Phylodynamic and genetic diversity of parvoviruses of cats in southern India

Karanam, Bhaswanth; Srinivas, Mouttou Vivek; Vasu, Jayalakshmi; Xavier, Antony Prabhakar; Karuppiah, Rajkumar; Shanmugam, Venkatesa Permal; Mukhopadhyay, H. K.

doi:10.1007/s13337-022-00760-4

Cited by 4 publications

(5 citation statements)

References 25 publications

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“…This infection is highly contagious and closely related to high morbidity and mortality among cat animals [ 1 , 28 , 29 ]. Therefore, timely follow-up, isolation, and monitoring of genetic evolution of FPV field strains are of great importance for further development of prevention and control strategies [ 30 , 31 ].…”

Section: Discussionmentioning

confidence: 99%

Identification and Genome Characterization of Novel Feline Parvovirus Strains Isolated in Shanghai, China

Liu

et al. 2023

CIMB

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Feline panleukopenia virus (FPV) is the causative agent of hemorrhagic gastroenteritis in feline animals. FPV has been evolving over time, and there have been several different strains of the virus identified. Some of these strains may be more virulent or more resistant to current vaccines than others, which highlights the importance of ongoing research and monitoring of FPV evolution. For FPV genetic evolution analysis, many studies focus on the main capsid protein (VP2), but limited information is available on the nonstructural gene NS1 and structural gene VP1. In the present study, we firstly isolated two novel FPV strains circulating in Shanghai, China, and performed full-length genome sequencing for the desired strains. Subsequently, we focused on analyzing the NS1, VP1 gene, and the encoding protein, and conducted a comparative analysis among the worldwide circulating FPV and Canine parvovirus Type 2 (CPV-2) strains, which included the strains isolated in this study. We found that the 2 structural viral proteins, VP1 and VP2, are splice variants, and VP1 has a 143 amino-acid-long N-terminal compared to VP2. Furthermore, phylogenetic analysis showed that divergent evolution between FPV and CPV-2 virus strains were clustered mostly by country and year of detection. In addition, much more continuous antigenic type changes happened in the process of CPV-2 circulating and evolution compared to FPV. These results stress the importance of the continuous study of viral evolution and provide a comprehensive perspective of the association between viral epidemiology and genetic evolution.

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Section: Discussionmentioning

confidence: 99%

Identification and Genome Characterization of Novel Feline Parvovirus Strains Isolated in Shanghai, China

Liu

et al. 2023

CIMB

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“…The condition for the PCR ampli cation was carried out as per Karanam et al [20]. The PCR-ampli ed products were resolved on 1.5% agarose gel in Tris acetate EDTA buffer and visualized under UV transilluminator (Syngene).…”

Section: Pcr Ampli Cationmentioning

confidence: 99%

“…Eighteen randomly selected representative recent samples found positive by primer pair H for /H rev and obtained from diverse locations in India (3 samples from each regions), eleven repository CPV strains (2009-2019 isolates) and a current Indian CPV vaccine strain were subjected to PCR ampli cations to yield seven overlapping PCR products for generation of the whole CPV genome using the seven sets of primers detailed in Supplementary Table 2, followed by custom sequencing and sequence alignment. The PCR reaction mixture and the conditions of the PCR assay to amplify each fragment were carried out as per Karanam et al [20]. The details of the eighteen clinical sample and eleven repository CPV isolates subjected for whole genome sequence analysis are shown in Table 1 and Supplementary Table 1 respectively.…”

Section: Whole Genome Sequencingmentioning

confidence: 99%

“…The phylogenetic tree was constructed along with the corresponding sequences of repository isolates, Indian vaccine strain and the reference CPV strains from various parts of the world with clear background on strain, country and collection date available in NCBI nucleotide database using Neighbor-Joining method implemented in MEGA 11 programme [24]. The con dence level of branching in the phylogenetic tree was evaluated as per Karanam et al [20].…”

Section: Phylogenetic Analysis Of Vp2 and Ns1 Genementioning

confidence: 99%

“…The original prototype CPV-2 is no longer present in canines but is available only in CPV vaccines [15]. Previous studies in India have identi ed the CPV-2a variant as the dominant strain with few reports of CPV-2b variant and CPV-2c [11,12,[16][17][18][19][20]. But few recent studies have reported increased occurrence of CPV-2c in various neighbouring countries like China, Taiwan, Myanmar and Thailand [21][22][23].…”

Section: Introductionmentioning

confidence: 99%

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Whole genome sequence analysis of canine parvovirus reveals pan-Indian replacement with Novel CPV-2c strain

Reddy,

Mukhopadhyay,

Srini

et al. 2024

Preprint

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Canine parvovirus (CPV) infection causes severe gastroenteritis in canines with high mortality in puppies. This virus evolved from FPV by altering its transferrin receptor (TfR) followed by emergence of CPV-2 variants in subsequent years by altering the immunodominant VP2 amino acid residues. Though the previous studies analysed VP2 gene, there are limited studies on non-structural protein (NS1 & NS2) genes. In this study, comparative whole genomic analysis was performed with the CPV suspected clinical samples collected Pan-India in 2023 with the repository CPV strains (2009–2019 isolates) and the current Indian CPV vaccine strain. The present pan-Indian study showed that the CPV-2c (N426E) had almost completely replaced the previously dominant CPV-2a variant (N426) in India. The Q370R mutation of VP2 was the most common change in the recent CPV-2c strain (CPV-2c 370Arg variant). Phylogeny showed the existence of three clades among the recent CPV-2c strains. The study identified several new positive selection sites in VP1 (N terminus), VP2, NS1 and NS2 genes in the recent CPV strains indicating emergence of new CPV-2c variants with varied antigenic and replication properties. The predominant ‘CPV-2c 370Arg variants’ were grouped along with the Chinese and Nigerian CPV-2c strains away from the CPV vaccine strain and repositories. VP2 epitope analysis predicted nine amino acid variations (includes 2 new variations) at four potential linear B-cell epitopes in the ‘CPV-2c 370Arg variants’ underlying chance of vaccine failure. This pan-Indian study, laid the foundation for further research concerning the dynamics of virus evolution and understanding genetic mutations.

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Whole-genome sequence analysis of canine parvovirus reveals replacement with a novel CPV-2c strain throughout India

Reddy,

Srinivas,

Vasu

et al. 2024

Arch Virol

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Phylodynamic and genetic diversity of parvoviruses of cats in southern India

Cited by 4 publications

References 25 publications

Identification and Genome Characterization of Novel Feline Parvovirus Strains Isolated in Shanghai, China

Identification and Genome Characterization of Novel Feline Parvovirus Strains Isolated in Shanghai, China

Whole genome sequence analysis of canine parvovirus reveals pan-Indian replacement with Novel CPV-2c strain

Whole-genome sequence analysis of canine parvovirus reveals replacement with a novel CPV-2c strain throughout India

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