Phylogenetic Analysis of Dipterocarps Using Random Amplified Polymorphic DNA Markers

Confirmation of relationships among Boesenbergia (Zingiberaceae) and related genera by RAPD

“…The DNA did not give any band for visualization after RAPD-PCR, possibly because the presence of the phenolic compound that intercalates irreversibly with the DNA helices as reported in the case of DNA from another group of tropical plants (Rajaseger et al, 1997;Rath et al, 1998). The extraction of high quality DNA was optimized by re-extracting the DNA using CTAB DNA extraction protocol and phenol:chloroform:isoamyl alcohol extraction instead of chloroform: isoamyl alcohol extraction.…”

Genetic diversity analysis of Croton antisyphiliticus Mart. using AFLP molecular markers

“…It has been reported that it is difficult to obtain intact DNA from tropical species because of the presence of secondary composites, particularly those in the phenolics class (Rath et al, 1998;Deshmukh et al, 2007). During the C. antisyphiliticus DNA extraction process, both viscosity and oxidation were observed in the samples, and this problem was solved using the protocol developed by Deshmukh et al (2007).…”

Genetic diversity of <i>Persea bombycina</i> from goalpara district of Assam, India

“…The simplicity, efficiency, and relative ease of performing RAPD techniques, without sequence information, have led to their expanded use for taxonomic and systemic analysis and phylogenetic studies of plants, species differentiation, and phylogeographic variation, as well as for studying breeding and genetic relationships [10][11][12][13][14][15][16][17][18][19][20]. RAPD techniques have recently been used in genetic diversity studies of a wide range of tree plants [21,22].…”