Phylogenetic affiliation and antimicrobial effects of endophytic actinobacteria associated with medicinal plants: prevalence of polyketide synthase type II in antimicrobial strains

Gohain, Anwesha; Sarma, Rupak K.; Debnath, Rajal; Saikia, Juthika; Sarmah, Ranjan Kumar; Saikia, Ratul

doi:10.1007/s12223-018-00673-0

Cited by 9 publications

(5 citation statements)

References 47 publications

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“…However, the smallest ZOI was noted against P. aeruginosa (Clinical) 9.75±0.71 mm. The results were similar to the findings of an investigation done by Gohain et al, in which most of the endophytic isolates were found to be active against Gram-positive bacteria, while no activity was observed against P. aeruginosa (13) . The pattern of antimicrobial effect of a rare endophytic actinobacteria A. cyanogriseus SIR5 was found to be similar and in agreement with the present investigation (6) .In Gram-negative bacteria, the production of hydrolytic enzymes, especially β -lactamase, and the presence of a lipopolysaccharide (LPS) layer on top of the peptidoglycan cell wall are responsible for reduced vulnerability to antibiotics (6,20) .…”

Section: Secondary Screeningsupporting

confidence: 91%

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Endophytic Streptomyces rochei BpR-2 GER (ON142045) from Bryophyllum pinnatum (Lam.) Oken with Antimicrobial and Antibiofilm Potential

Yadav¹,

Wag²,

Gupta³

2023

IJST

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Objectives: To explore the endophytic actinobacteria from the root tissue of Bryophyllum pinnatum (Lam.) Oken and its assessment for antimicrobial and antibiofilm activity against human pathogens. Methods: Surface sterilized root tissues of B. pinnatum have been seeded in different isolation media, to obtain colonies of endophytic actinobacteria. A polyphasic approach has been used to identify the isolate. Cross streak and disc diffusion methods have been used to perform the antimicrobial activity. However, the crystal violet assay assessed the antibiofilm activity, and its visualization was done under the light and fluorescent microscope. Findings: By polyphasic approach, Actinobacteria strain BpR-2 GER has been confirmed to be the member of genus Streptomyces, further through 16S rRNA gene sequence analysis, the strain has been identified as Streptomyces rochei that has 100% sequence similarity with Streptomyces rochei NRRL B-2410 (MUMD01000370). The isolate has exhibited extensive antagonistic interaction with Clinical and MTCC (Microbial Type Culture Collection) pathogens ranging from 9.75±0.71 to 19.38±1.30 mm. The highest zone of inhibition was recorded against Gram-positive bacteria B. subtilis (MTCC-441) of 19.38±1.30 mm and Gram-negative bacteria Proteus mirabilis (Clinical) of 16.25±0.71 mm. The strain has also exhibited significant antibiofilm potential against the MTCC-7443 (85.13±0.003 to 88.28±0.003%) and Clinical (85.13±0.003 to 88.28±0.003%) strain of Staphylococcus aureus. Novelty: This is the first report of S. rochei BpR-2 GER, a root endophyte of B. pinnatum with broad-spectrum antimicrobial activity and a significant antibiofilm property.

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Section: Secondary Screeningsupporting

confidence: 91%

“…Streptomyces sp. is a dominant genus of soil actinobacteria and has been frequently reported as an endophyte of various plants (13) . Recently, the strain of Streptomyces sp.…”

Section: Isolation Of Endophytic Actinobacteriamentioning

confidence: 99%

Endophytic Streptomyces rochei BpR-2 GER (ON142045) from Bryophyllum pinnatum (Lam.) Oken with Antimicrobial and Antibiofilm Potential

Yadav¹,

Wag²,

Gupta³

2023

IJST

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“…There are many endophytic actinomycetes reported from Emblica officinalis because of it being an important ethnomedical plant of the Indo-Pak region. A recent study by Gohain et al (2019) explored the diversity of the endophytic actinomycetes harboured by the plant. The study observed the majority of the isolates belonged to the genus Streptomyces that is in agreement with the studies conducted earlier.…”

Section: Association Of Endophytic Actinomycetes With Their Host Plantmentioning

confidence: 99%

Indo-Pak Medicinal Plants and Their Endophytes: An Emphasis on Nutraceutical and Bioactive Potential

2021

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The Indo-Pak region has deep historical roots of traditional medicine. In both Pakistan and India, the local population has relied upon the Unani-Tibb (Graeco-Arabic) and Ayurveda medicinal systems for centuries. With the increase in our understanding of ethnobotany and endophytes, the significance of the traditional plants in the region has also increased. The traditional Indo-Pak plants such as Neem and Tulsi have been studied not only for their wide array of pharmaceutical activities but also for their endophytes. Extracts of different parts of neem have been used as antimicrobial, antiviral, antimalarial, anti-carcinogenic, antipyretic, anti-ulcer and anti-inflammatory agents, whereas its seeds are a rich source of proteins and fatty acids. Latest researches have proved the nutraceutical potential of plants such as imli, amla and soanjna in the Indo-Pak region. Further understanding of these plants can lead to unbeatable bioactive and nutraceutical sources. In this chapter, we will focus on the traditional plants of the Indo-Pak region, their endophytes particularly the genus actinomycetes and the recent studies done on their diverse metabolites.

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“…These phytochemicals have been reported to perform different biological activities, e.g. anticancer ( Chander, 2018 ), anti-inflammatory ( Zhang and Tsao, 2016 ), antibacterial ( Gohain et al, 2019 ), antiviral ( Orhan et al, 2012 ), anti-ischemic ( Pozdnyakov et al, 2019 ) and vasodilator ( Luna-Vázquez et al, 2018 ). In addition, they may inhibit lipid peroxidation and platelet aggregation, and increase capillary permeability and fragility ( Smeriglio et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

In vitro examination of anti-parasitic, anti-Alzheimer, insecticidal and cytotoxic potential of Ajuga bracteosa Wallich leaves extracts

Imran

Jan

Faisal

et al. 2021

Saudi Journal of Biological Sciences

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This research study is mainly focused to evaluate the anti-parasitic, insecticidal, cytotoxic and anti-alzheimer potential of various leaf extracts of Ajuga bracteosa Wallich ex Bentham. 04 different extracts were prepared using solvent of different polarity to determine the best candidate for potent bioactivity i.e. n-hexane (NH), Ethyl acetate (EA), Ethanol (EL) and Chloroform (CH). Concentrations of each extracts were made specified for all activities. All extracts were exploited for broad range of biomedical applications including leishmaniasis, in vitro anti-Alzheimer, insecticidal and cytotoxic studies. Our results showed that A. bracteosa n-hexane extract was highly active against Leishmania Tropica with significant inhibition of 58 ± 1.61 for promastigote and 63 ± 2.29 for amastigote at 1000 μg/mL. Furthermore, promising anti-alzheimer activity acetylcholinesterase (AChE) 46 ± 0.83 and butrylcholineterase (BChE) 49 ± 1.17 was noted for n-hexane. The insecticidal potential of these extracts were test against five different insects ( Rhyzopertha dominica, Trogoderma granarium, Tribolium castaneum, Sitophilus oryze, and Callosobruchus analis ). The higest mortality rate of insecticidal activity was recorded by n-hexane followed by Ethyl acetate whereas ethanol extract was found to be less effective against all the test species. Significant cytotoxic potential of each plant sample against Artemia salina thus aware us for further detailed research to find out novel drugs. Based on our results we believe that Ajuga bracteosa could be used to develop as a potential botanical insecticide against different insect and pests, such as aphids as well as an excellent source for the compound isolation as anti-tumor agent.

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Phylogenetic affiliation and antimicrobial effects of endophytic actinobacteria associated with medicinal plants: prevalence of polyketide synthase type II in antimicrobial strains

Cited by 9 publications

References 47 publications

Endophytic Streptomyces rochei BpR-2 GER (ON142045) from Bryophyllum pinnatum (Lam.) Oken with Antimicrobial and Antibiofilm Potential

Endophytic Streptomyces rochei BpR-2 GER (ON142045) from Bryophyllum pinnatum (Lam.) Oken with Antimicrobial and Antibiofilm Potential

Indo-Pak Medicinal Plants and Their Endophytes: An Emphasis on Nutraceutical and Bioactive Potential

In vitro examination of anti-parasitic, anti-Alzheimer, insecticidal and cytotoxic potential of Ajuga bracteosa Wallich leaves extracts

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