Phylogenesis and regulated expression of the RUNT domain transcription factors RUNX1 and RUNX3

Levanon, Ditsa; Glusman, Gustavo; Bettoun, David; Ben-Asher, Edna; Negreanu, Varda; Bernstein, Yael; Harris-Cerruti, Catherine; Brenner, Ori; Eilam, Raya; Lotem, Joseph; Fainaru, Ofer; Goldenberg, Dalia; Pozner, Amir; Woolf, Eilon; Xiao, Cuiying; Yarmus, Merav; Groner, Yoram

doi:10.1016/s1079-9796(03)00023-8

Cited by 31 publications

(32 citation statements)

References 10 publications

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“…Consistent with our Runx1 expression studies, ectopic expression of Runx3 in gastric cancer cell lines resulted in inhibition of cell growth (Fukamachi and Ito, 2004). Runx1 and Runx3 bind similar sequences and both form protein complexes with the cofactor CBFb; hence, both proteins may inhibit growth by activating expression of common tumor suppressor genes (Levanon et al, 2003). Runx3 mRNA was also detectable in mature macrophages (data not shown) and this expression may be involved in the ability of CBFb expression alone to effect cell growth and survival.…”

Section: Discussionmentioning

confidence: 94%

The Runx1 transcription factor controls CSF-1-dependent and -independent growth and survival of macrophages

et al. 2005

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Gene translocations that repress the function of the Runx1 transcription factor play a critical role in the development of myeloid leukemia. In this report, we demonstrate that Runx1 precisely regulates c-fms (CSF-1 receptor) gene expression. Runx1 controlled expression by binding to multiple sites within the mouse c-fms gene, allowing interaction between promoter and downstream enhancer elements. The runx1 and c-fms genes showed an identical pattern of expression in mature macrophages. Runx1 expression was repressed in CSF-1 stimulated, proliferating bone marrow-derived macrophages (BMM) and significantly increased in quiescent, CSF-1 starved cells. The RAW264.7 and Mono-Mac-6, macrophage-like cell lines expressed low levels of Runx1 and both showed growth arrest and cell death with ectopic expression of Runx1. The EM-3 cell line, which represents an early myeloid progenitor cell line, showed growth arrest with Runx1 expression in the absence of any detectable changes in cell differentiation. These findings suggest that Runx1 regulates growth and survival of myeloid cells and provide a novel insight into the role of Runx family gene translocations in leukemogenesis.

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Section: Discussionmentioning

confidence: 94%

The Runx1 transcription factor controls CSF-1-dependent and -independent growth and survival of macrophages

et al. 2005

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“…An involvement of Runx1 in cranial sensory neuron differentiation is consistent with recent studies showing that Runx1 and the related gene Runx3 are expressed in separate dorsal root ganglion neuron subtypes. Runx1 is expressed in nociceptive neurons whereas Runx3 is found in proprioceptive neurons (12,15,24). Importantly, Runx3 inactivation causes a perturbation of the development of dorsal root ganglion proprioceptive, but not nociceptive, neurons (15,24).…”

Section: Discussionmentioning

confidence: 99%

“…Runx1 is expressed in nociceptive neurons whereas Runx3 is found in proprioceptive neurons (12,15,24). Importantly, Runx3 inactivation causes a perturbation of the development of dorsal root ganglion proprioceptive, but not nociceptive, neurons (15,24). Future studies aimed at further elucidating the involvement of Runx1 in cell survival and͞or differentiation events in selected sensory and motor neurons will provide important information that will help clarify mechanisms underlying the development of neuronal subtypes in the mammalian CNS and PNS.…”

Section: Discussionmentioning

confidence: 99%

AML1/Runx1 is important for the development of hindbrain cholinergic branchiovisceral motor neurons and selected cranial sensory neurons

Theriault

Roy

Stifani

2004

Proc. Natl. Acad. Sci. U.S.A.

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The mechanisms that regulate the acquisition of distinctive neuronal traits in the developing nervous system are poorly defined. It is shown here that the mammalian runt-related gene Runx1 is expressed in selected populations of postmitotic neurons of the embryonic central and peripheral nervous systems. These include cholinergic branchial and visceral motor neurons in the hindbrain, restricted populations of somatic motor neurons of the median and lateral motor columns in the spinal cord, as well as nociceptive and mechanoreceptor neurons in trigeminal and vestibulocochlear ganglia. In mouse embryos lacking Runx1 activity, hindbrain branchiovisceral motor neuron precursors of the cholinergic lineage are correctly specified but then fail to progress to a more differentiated state and undergo increased cell death, resulting in a neuronal loss in the mantle layer. In contrast, the development of cholinergic somatic motor neurons is unaffected. Runx1 inactivation also leads to a loss of selected sensory neurons in trigeminal and vestibulocochlear ganglia. These findings uncover previously unrecognized roles for Runx1 in the regulation of mammalian neuronal subtype development.

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“…IM-9 (human B-lymphoblastoid), Jurkat (human T-lymphocyte), THP-1 (human acute monocytic leukemia), and HL-60 (human acute promyelocytic leukemia) cell lines were cultured in RPMI 1640 medium supplemented with 10% (v/v) fetal bovine serum. Mouse NK cells were isolated from spleen of Runx3-deficient (19) and wild type mice by negative selection (R&D MagCollect mouse NK cell isolation kit, MAGM210) and cultured for 7 days in RPMI 1640 medium containing 10% (v/v) fetal calf serum, 2 mM L-glutamine, 1% sodium pyruvate, 1% nonessential amino acids, 50 M 2-mercaptoethanol, and 1000 units/ml IL2 (Biological Industries, Beit-Haemek, Israel, 30-T209B). All cultures were supplemented with 100 units/ml penicillin, 100 units/ml streptomycin.…”

Section: Methodsmentioning

confidence: 99%

Role of Runt-related Transcription Factor 3 (RUNX3) in Transcription Regulation of Natural Cytotoxicity Receptor 1 (NCR1/NKp46), an Activating Natural Killer (NK) Cell Receptor

Lai

Mager

2012

Journal of Biological Chemistry

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Phylogenesis and regulated expression of the RUNT domain transcription factors RUNX1 and RUNX3

Cited by 31 publications

References 10 publications

The Runx1 transcription factor controls CSF-1-dependent and -independent growth and survival of macrophages

The Runx1 transcription factor controls CSF-1-dependent and -independent growth and survival of macrophages

AML1/Runx1 is important for the development of hindbrain cholinergic branchiovisceral motor neurons and selected cranial sensory neurons

Role of Runt-related Transcription Factor 3 (RUNX3) in Transcription Regulation of Natural Cytotoxicity Receptor 1 (NCR1/NKp46), an Activating Natural Killer (NK) Cell Receptor

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