Phylogenetic analysis of classical swine fever virus isolated from Taiwan

The phylogenetic analysis of 11 CSFV isolates from Karnataka, India obtained during the year 2012-13 was undertaken to obtain the most reliable genetic typing of the CSFV isolates based on E2, NS5B and 5 0 UTR genomic regions. The study indicated that all the 11 CSFV isolates belonged to subgroup 2.2. The most reliable classification was obtained with sequence data from the NS5B region which separated all the isolates based on the history of outbreak and geographic origin. Analysis of full length E2 amino acid sequences revealed different genetic makeup of Indian 2.2 isolates compared to 2.2 isolates from different countries. The group 2.2 viruses are gradually spreading as confirmed by frequent detection/ isolation of group 2.2 viruses in the recent years and replacing the subgroup 1.1 viruses, which were hitherto predominantly involved in CSF outbreaks in India.Keywords Classical swine fever virus Á Phylogenetic analysis Á E2 Á NS5B and 5 0 UTR

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“…In Thailand, subgroup 2.2 became predominant from 1996 onwards. In Taiwan, there has been a switch from subgroups 3.4 to 2.1 after 1996 [5].…”

Section: Discussionmentioning

confidence: 99%

Genetic clustering of recent classical swine fever virus isolates from Karnataka, India revealed the emergence of subtype 2.2 replacing subtype 1.1

et al. 2015

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“…Furthermore, outbreaks of CSFV are often associated with high economic losses due to national movement restrictions and international trade implications. Recently, it has been suggested that there is a change in subgenotype from the historical group 1 or 3 to genogroup 2 (Deng et al 2005;Cha et al 2007). Currently used vaccine strains belong almost exclusively to genogroup 1.…”

Section: Dear Readermentioning

confidence: 99%

Recurring highly contagious virus infections in pigs

Opriessnig

2014

Veterinary Quarterly

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“…In Taiwan, by analyzing the E2 sequences of CSFV from field outbreaks during 1993–2001, CSFVs have been classified into two subgroups – 3.4 and 2.1 [3,4]. Also, the Taiwanese strains of 2.1 were further divided into two different genotypes termed 2.1a and 2.1b which might be introduced from different origins [4].…”

Section: Introductionmentioning

confidence: 99%

Genome and Molecular Characterization of a CSFV Strain Isolated from a CSF Outbreak in South China

et al. 2013

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In the present study, the full-length nucleotide sequences of the CSFV-GZ-2009 strain of classical swine fever virus (CSFV) isolated from a hog pen in Guangdong province in China was determined. Results demonstrated that the genome of CSFV-GZ-2009 is 12,298 nucleotides (nt) in length, is composed of a 373-nt 5′-untranslated region (UTR), has an 11,697-nt open reading frame encoding a polyprotein of 3,898 amino acids, and has a 228-nt 3′-UTR. Genome comparison of the CSFV-GZ-2009 isolate (GenBank accession No. HQ380231) with other CSFV strains was also analyzed. Gene regions from CSFV-GZ-2009 and other known strains were shown to share 92.7–96.7% identity at the nucleotide level and 94.7–99.2% identity at the amino acid level. Phylogenetic analysis of the full-length genome and the following regions E^rns, E2 and NS5B revealed that the CSFV-GZ-2009 isolate was classified within subgroup 1.1 of group I and closely related to the highly virulent strain JL1 (06), cF114, Shimen and SWH with pairwise distances of 0.0037, 0.0043, 0.0058 and 0.0107, respectively. Analysis of recombination with the SimPlot program demonstrated that strain CSFV-GZ-2009 was not a naturally homologous recombinant. Furthermore, the change of clinical signs of pigs after infection of CSFV-GZ-2009 isolates showed typical symptoms such as diarrhea, persistent fever, and mononuclear lymphocytopenia after CSFV infection. Based on phylogenetic analysis and an animal infection test, we could conclude that the CSFV-GZ-2009 isolate belonged to subgroup 1.1 of group I and was of high virulence.

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Phylogenetic analysis of classical swine fever virus isolated from Taiwan

Cited by 72 publications

References 18 publications

Genetic clustering of recent classical swine fever virus isolates from Karnataka, India revealed the emergence of subtype 2.2 replacing subtype 1.1

Genetic clustering of recent classical swine fever virus isolates from Karnataka, India revealed the emergence of subtype 2.2 replacing subtype 1.1

Recurring highly contagious virus infections in pigs

Genome and Molecular Characterization of a CSFV Strain Isolated from a CSF Outbreak in South China

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