Phylogenetic analyses of the Fusarium poae, Fusarium sporotrichioides and Fusarium langsethiae species complex based on partial sequences of the translation elongation factor-1 alpha gene

Phylogenetic analyses and toxigenic profiles of Fusarium equiseti and Fusarium acuminatum isolated from cereals from Southern Europe

“…Additionally, these techniques have provided the basis necessary for developing rapid, specific and accurate diagnostic methods based on PCR. These can be used to predict mycotoxin risk, providing the information necessary for early control strategies to be adopted (Jurado et al, , 2006bKnutsen et al, 2004;Konstantinova and Yli-Mattila, 2004). Several genomic sequences have been used to analyse intraspecific variability in Fusarium, including intron regions of histone coding genes, the P-tubulin gene ((STUB), the calmodulin gene (O'Donnell et al, 1998a;Steenkamp et al, 2002), and the translation elongation factor gene EF-\a (O'Donnell et al, 1998b(O'Donnell et al, , 2000.…”

Novel Methods for the Quantification of Pathogenic Fungi in Crop Plants: Quantitative PCR and ELISA Accurately Determine Fusarium Biomass

“…The extensive application of DNA based identification technologies has increased the knowledge on suitable diagnostic DNA fragments of Fusarium species such as ITS (internal transcribes spacer) or IGS (intergenic spacer) sequences (Gagkaeva & Yli-Mattila, 2004, Jurado et al, 2006, Konstantinova & Yli-Mattila, 2004, Kulik 2008), mitochondrial DNA (Laday et al, 2004), the -tubulin encoding gene , the translation elongation factor gene (Knutsen et al, 2004) and the calmodulin gene (Mule et al, 2004) which were sequenced from numerous Fusarium spp. As a result highly specific PCR primers could be developed for Fusarium detection.…”

Molecular Tools for Detection of Plant Pathogenic Fungi and Fungicide Resistance

“…Other housekeeping genes with higher variability are being more extensively used to develop diagnostics for fungi, including nuclear genes such as -tubulin (Aroca et al, 2008;Fraaije et al, 2001;Mostert et al, 2006), translation elongation factor 1 alpha (TEF 1 (Geiser et al, 2004;Knutsen et al, 2004, Kristensen et al, 2005, calmodulin (Mulè et al, 2004), avirulence genes (Lievens et al, 2009), and mitochondrial genes such as the multicopy cox I and cox II and their intergenic region (Martin & Tooley, 2003;Nguyen & Seifert, 2008;Seifert et al, 2007). Mating type genes also show high diversity and fast evolutionary rate and could be used for inter-and intra-species differentiation, e.g.…”