Phylogenetic analysis of gastric and enterohepatic Helicobacter species based on partial HSP60 gene sequences

Mikkonen, T.; Kärenlampi, R.; Hänninen, Marja‐Liisa

doi:10.1099/00207713-55-3-1393

Cited by 22 publications

(29 citation statements)

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“…A 515 bp sequence (GenBank accession no. EF070344) obtained from isolate M50 T using the methodology described by Mikkonen et al (2004) placed this taxon into the cluster of H. felis, H. bizzozeronii, H. salomonis and H. cynogastricus, confirming the results based on 16S rRNA gene and ureAB sequence analysis. Only 70-75 % similarity was obtained with these species, yielding sufficient difference to consign isolate M50…”

Section: Strain M50supporting

confidence: 72%

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Helicobacter baculiformis sp. nov., isolated from feline stomach mucosa

Baele

Decostere

Vandamme

et al. 2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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A Gram-negative, microaerophilic slender rod, measuring approximately 10 mm long and approximately 1 mm wide, isolated from the gastric mucosa of a cat and designated strain M50T , was subjected to a polyphasic taxonomic study. Despite its apparent lack of helical coils, the organism showed a corkscrew-like motion by means of multiple sheathed flagella located at both ends of the cell and by a periplasmic fibril coiled around the body. Several highly related Helicobacter species have been isolated from the stomachs of dogs and cats. Helicobacter felis was isolated for the first time from the stomach of a cat and was subsequently also isolated from canine gastric mucosa (Lee et al., 1988). Two other species have been described after their isolation from dog stomachs, namely Helicobacter bizzozeronii and Helicobacter salomonis (Hänninen et al., 1996; Jalava et al., 1997). The prevalence of H. bizzozeronii, H. felis and H. salomonis in the stomachs of 110 dogs and 43 cats was investigated by a multiplex PCR and it was found that in cats, H. felis (63 %) and H. bizzozeronii (35 %) were the most predominant species, while only 2.3 % of the animals were colonized by H. salomonis (Van den Bulck et al., 2005). Based on 16S rRNA gene sequence analysis, all three species are phylogenetically very highly related to each other and to 'Helicobacter heilmannii' type 2 organisms, detected in the human gastric mucosa (Solnick et al., 1993). The similarity of the ureAB urease genes of the three species is, however, lower than 85 %, allowing discrimination between these species. A fourth uncultured species has been detected in wild feline and in human gastric biopsies by PCR. Sequencing analysis of urease genes showed sufficient differences to those of H. felis, H. bizzozeronii and H. salomonis and this fourth uncultured species was provisionally named 'Candidatus Helicobacter heilmannii' (O'Rourke et al., 2004). Recently, Abbreviations: PAGE, polyacrylamide gel electrophoresis; TEM, transmission electron microscopy.The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA, partial ureAB and hsp60 gene sequences of H. baculiformis M50 T are EF070342, EF070343 and EF070344, respectively. Supplementary tables showing the distance matrix values for theHelicobacter strains examined in this study based on 16S rRNA, ureAB and hsp60 gene sequences are available with the online version of this paper.

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Section: Strain M50supporting

confidence: 72%

“…A tree was constructed using the neighbour-joining method and is shown in Fig. 2 Conserved partial 60 kDa heat-shock protein (HSP60) gene sequences have also been shown to give additional phylogenetic information that is useful for differentiating Helicobacter species (Mikkonen et al, 2004). A 515 bp sequence (GenBank accession no.…”

Section: Strain M50mentioning

confidence: 99%

Helicobacter baculiformis sp. nov., isolated from feline stomach mucosa

Baele

Decostere

Vandamme

et al. 2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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“…For sequencing studies, DNA was isolated as described elsewhere (Hänninen et al, 1996(Hänninen et al, , 2003Mikkonen et al, 2004). The nearly complete (at least 1400 bases sequenced) 16S rRNA genes of eight Finnish canine and feline flexispira strains were amplified by PCR with universal primer pair F19-38 (59-CTGG-CTCAGGAYGAACGCTG-39) and R1541-1522 (59-AAGGAGGTG-ATCCAGCCGCA-39).…”

Section: Methodsmentioning

confidence: 99%

Extension of the species Helicobacter bilis to include the reference strains of Helicobacter sp. flexispira taxa 2, 3 and 8 and Finnish canine and feline flexispira strains

Hänninen

Kärenlampi

Koort

et al. 2005

International Journal of Systematic and Evolutionary Microbiology

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The evolution and taxonomy of enterohepatic Helicobacter species with flexispira morphology were studied by a polyphasic approach including phenotypic characterization, analysis of 16S rRNA and ureB gene sequences and dot-blot DNA–DNA hybridization of whole genomic DNA. In addition, available phylogenetic data on the HSP60 gene were used in the analysis. The study included 14 Finnish canine and feline flexispira strains, the reference strains of Helicobacter sp. flexispira taxa 2, 3 and 8 and Helicobacter bilis ATCC 51630T. Phenotypically, all canine and feline strains were similar to H. bilis. Analysis of 16S rRNA gene sequences of these strains revealed a similarity of 97–99·5 %. Similarity of ureB nucleotide and amino acid sequences within the studied flexispira group was 97–100 % and 99–100 %, respectively, revealing close relatedness. ureB sequences of Helicobacter hepaticus had only 64–66 % similarity to the flexispira group. The similarity to Helicobacter trogontum was 81·5–82·1 %. High levels of DNA–DNA hybridization between the strains were found in dot-blot tests. Polyphasic analysis of the phenotypic and genotypic characteristics of the Finnish flexispira strains and the reference strains of taxa 2, 3 and 8 showed that they differed from other Helicobacter species and are members of the previously described species H. bilis. In addition, canine strain F56 differed in all phylogenetic analyses from the H. bilis group and probably represents a novel Helicobacter species.

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“…in different clusters, as described elsewhere (Dewhirst et al, 2005). However, the cladogram obtained from 60 kDa heat-shock protein (HSP60), referred as better marker for Helicobacter species phylogeny (Mikkonen et al, 2004) was similar to the one obtained with 16S rRNA (data not shown). A surprising result was the capacity of the genomic methylation and of MCRM algorithm to cluster separately gastric Helicobacter spp.…”

Section: Discussionmentioning

confidence: 82%

Genomic Methylation Status for Discrimination Among Helicobacter Species: A Bioinformatics Approach

Vale¹,

Vítor²

2008

J Proteomics Bioinform

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The genus Helicobacter comprises several species of both gastric and enterohepatic intestinal bacteria. H. pylori, the type species of the genus, is associated with gastritis, peptic ulcer and gastric cancer in humans. H. pylori genome has a high number of restriction and modification (R-M) systems and their diversity is useful for strain typing. To analyse if such a high number of expressed methyltransferases is a characteristic of the genus Helicobacter, the genomic methylation of five non-pylori Helicobacter spp. (H. canadensis, H. canis, H. felis, H. mustelae and H. pullorum) was determined. The results revealed that the number of R-M systems among nonpylori Helicobacter spp. is smaller than those observed among a group of 221 H. pylori strains (p<0,001), but is greater than those observed for the mean of all bacteria sequenced genomes (p=0,005). 16S ribosomal RNA analysis of H. pylori sequenced strains and five non-pylori Helicobacter spp. clearly isolate H. pylori species. Surprisingly, the analysis of the genomic methylation status by MCRM algorithm performs similarly. This suggests that R-M systems do not appear to be spread in a miscellaneous manner, once even that these genes may be subjected to acquisition and loss; their expression still allows discriminating among Helicobacter spp.

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Phylogenetic analysis of gastric and enterohepatic Helicobacter species based on partial HSP60 gene sequences

Cited by 22 publications

References 0 publications

Helicobacter baculiformis sp. nov., isolated from feline stomach mucosa

Helicobacter baculiformis sp. nov., isolated from feline stomach mucosa

Extension of the species Helicobacter bilis to include the reference strains of Helicobacter sp. flexispira taxa 2, 3 and 8 and Finnish canine and feline flexispira strains

Genomic Methylation Status for Discrimination Among Helicobacter Species: A Bioinformatics Approach

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