Phylogenetic‐affiliation, antimicrobial potential and PKS gene sequence analysis of moderately halophilic <i>Streptomyces</i> sp. inhabiting an Indian saltpan

Jose, Polpass Arul; Santhi, Velayudhan Satheeja; Jebakumar, Solomon Robinson David

doi:10.1002/jobm.201000253

Cited by 29 publications

(20 citation statements)

References 31 publications

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“…In India, there are many coastal and inland solar salterns from which salt is produced for human consumption and industrial needs. Several actinomycetes, found to be proficient to produce antibiotic compounds and halotolerant enzymes, have been reported from the coastal solar salterns (Vasavada et al, 2006; Thumar and Singh, 2009; Thumar et al, 2010; Jose et al, 2011). More recently, diverse actinomycetes have been isolated and there phylogenetic diversity was reported from an Indian coastal solar saltern (Jose and Jebakumar, 2012).…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic appraisal of antagonistic, slow growing actinomycetes isolated from hypersaline inland solar salterns at Sambhar salt Lake, India

Jose¹,

Jebakumar²

2013

Front. Microbiol.

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Inland solar salterns established in the vicinity of Sambhar Lake are extreme saline environments with high salinity and alkalinity. In view of the fact that microbes inhabiting such extreme saline environments flourish the contemporary bioprospecting, it was aimed to selectively isolate slow growing and rare actinomycetes from the unexplored solar salterns. A total of 14 slow growing actinomycetes were selectively isolated from three composite soil samples of inland solar salterns. Among the isolates, four groups were formed according to similarity of the banding patterns obtained by amplified ribosomal DNA restriction analysis (ARDRA). A subset of representative isolates for each ARDRA group was identified using 16S rDNA sequence based phylogenetic analysis and subsequently the entire isolates were assigned under three different genera; Streptomyces, Pseudonocardia, and Actinoalloteichus. The genus Streptomyces was found to be the dominant among the isolates. Furthermore, rare actinomycete genus Actinoalloteichus was isolated for the first time from solar saltern. Determination of salt-tolerance revealed that certain level of salt-tolerance and moderate halophilism occurs among the actinomycetes isolated from the inland salterns. In addition, all the acinomycetes were screened in two levels to unravel their ability to produce antimicrobial compounds. Significant antimicrobial activity was found among the actinomycetes against a range of bacteria and fungi to worth further characterization of these persuasive actinomycetes and their antimicrobial secondary metabolites. In a nutshell, this study offered a first interesting insight on occurrence of antagonistic rare actinomycetes and streptomycetes in inland solar salterns associated with Sambhar salt Lake.

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Section: Introductionmentioning

confidence: 99%

Phylogenetic appraisal of antagonistic, slow growing actinomycetes isolated from hypersaline inland solar salterns at Sambhar salt Lake, India

Jose¹,

Jebakumar²

2013

Front. Microbiol.

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“…The halophilic actinomycetes are noteworthy for their potential to produce bioactive compounds and enzymes [5,6]. In recent years , novel actinomycetes of diverse genera: Nocardiopsis, Saccharopolyspora , Myceligenerans and Streptomyces have been isolated from hypersaline environments [7-11]. Despite these findings, little is known about the diversity of actinomycetes in hypersaline environments.…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic diversity of actinomycetes cultured from coastal multipond solar saltern in Tuticorin, India

Jose

Jebakumar

2012

Aquat Biosyst

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BackgroundHypersaline solar salterns are extreme environments in many tropical and subtropical regions throughout the world. In India, there are several coastal solar salterns along with the coastal line of the Bay of Bengal and Arabian Sea and inland solar salterns around Sambhar saltlake, from which sodium chloride is obtained for human consumption and industrial needs. Studies on characterization of such coastal and inland solar salterns are scarce and both the bacterial and archaeal diversity of these extreme saline environment remains poorly understood. Moreover, there are no reports on exclusive diversity of actinomycetes inhabiting Indian solar salterns.ResultsSoil sediments were collected from both concentrator and crystallizer ponds of solar salterns and subjected to detailed physico-chemical analysis. Actinomycetes were selectively isolated by employing selective processing methods and agar media. A total of 12 representatives were selected from the 69 actinomycete isolates obtained from the saltern soil samples, using Amplified Ribosomal DNA Restriction Analysis. Sequencing and analysis of 16S rDNA from chosen representative isolates displayed the presence of members affiliated to actinobacterial genera: Streptomyces, Micromonospora, Nocardia, Nocardiopsis, Saccharopolyspora and Nonomuraea. The genus Streptomyces was found to be the dominant among the isolates. Furthermore, rare actinomycete genus Nonomuraea was isolated for the first time from Indian solar salterns.ConclusionsTo the best of our knowledge, this study constitutes the first characterization of actinomycete diversity centred on solar salterns located in the eastern coastal region of India. Furthermore, this is the very first report of isolation of Nonomuraea species from solar salterns and also from India. As actinomycetes encompass recurrently foremost sources of biotechnologically important member of the microbial communities, the actinomycetes retrieved from the Indian saltern soil samples laid the platform to search for novel biotechnologically significant bioactive substances.

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“…The Streptomyces sp. JAJ06 has previously been identified as seawater dependent antibiotic producing actinomycete [5]. Undefined nature of seawater can affect the reproducibility of the production profile of actinomycetes [6].…”

Section: Discussionmentioning

confidence: 99%

“…JAJ06 was previously isolated from a coastal solar saltern soil and extensively studied for their antibacterial secondary metabolite [5]. The strain was maintained over the surface of ISP4 agar slants supplemented with 3% of sea salt (w/v).…”

Section: Methodsmentioning

confidence: 99%

“…JAJ06 is a Gram-positive, moderately halophilic Streptomyces strain, which has previously been isolated from hypersaline coastal solar saltern at Tuticorin, India [4]. This strain produces an antimicrobial polyketide compound which has been reported to have potent antimicrobial activity against a set of bacteria and yeast with significant minimal inhibitory concentrations [5]. Production of antibiotic compound in JAJ06 has been reported to have seawater dependence.…”

Section: Introductionmentioning

confidence: 99%

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Formulation and Statistical Optimization of Culture Medium for Improved Production of Antimicrobial Compound byStreptomycessp. JAJ06

Jose

Sivakala

Jebakumar

2013

International Journal of Microbiology

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Streptomyces sp. JAJ06 is a seawater-dependent antibiotic producer, previously isolated and characterised from an Indian coastal solar saltern. This paper reports replacement of seawater with a defined salt formulation in production medium and subsequent statistical media optimization to ensure consistent as well as improved antibiotic production by Streptomyces sp. JAJ06. This strain was observed to be proficient to produce antibiotic compound with incorporation of chemically defined sodium-chloride-based salt formulation instead of seawater into the production medium. Plackett-Burman design experiment was applied, and three media constituents, starch, KBr, and CaCO3, were recognised to have significant effect on the antibiotic production of Streptomyces JAJ06 at their individual levels. Subsequently, Response surface methodology with Box-Behnken design was employed to optimize these influencing medium constituents for the improved antibiotic production of Streptomyces sp. JAJ06. A total of 17 experiments were conducted towards the construction of a quadratic model and a second-order polynomial equation. Optimum levels of medium constituents were obtained by analysis of the model and numerical optimization method. When the strain JAJ06 was cultivated in the optimized medium, the antibiotic activity was increased to 173.3 U/mL, 26.8% increase as compared to the original (136.7 U/mL). This study found a useful way to cultivate Streptomyces sp. JAJ06 for enhanced production of antibiotic compound.

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Phylogenetic‐affiliation, antimicrobial potential and PKS gene sequence analysis of moderately halophilic Streptomyces sp. inhabiting an Indian saltpan

Cited by 29 publications

References 31 publications

Phylogenetic appraisal of antagonistic, slow growing actinomycetes isolated from hypersaline inland solar salterns at Sambhar salt Lake, India

Phylogenetic appraisal of antagonistic, slow growing actinomycetes isolated from hypersaline inland solar salterns at Sambhar salt Lake, India

Phylogenetic diversity of actinomycetes cultured from coastal multipond solar saltern in Tuticorin, India

Formulation and Statistical Optimization of Culture Medium for Improved Production of Antimicrobial Compound byStreptomycessp. JAJ06

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