, probably to balance limiting nonstomatal water loss with various other ecophysiological roles of surface waxes. To establish structure-function relationships, we quantified the composition and transpiration barrier properties of the gl1 mutant leaf waxes of Arabidopsis (Arabidopsis thaliana) to the necessary spatial resolution. The waxes coating the upper and lower leaf surfaces had distinct compositions. Moreover, within the adaxial wax, the epicuticular layer contained more wax and a higher relative quantity of alkanes, whereas the intracuticular wax had a higher percentage of alcohols. The wax formed a barrier against nonstomatal water loss, where the outer layer contributed twice as much resistance as the inner layer. Based on this detailed description of Arabidopsis leaf waxes, structure-function relationships can now be established by manipulating one cuticle component and assessing the effect on cuticle functions. Next, we ectopically expressed the triterpenoid synthase gene AtLUP4 (for lupeol synthase4 or b-amyrin synthase) to compare water loss with and without added cuticular triterpenoids in Arabidopsis leaf waxes. b-Amyrin accumulated solely in the intracuticular wax, constituting up to 4% of this wax layer, without other concomitant changes of wax composition. This triterpenoid accumulation caused a significant reduction in the water barrier effectiveness of the intracuticular wax.

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Section: Discussionsupporting

confidence: 90%

Composition and Physiological Function of the Wax Layers Coating Arabidopsis Leaves: β-Amyrin Negatively Affects the Intracuticular Water Barrier

2012

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“…However, consistent among all plants, regardless of growth conditions, was the predominance of the Proteobacteria and Firmicutes phyla. These phyla comprise the majority of bacteria in the phyllosphere of other agricultural and native plant species (8, 11, 13, 14, 30). This result is notable, given the likelihood that the plants in the agricultural field were likely exposed to very different bacterial species than those in the environmental chamber.…”

Section: Discussionmentioning

confidence: 99%

Phyllosphere Microbiota Composition and Microbial Community Transplantation on Lettuce Plants Grown Indoors

Williams

Marco

2014

mBio

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The aerial surfaces of plants, or phyllosphere, are microbial habitats important to plant and human health. In order to accurately investigate microbial interactions in the phyllosphere under laboratory conditions, the composition of the phyllosphere microbiota should be representative of the diversity of microorganisms residing on plants in nature. We found that Romaine lettuce grown in the laboratory contained 10- to 100-fold lower numbers of bacteria than age-matched, field-grown lettuce. The bacterial diversity on laboratory-grown plants was also significantly lower and contained relatively higher proportions of Betaproteobacteria as opposed to the Gammaproteobacteria-enriched communities on field lettuce. Incubation of field-grown Romaine lettuce plants in environmental growth chambers for 2 weeks resulted in bacterial cell densities and taxa similar to those on plants in the field but with less diverse bacterial populations overall. In comparison, the inoculation of laboratory-grown Romaine lettuce plants with either freshly collected or cryopreserved microorganisms recovered from field lettuce resulted in the development of a field-like microbiota on the lettuce within 2 days of application. The survival of an inoculated strain of Escherichia coli O157:H7 was unchanged by microbial community transfer; however, the inoculation of E. coli O157:H7 onto those plants resulted in significant shifts in the abundance of certain taxa. This finding was strictly dependent on the presence of a field-associated as opposed to a laboratory-associated microbiota on the plants. Phyllosphere microbiota transplantation in the laboratory will be useful for elucidating microbial interactions on plants that are important to agriculture and microbial food safety.

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“…Accordingly, the primary function of cuticles is to limit nonstomatal water loss and, thus, to protect plants against drought stress (Burghardt and Riederer, 2006). However, plant cuticles also play roles in minimizing the adhesion of dust, pollen, and spores (Barthlott and Neinhuis, 1997), protecting tissues from UV radiation (Krauss et al, 1997;Solovchenko and Merzlyak, 2003), mediating biotic interactions with microbes (Carver and Gurr, 2006;Leveau, 2006;Hansjakob et al, 2010Hansjakob et al, , 2011Reisberg et al, 2012) as well as insects (Eigenbrode and Espelie, 1995;Müller and Riederer, 2005), and preventing deleterious fusions between different plant organs (Tanaka and Machida, 2013).…”

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confidence: 99%

Localization of the Transpiration Barrier in the Epi- and Intracuticular Waxes of Eight Plant Species: Water Transport Resistances Are Associated with Fatty Acyl Rather Than Alicyclic Components

Jetter

Riederer²

2015

Plant Physiol.

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170

163

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Plant cuticular waxes play a crucial role in limiting nonstomatal water loss. The goal of this study was to localize the transpiration barrier within the layered structure of cuticles of eight selected plant species and to put its physiological function into context with the chemical composition of the intracuticular and epicuticular wax layers. Four plant species (Tetrastigma voinierianum, Oreopanax guatemalensis, Monstera deliciosa, and Schefflera elegantissima) contained only very-long-chain fatty acid (VLCFA) derivatives such as alcohols, alkyl esters, aldehydes, and alkanes in their waxes. Even though the epicuticular and intracuticular waxes of these species had very similar compositions, only the intracuticular wax was important for the transpiration barrier. In contrast, four other species (Citrus aurantium, Euonymus japonica, Clusia flava, and Garcinia spicata) had waxes containing VLCFA derivatives, together with high percentages of alicyclic compounds (triterpenoids, steroids, or tocopherols) largely restricted to the intracuticular wax layer. In these species, both the epicuticular and intracuticular waxes contributed equally to the cuticular transpiration barrier. We conclude that the cuticular transpiration barrier is primarily formed by the intracuticular wax but that the epicuticular wax layer may also contribute to it, depending on species-specific cuticle composition. The barrier is associated mainly with VLCFA derivatives and less (if at all) with alicyclic wax constituents. The sealing properties of the epicuticular and intracuticular layers were not correlated with other characteristics, such as the absolute wax amounts and thicknesses of these layers.

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Phyllosphere bacterial communities of trichome-bearing and trichomeless Arabidopsis thaliana leaves

Cited by 47 publications

References 40 publications

Composition and Physiological Function of the Wax Layers Coating Arabidopsis Leaves: β-Amyrin Negatively Affects the Intracuticular Water Barrier

Composition and Physiological Function of the Wax Layers Coating Arabidopsis Leaves: β-Amyrin Negatively Affects the Intracuticular Water Barrier

Phyllosphere Microbiota Composition and Microbial Community Transplantation on Lettuce Plants Grown Indoors

Localization of the Transpiration Barrier in the Epi- and Intracuticular Waxes of Eight Plant Species: Water Transport Resistances Are Associated with Fatty Acyl Rather Than Alicyclic Components

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