Phylogenetic Analysis and Heterologous Expression of Surface Layer Protein SlpC of<i>Bacillus sphaericus</i>C3-41

Hu, Xiaomin; Li, Jia; Hansen, Benni Winding; Yuan, Zhiming

doi:10.1271/bbb.70747

Cited by 16 publications

(12 citation statements)

References 22 publications

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“…In contrast, the identities were only 24-25% to L. sphaericus nonpathogenic strain CCM2177 (16S rDNA group 4) and 14-15% to the L. sphaericus belonging to the 16S rDNA group 3 (ATCC 14577, JGA-12, NCTC9602). These results are in concordance with the high-identity in amino acid sequences among translated gene sequences in the S-layer protein pathogenic strains reported by Hu et al (2008).…”

Section: Resultssupporting

confidence: 92%

“…In contrast, Hu et al (2008) tested the toxicity of the S-layer protein of L. sphaericus C3-41 and found no significant activity toward C. quinquefasciatus larvae at 80 lg protein/ml. The S-layer protein of the reference strain L. sphaericus 2362 caused 20% mortality in C. quinquefasciatus larvae at 30 lg/ml.…”

Section: Larvicidal Activity Of the S-layer Proteinmentioning

confidence: 96%

“…Bowditch et al (1989) cloned a DNA fragment containing the gene for the S-layer glycoprotein of L. sphaericus 2362 but the S-layer protein showed no toxicity against Culex pipiens larvae. The purified protein extracted from L. sphaericus C3-41 (a mosquito-pathogenic isolate), showed no significant mortality in Culex quinquefasciatus (Hu et al 2008).…”

Section: S-layers Have Been Found In Insect Pathogenicmentioning

confidence: 99%

“…Overnight cultures were centrifuged and the pellets were washed in cold 50 mM Tris/HCl (pH 7.4); the cells were broken by sonication (Hu et al 2008), washed three times and treated with 0.5% Triton X-100 for 10 min at 20°C. The S-layer protein was extracted using guanidine hydrochloride (Gu.HCl) (5 M in 50 mM Tris/HCl buffer, pH 7.4) for 4 h at 4°C.…”

Section: Isolation Of the S-layer Proteinmentioning

confidence: 99%

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Lysinibacillus sphaericus S-layer protein toxicity against Culex quinquefasciatus

2011

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The main toxicity mechanism of Lysinibacillus sphaericus, which is used in the control of mosquitoes, is its binary toxin produced during sporulation; additionally the Mtx1, Mtx2 and Mtx 3 toxins are expressed in vegetative cells. Mosquito larvicidal potency of the S-layer protein that is expressed in vegetative cells has been determined. The protein is similar to other S-layer proteins of mosquitocidal L. sphaericus strains. The LC50 values of the S-layer protein of the L. sphaericus OT4b25, OT4b26, and III(3)7 strains against third-instar larvae of Culex quinquefasciatus were 8.7, 24 and 0.68 μg/ml, respectively. To our knowledge this is the first study showing the mosquito larvicidal potency of the S-layer protein from Lysinibacillus sphaericus.

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Section: Resultssupporting

confidence: 92%

Section: Larvicidal Activity Of the S-layer Proteinmentioning

confidence: 96%

Section: S-layers Have Been Found In Insect Pathogenicmentioning

confidence: 99%

Section: Isolation Of the S-layer Proteinmentioning

confidence: 99%

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Lysinibacillus sphaericus S-layer protein toxicity against Culex quinquefasciatus

2011

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“…However, crystals or inclusions were not observed from either isolate by both phase contrast and transmission electron microscopy (data not shown). Therefore, proteins produced from C32 and M413 during sporulation could be soluble or other noncrystal proteins such as surface layer (Pena et al, 2006;Hu et al, 2008) or spore coat (Bailey-Smith et al, 2005;Johnson et al, 2006) proteins. Identification of these two major proteins along with other minor ones is under evaluation using high-performance liquid chromatography with tandem mass spectrometry detection (LC-MS/ MS) (Golovko and Murphy, 2008).…”

Section: Introductionmentioning

confidence: 99%

Identification of two mosquitocidal Bacillus cereus strains showing different host ranges

Park¹,

Hayes²,

Stout³

et al. 2009

Journal of Invertebrate Pathology

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Comparative analysis for three different immobilisation strategies in the hexavalent chromium biosorption process using Bacillus sphaericus S‐layer

et al. 2011

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Hexavalent chromium constitutes an important water pollutant due to its cancerogenic properties. In countries such as Colombia it is widely used as a preservative in the leather industry. Bacillus sphaericus S-layer exhibits high metal-binding capacity hence the use of this microorganism to remove metals such as chromium from polluted regions based on immobilised cells systems is suggested to be an interesting proposal. The paper describes thermodynamic adsorption process of Cr(VI) on immobilised biomass of B. sphaericus cells on polyurethane foam, sol-gel, and sawdust. The adsorption isotherms describe different immobilisation mechanisms regarding the support utilised. For example, polyurethane and sawdust immobilisation process were described with the classical Langmuir model while sol-gel followed Generalised Freundlich-Kiselev behaviour. Moreover, through determining breakthrough curves in packed columns with each medium we found surface diffusion and mass bulk transfer rate as crucial factors when scaling-up the process.Le chrome hexavalent est un polluant important de l'eau en raison de ses propriétés cancérigènes. Dans des pays tels que la Colombie, le chrome est largement utilisé comme conservateur dans l'industrie du cuir. La couche de surface de Bacillus sphaericus montre de fortes capacités de fixation des métaux, d'où la suggestion de l'intérêt potentiel de l'utilisation de ce micro-organisme pouréliminer des métaux comme le chrome de régions polluées en se basant sur des systèmes cellulaires immobilisés. L'article décrit le processus d'adsorption thermodynamique du Cr(VI) dans une biomasse immobilisée de cellules de B. sphaericus, sur mousse de polyuréthane, avec la méthode sol-gel et sur de la sciure de bois. Les isothermes d'adsorption décrivent différents mécanismes d'immobilisation en fonction du support utilisé. Par exemple, les processus d'immobilisation avec la mousse de polyuréthane et la sciureétaient décrits avec le modèle classique de Langmuir tandis que la méthode sol-gel suivait un comportement Freundlich-Kiselev généralisé. De plus, avec la détermation des courbes d'interférence des colonnesà remplissage pour chaque milieu, nous avons conclu que la diffusion en surface et que le taux de transfert de masseétaient des facteurs cruciaux pour faire passer le processusà une plus grandé echelle.

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Phylogenetic Analysis and Heterologous Expression of Surface Layer Protein SlpC ofBacillus sphaericusC3-41

Cited by 16 publications

References 22 publications

Lysinibacillus sphaericus S-layer protein toxicity against Culex quinquefasciatus

Lysinibacillus sphaericus S-layer protein toxicity against Culex quinquefasciatus

Identification of two mosquitocidal Bacillus cereus strains showing different host ranges

Comparative analysis for three different immobilisation strategies in the hexavalent chromium biosorption process using Bacillus sphaericus S‐layer

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