Phylogenetic analysis and genetic diversity of 3′ region of rtxA gene from geographically diverse strains of Moraxella bovis, Moraxella bovoculi and Moraxella ovis

Farias, Luana D’Avila; Maboni, Grazieli; Matter, Letícia Beatriz; Scherer, Charles Fernando Capinos; Libardoni, Felipe; Vargas, Águeda Castagna de

doi:10.1016/j.vetmic.2015.05.025

Cited by 10 publications

(2 citation statements)

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“…Over the last 50 years, numerous epidemiological observations and experimental challenge models have implicated Moraxella bovis as the major causal agent of IBK (Gould et al, 2013;O'Connor et al, 2012). A major virulence factor of Moraxella bovis is a secreted cytotoxin shown to reproduce IBK lesions in calves (Angelos et al, 2007;Farias et al, 2015). Moraxella bovoculi, a newly described species has been isolated and characterized in association with IBK in the absence of Moraxella bovis (Loy and Brodersen, 2014).…”

Section: Introductionmentioning

confidence: 99%

A multiplex real-time PCR assay for the detection and differentiation of five bovine pinkeye pathogens

Zheng

Porter

Noll

et al. 2019

Journal of Microbiological Methods

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A B S T R A C TInfectious bovine keratoconjunctivitis (IBK), also known as pinkeye, is one of the most common eye diseases in cattle. Several pathogens have been associated with IBK cases, however, Moraxella bovis, Moraxella bovoculi, Mycoplasma bovis, Mycoplasma bovoculi and bovine herpesvirus type 1 (BHV-1) are most frequently observed. A multiplex real-time PCR assay using two reactions was developed for the detection and differentiation of these five pathogens. Detection sensitivities of the multiplex assays were compared to singleplex reactions testing for the same targets. Correlation coefficients (R 2 ) of > 0.99, and PCR efficiencies between 92 and 106% were demonstrated in all singleplex and multiplex real-time PCR reactions. The limits of detection (LOD) of multiplex assays for Moraxella bovis, Moraxella bovoculi, Mycoplasma bovis, Mycoplasma bovoculi and BHV-1 were 19, 23, 25, 24 and 26 copies per reaction, respectively. No cross amplification was observed for specificity testing of 179 IBK positive clinical samples and 55 non-target clinical samples. Percentage of clinical samples positive for Mycoplasma bovoculi, Moraxella bovoculi, Moraxella bovis, BHV-1 and Mycoplasma bovis were 88.8% (159/179), 75.9% (136/179), 60.3% (108/179), 11.7% (21/179) and 10.0% (18/179), respectively. Moraxella bovis, Moraxella bovoculi and Mycoplasma bovoculi were more prevalent than Mycoplasma bovis and BHV-1 in IBK samples collected from animals in this study population. Our data indicates that the multiplex real-time PCR panel assay is highly sensitive and highly specific for the detection and differentiation of the five major pathogens associated with bovine pinkeye. Fig. 3. The prevalence of the five pathogens in IBK samples. Panel A: Prevalence of the five pathogens from the 179 bovine ocular swab samples that were subjected to multiplex real-time PCR testing. Panel B: Co-infections among the three most prevalent IBK pathogens in the 179 positive clinical samples: 95 (53.0%) samples were positive for both Moraxella bovis and Moraxella bovoculi; 100 (55.8%) samples were positive for both Moraxella bovis and Mycoplasma bovoculi; 129 (72.0%) samples were positive for both Moraxella bovoculi and Mycoplasma bovoculi and 93 (51.9%) samples were positive for Moraxella bovis, Moraxella bovoculi and Mycoplasma bovoculi. W. Zheng, et al.

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Section: Introductionmentioning

confidence: 99%

A multiplex real-time PCR assay for the detection and differentiation of five bovine pinkeye pathogens

Zheng

Porter

Noll

et al. 2019

Journal of Microbiological Methods

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“…Conceição et al (2004) showed low correspondence between these two parameters, for M. bovis. However, most recent studies involving the sequencing of cytotoxin from M. bovis, M. bovoculi, and M ovis showed genetic variability in the aminoacid sequence for this virulence factor, depending on the period of isolation (Farias et al, 2015). Moreover, for M. catharralis, the head domains of UspA2/2H promotes a general evasion of the host immune system throughout the extensive sequence polymorphism in this protein (Su et al, 2013).…”

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Genetic differences among Moraxella bovis and Moraxella bovoculi isolates from infectious bovine keratoconjunctivitis (IBK) outbreaks in southern Brazil

et al. 2020

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The objective of this study was to evaluate the genetic diversity of Moraxella bovis and Moraxella bovoculi bacteria isolated from infectious bovine keratoconjunctivitis (IBK) outbreaks in the state of Rio Grande do Sul, Brazil. The genetic diversity among Moraxella spp. was evaluated by RAPD-PCR, JWP1-JWOPA07-PCR, ERIC-PCR and by sequencing the 16S-23S intergenic regions. Based on the dendrogram, two genetically differentiated clades were observed; 14 isolates were classified as M. bovis and 17 as M. bovoculi. Genetic distances between the M. bovis samples ranged from 0.0379 to 0.4285, while for M. bovoculi the dissimilarities ranged from zero to 0.7297. Alternatively, based on sequencing analyses of the 16S-23S intergenic region, M. bovis and M. bovoculi isolates were grouped into the same two different clades, but it was not possible to differentiate between isolates within clades. PCR techniques were demonstrated to be a satisfactory tool to unravel the genetic variability among Moraxella spp., while sequencing of the 16S-23S intergenic region was only able to differentiate two species of the Moraxella genus. Despite sampling geographically close regions, we demonstrate considerable genetic diversity in M. bovis and M. bovoculi strains and genetically distinct M. bovis strains co-infecting the same animal.

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Moraxella

Angelos¹

2022

Pathogenesis of Bacterial Infections in Animals

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Phylogenetic analysis and genetic diversity of 3′ region of rtxA gene from geographically diverse strains of Moraxella bovis, Moraxella bovoculi and Moraxella ovis

Cited by 10 publications

References 19 publications

A multiplex real-time PCR assay for the detection and differentiation of five bovine pinkeye pathogens

A multiplex real-time PCR assay for the detection and differentiation of five bovine pinkeye pathogens

Genetic differences among Moraxella bovis and Moraxella bovoculi isolates from infectious bovine keratoconjunctivitis (IBK) outbreaks in southern Brazil

Moraxella

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