Phylogenetic analysis of human metapneumovirus detected in hospitalized patients in Kuwait during the years 2009–2011

Al-Turab, Mariam; Chehadeh, Wassim; Al‐Nakib, W.

doi:10.1016/j.jiph.2015.01.008

Cited by 5 publications

(7 citation statements)

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“…Taking into account the different periods of enrollment after symptom onset, there was also no significant correlation observed between viral load and TSSS in these periods (Additional file 5). When the patients' TSSS was grouped into three categories with scores that range from 1 to 8,[9][10][11][12][13][14][15][16], and 17-24 to reflect the relative symptom severity of mild, moderate, and severe, respectively [20] (Fig. 2b), no significant differences in viral load between the three categories of symptom severity were observed.…”

Section: The Impact Of Viral Load On Symptom Severitymentioning

confidence: 99%

“…In contrast, some studies reported that genotype B infection was one of the risk factor for severe disease [5] with more pathological signs on chest X-ray compared with genotype A infection [6]. Furthermore, some associations remain debatable as several studies found no direct correlation between HMPV genotypes and severity of illness [8][9][10]. On the other hand, apart from viral genetic diversity, HMPV viral load was recognized as a risk factor associated with more severe disease outcome leading to hospitalization [11,12].…”

Section: Introductionmentioning

confidence: 99%

“…Although HMPV genetic diversity continues to be described and linked with disease severity in hospitalized children [6], pediatrics [13], elderly adults [10] and immunocompromised patients [3], reports that address this association have been limited in the adult outpatient settings. A recent study which showed that HMPV can also cause respiratory outbreaks in adults [14] highlights the fact that adults may play a role in the transmission and evolutionary dynamics of the virus, and more severe disease could occur in adults during an outbreak.…”

Section: Introductionmentioning

confidence: 99%

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The role of human Metapneumovirus genetic diversity and nasopharyngeal viral load on symptom severity in adults

Oong

Chook

et al. 2018

Virol J

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BackgroundHuman metapneumovirus (HMPV) is established as one of the causative agents of respiratory tract infections. To date, there are limited reports that describe the effect of HMPV genotypes and/or viral load on disease pathogenesis in adults. This study aims to determine the role of HMPV genetic diversity and nasopharyngeal viral load on symptom severity in outpatient adults with acute respiratory tract infections.MethodsSeverity of common cold symptoms of patients from a teaching hospital was assessed by a four-category scale and summed to obtain the total symptom severity score (TSSS). Association between the fusion and glycoprotein genes diversity, viral load (quantified using an improved RT-qPCR assay), and symptom severity were analyzed using bivariate and linear regression analyses.ResultsAmong 81/3706 HMPV-positive patients, there were no significant differences in terms of demographics, number of days elapsed between symptom onset and clinic visit, respiratory symptoms manifestation and severity between different HMPV genotypes/sub-lineages. Surprisingly, elderly patients (≥65 years old) had lower severity of symptoms (indicated by TSSS) than young and middle age adults (p = 0.008). Nasopharyngeal viral load did not correlate with nor predict symptom severity of HMPV infection. Interestingly, at 3–5 days after symptom onset, genotype A-infected patients had higher viral load compared to genotype B (4.4 vs. 3.3 log10 RNA copies/μl) (p = 0.003).ConclusionsOverall, HMPV genetic diversity and viral load did not impact symptom severity in adults with acute respiratory tract infections. Differences in viral load dynamics over time between genotypes may have important implications on viral transmission.Electronic supplementary materialThe online version of this article (10.1186/s12985-018-1005-8) contains supplementary material, which is available to authorized users.

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Section: The Impact Of Viral Load On Symptom Severitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The role of human Metapneumovirus genetic diversity and nasopharyngeal viral load on symptom severity in adults

Oong

Chook

et al. 2018

Virol J

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“…Genotype Reference Sequence Selection: We performed an extensive literature review of articles describing HMPV molecular epidemiology and registered 327 HMPV sequences used as genotype references in 22 different articles; 19 of these articles focused on genotype assignment using the HMPV G gene. Subsequently, we selected sequences that have been used as genotype reference by several authors or sequences of newly identified genotypes for viruses with partial G gene duplications [ 16 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 ]. As our aim focused on genotype assignment using the HMPV G gene ectodomain, sequences with at least the complete ectodomain were selected as references.…”

Section: Methodsmentioning

confidence: 99%

Global Extension and Predominance of Human Metapneumovirus A2 Genotype with Partial G Gene Duplication

Muñoz‐Escalante

Mata-Moreno

Rivera-Alfaro

et al. 2022

Viruses

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Human metapneumovirus (HMPV) is an important respiratory pathogen and is divided in two main groups (A and B). HMPV strains with partial duplications (111-nt and 180-nt duplication) of the G gene have been reported in recent years. Since the initial reports, viruses with these characteristics have been reported in several countries. We analyzed all complete HMPV G gene ectodomain sequences available at GenBank to determine if viruses with 111-nt or 180-nt duplication have become the leading HMPV strains worldwide, and to describe their temporal and geographic distribution. We identified 1462 sequences that fulfilled study criteria (764 HMPV A and 698 HMPV B) reported from 37 countries. The most frequent HMPV A genotype was A2b2 (n = 366), and the most frequent B genotype was B2 (n = 374). A total of 84 sequences contained the 111-nt duplication, and 90 sequences contained the 180-nt duplication. Since 2016, viruses with a partial duplication comprise the most frequent HMPV A sequences globally and have displaced other HMPV A viruses in Asia, Europe, and South America; no sequences of viruses with partial duplication have been reported in North America or Africa so far. Continued surveillance of HMPV is required to identify the emergence and spread of epidemiologically relevant variants.

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“…Moreover, recent phylogenetic and evolutionary analyses of F and G genes collected in a 10-year period revealed that the A2 lineage could be separated into two other lineages, A2a and A2b, defining a total of 5 HMPV genetic lineages (8). Another recent study found that HMPV strains of the B genotype clustered separately from strains of the known B1 and B2 lineages, suggesting the existence of a third B lineage (9). confirmed that HMPV infections caused a significant fraction (ϳ5 to 20%) of ARTIs that were previously considered to have an unknown cause.…”

Section: Introductionmentioning

confidence: 99%

Modulation of Host Immunity by the Human Metapneumovirus

et al. 2016

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SUMMARYGlobally, as a leading agent of acute respiratory tract infections in children <5 years of age and the elderly, the human metapneumovirus (HMPV) has gained considerable attention. As inferred from studies comparing vaccinated and experimentally infected mice, the acquired immune response elicited by this pathogen fails to efficiently clear the virus from the airways, which leads to an exaggerated inflammatory response and lung damage. Furthermore, after disease resolution, there is a poor development of T and B cell immunological memory, which is believed to promote reinfections and viral spread in the community. In this article, we discuss the molecular mechanisms that shape the interactions of HMPV with host tissues that lead to pulmonary pathology and to the development of adaptive immunity that fails to protect against natural infections by this virus.

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Phylogenetic analysis of human metapneumovirus detected in hospitalized patients in Kuwait during the years 2009–2011

Abstract: Our data showed the co-circulation of hMPV genotypes A2b and B2 in Kuwait with genetic diversity suggestive of evolution through negative selection.

Cited by 5 publications

References 43 publications

The role of human Metapneumovirus genetic diversity and nasopharyngeal viral load on symptom severity in adults

The role of human Metapneumovirus genetic diversity and nasopharyngeal viral load on symptom severity in adults

Global Extension and Predominance of Human Metapneumovirus A2 Genotype with Partial G Gene Duplication

Modulation of Host Immunity by the Human Metapneumovirus

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