Phylogenetic analysis of genus Marinilabilia and related bacteria based on the amino acid sequences of GyrB and emended description of Marinilabilia salmonicolor with Marinilabilia agarovorans as its subjective synonym

Abstract

The detailed phylogenetic relationships for genus Marinilabilia and related taxa were analysed by using DNA gyrase B subunit gene (gyrB) sequences. Anaerobic bacteria in the Cytophaga-Flavobacterium-Bacteroides phylum, na me1 y genera Marinilabilia, Bact eroides, Rikenella, Pre votella and Porphyromonas and C'ophaga fermentans, were clustered in the same branch and the facultative anaerobes Marinilabilia and C'ophaga fermentans formed a subcluster in the branch of the anaerobic bacteria. Phylogenetic analysis using 16s rDNA sequences gave a similar result but with a lower bootstrap value for each cluster. The gyrB sequences of Marinilabilia salmonicolor and Marinilabilia agaroworans were the same, and the relatedness of their chromosomal DNA, as determined by DNA-DNA hybridization, was greater than 70%. These genetic aspects led to the conclusion that M. salmonicolor I F 0 15948T and M. agaroworans IF0 14957T belong to a single species. Since M. salmonicolor was described first, as C'ophaga salmonicolor, M. salmonicolor is a senior subjective synonym of M. agarovorans. Therefore, the name M. salmonicolor should be retained and strain IF0 14957T should be reclassified as M. salmonicolor. However, the agar-degrading ability of strain IF0 14957T is a prominent biochemical characteristic. It is therefore proposed that strain IF0 14957T should be renamed M. salmonicolor biovar agarovorans.

Distribution of two triamines, spermidine and homospermidine, and an aromatic amine, 2-phenylethylamine, within the phylum Bacteroidetes

“…Only a trace amount of spermidine was detected in Porphyromonas levii (Hamana et al, 1995). Spermidine was the major polyamine in Bacteroides splanchnicus and Bacteroides putredinis and Cytophaga xylanolytica, Marinilabilia salmonicolor (Nakagawa and Yamasato, 1996;Suzuki et al, 1999) and Cytophaga fermentans contained spermidine as well.…”

“…The class Bacteroides (order Bacteroidales) containing 13 genera is phylogenetically divided into the four families Prevotellaceae, Porphyromonadaceae, Bacteroidaceae and Rikenellaceae (Boone and Castenholz, 2001;Miyamoto and Itoh, 2000;Nakagawa and Yamasato, 1996;NCBI Home page, 2004;Paster et al, 1994;Suzuki et al, 1999;Weller et al, 2000). Cellular polyamines of 19 additional species were newly analyzed in the present study to display complete polyamine distribution profiles within the total 41 species (47 strains) of the class Bacteroides.…”

Anaerocella delicata gen. nov., sp. nov., a strictly anaerobic bacterium in the phylum Bacteroidetes isolated from a methanogenic reactor of cattle farms

“…Most of strictly anaerobic species in the four families (Bacteroidaceae, Rikenellaceae , Porphyromonadaceae and Prevotellaceae ) other than Marinilabiliaceae (Suzuki et al, 1999) in the order Bacteroidales have been isolated from human or ani-J. Gen. Appl.…”

Labilibacter aurantiacus gen. nov., sp. nov., isolated from sea squirt (Styela clava) and reclassification of Saccharicrinis marinus as Labilibacter marinus comb. nov.

“…Species in the genera Alkaliflexus, Alkalitalea, Anaerophaga, Mangroviflexus, Natronoflexus and Thermophagus are anaerobic, while species in the genera Geofilum, Marinilabilia, Carboxylicivirga and Saccharicrinis are capable of fermentative metabolism. Members of the family Marinilabiliaceae are Gram-stain-negative rods and contain MK-7 as the respiratory quinone [2][3][4][5][6][7][8][9][10][11][12].…”