Phyllospheric Microbial Composition and Diversity of the Tobacco Leaves Infected by Didymella segeticola

Huang, Yü; Wang, Han-Cheng; Cai, Lun; Li, Wenhong; Pan, Daiwei; Xiang, Ligang; Xian-kun, SU; Li, Zhong; Adil, Muhammad Faheem; Shamsi, Imran Haider

doi:10.3389/fmicb.2021.699699

Cited by 27 publications

(27 citation statements)

References 32 publications

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“…Besides, microbial genera were the highest at the third sampling stage. In high-throughput sequencing technology, the dominant fungal and bacterial phyla were Ascomycota and Proteobacteria, respectively, which is consistent with the results of a previous study (Huang et al, 2021). The top five fungal genera were Alternaria, Phoma, Symmetrospora, Cladosporium, and Psiloglonium, whereas the top five bacterial genera were Pseudomonas, Pantoea, Kosakonia, Ralstonia, and Sphingomonas; the dominant fungal and bacterial genus were Alternaria and Pseudomonas which were consistent with the result of previous studies (Xu et al, 2014;Liu et al, 2019Liu et al, , 2020.…”

Section: Discussionsupporting

confidence: 91%

“…Moreover, the number of fungal OTUs was subject to change with an increase in disease severity. The α-diversity indices of fungi showed that there were no differences in the healthy group over time at the three leaf positions (Huang et al, 2021 ). A significant difference based on leaf types was observed where the relative abundance of predominant phyla, Ascomycota, and Basidiomycota from leaves infected by A .…”

Section: Discussionmentioning

confidence: 99%

“…Purified cultures were stored at 4°C on PDA slants. Molecular identification was conducted according to the method of Huang et al ( 2021 ). The PCR products were electrophoresed on a 1% agarose gel and the purification of the ITS 500 bp fragment was exercised using Gene JET (Thermo Fisher Scientific, Waltham, MA, United States).…”

Section: Methodsmentioning

confidence: 99%

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Spatio-Temporal Variation in the Phyllospheric Microbial Biodiversity of Alternaria Alternata-Infected Tobacco Foliage

Dai

Wang³

et al. 2022

Front. Microbiol.

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Phyllospheric microbial composition of tobacco (Nicotiana tabacum L.) is contingent upon certain factors, such as the growth stage of the plant, leaf position, and cultivar and its geographical location, which influence, either directly or indirectly, the growth, overall health, and production of the tobacco plant. To better understand the spatiotemporal variation of the community and the divergence of phyllospheric microflora, procured from healthy and diseased tobacco leaves infected by Alternaria alternata, the current study employed microbe culturing, high-throughput technique, and BIOLOG ECO. Microbe culturing resulted in the isolation of 153 culturable fungal isolates belonging to 33 genera and 99 bacterial isolates belonging to 15 genera. High-throughput sequencing revealed that the phyllosphere of tobacco was dominantly colonized by Ascomycota and Proteobacteria, whereas, the most abundant fungal and bacterial genera were Alternaria and Pseudomonas. The relative abundance of Alternaria increased in the upper and middle healthy groups from the first collection time to the third, whereas, the relative abundance of Pseudomonas, Sphingomonas, and Methylobacterium from the same positions increased during gradual leaf aging. Non-metric multi-dimensional scaling (NMDs) showed clustering of fungal communities in healthy samples, while bacterial communities of all diseased and healthy groups were found scattered. FUNGuild analysis, from the first collection stage to the third one in both groups, indicated an increase in the relative abundance of Pathotroph-Saprotroph, Pathotroph-Saprotroph-Symbiotroph, and Pathotroph-Symbiotroph. Inclusive of all samples, as per the PICRUSt analysis, the predominant pathway was metabolism function accounting for 50.03%. The average values of omnilog units (OUs) showed relatively higher utilization rates of carbon sources by the microbial flora of healthy leaves. According to the analysis of genus abundances, leaf growth and leaf position were the important drivers of change in structuring the microbial communities. The current findings revealed the complex ecological dynamics that occur in the phyllospheric microbial communities over the course of a spatiotemporal varying environment with the development of tobacco brown spots, highlighting the importance of community succession.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

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Spatio-Temporal Variation in the Phyllospheric Microbial Biodiversity of Alternaria Alternata-Infected Tobacco Foliage

Dai

Wang³

et al. 2022

Front. Microbiol.

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“…The early bolting of Qianhu has largely been studied, but the role of plant microbiota, particularly rhizosphere microbiota, has not been considered. Didymella is a pathogen of tea leaf spots ( Huang et al., 2021 ). In the present study, this fungus was found to be the different fungus in the pre- and post-bolting stages of Qianhu.…”

Section: Discussionmentioning

confidence: 99%

Fungal isolates influence the quality of Peucedanum praeruptorum Dunn

et al. 2022

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The symbiotic relationship between beneficial microorganisms and plants plays a vital role in natural and agricultural ecosystems. Although Peucedanum praeruptorum Dunn is widely distributed, its development is greatly limited by early bolting. The reason for early bolting in P. praeruptorum remains poorly characterized. We focus on the plant related microorganisms, including endophytes and rhizosphere microorganisms, by combining the traditional isolation and culture method with metagenomic sequencing technology. We found that the OTUs of endophytes and rhizosphere microorganisms showed a positive correlation in the whole growth stage of P. praeruptorum. Meanwhile, the community diversity of endophytic and rhizosphere fungi showed an opposite change trend, and bacteria showed a similar change trend. Besides, the microbial communities differed during the pre- and post-bolting stages of P. praeruptorum. Beneficial bacterial taxa, such as Pseudomonas and Burkholderia, and fungal taxa, such as Didymella and Fusarium, were abundant in the roots in the pre-bolting stage. Further, a strain belonging to Didymella was obtained by traditional culture and was found to contain praeruptorin A, praeruptorin B, praeruptorin E. In addition, we showed that the fungus could affect its effective components when it was inoculated into P. praeruptorum. This work provided a research reference for the similar biological characteristics of perennial one-time flowering plants, such as Saposhnikovia divaricate, Angelica sinensis and Angelica dahurica.

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“…Changes of phyllospheric fungi and bacteria can potentially be used to evaluate the effects of efficient disease management ( Huang et al, 2021a ). With the development of sequencing technology, the number of studies on phyllosphere microorganisms has gradually increased in recent years, creating a new era of phyllosphere microbial community research ( Hassani et al, 2018 ; Fitzpatrick et al, 2020 ; Gong and Xin, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Effect of disease severity on the structure and diversity of the phyllosphere microbial community in tobacco

Sun

Shi²,

Huang³

et al. 2023

Front. Microbiol.

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Tobacco target spot is a serious fungal disease and it is important to study the similarities and differences between fungal and bacterial community under different disease severities to provide guidance for the biological control of tobacco target spot. In this study, tobacco leaves at disease severity level of 1, 5, 7 and 9 (S1, S5, S7, and S9) were collected, both healthy and diseased leaf tissues for each level were sampled. The community structure and diversity of fungi and bacteria in tobacco leaves with different disease severities were compared using high-throughput sequencing technology. The results indicated that there was a significant differences in the community structure of fungi and bacteria for both healthy and diseased samples depending on the disease severity. In both healthy and diseased tobacco leaves for all four different disease severities, the most dominant fungal phylum was Basidiomycota with a high prevalence of genus Thanatephorus. The relative abundance of Thanatephorus was most found at S9 diseased samples. Proteobacteria represent the most prominent bacterial phylum, with Pseudomonas as predominant genus, followed by Pantoea. The relative abundance of Pseudomonas was most found at S7 healthy samples. In fungal community, the Alpha-diversity of healthy samples was higher than that of diseased samples. In contrast, in bacterial community, the Alpha-diversity of healthy samples was lower than that of diseased samples. LEfSe analysis showed that the most enrich fungal biomarker was Thanatephorus cucumeris in diseased samples. Clostridium disporicum and Ralstonia pickettii were the most enrich bacterial biomarker in healthy samples. FUNGuild analysis showed that the pathotroph mode was the most abundant trophic modes. The relative abundance of pathotroph mode in diseased samples changes insignificantly, but a peak at S5 was observed for healthy samples. PICRUSt analysis showed that most bacterial gene sequences seem to be independent of the disease severity. The results of this study provide scientific references for future studies on tobacco phyllosphere microecology aiming at prevention and control of tobacco target spot.

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Phyllospheric Microbial Composition and Diversity of the Tobacco Leaves Infected by Didymella segeticola

Cited by 27 publications

References 32 publications

Spatio-Temporal Variation in the Phyllospheric Microbial Biodiversity of Alternaria Alternata-Infected Tobacco Foliage

Spatio-Temporal Variation in the Phyllospheric Microbial Biodiversity of Alternaria Alternata-Infected Tobacco Foliage

Fungal isolates influence the quality of Peucedanum praeruptorum Dunn

Effect of disease severity on the structure and diversity of the phyllosphere microbial community in tobacco

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