Phylogenetic analysis of Hungarian goose parvovirus isolates and vaccine strains

Abstract

Polymerase chain reaction and sequencing were used to analyse goose parvovirus field isolates and vaccine strains. Two fragments of the genome were amplified. Fragment "A" represents a region of VP3 gene, while fragment "B" represents a region upstream of the VP3 gene, encompassing part of the VP1 gene. In the region of fragment "A" the deduced amino acid sequence of the strains was identical, therefore differentiation among strains could be done only at the nucleotide level, which resulted in the formation of three groups: Hungarian, West-European and Asian strains. In the region of fragment "B", separation of groups could be done by both nucleotide and deduced amino acid sequence level. The nucleotide sequences resulted in the same groups as for fragment "A" but with a different clustering pattern among the Hungarian strains. Within the "Hungarian" group most of the recent field isolates fell into one cluster, very closely related or identical to each other, indicating a very slow evolutionary change. The attenuated strains and field isolates from 1979/80 formed a separate cluster. When vaccine strains and field isolates were compared, two specific amino acid differences were found that can be considered as possible markers for vaccinal strains. Sequence analysis of fragment "B" seems to be a suitable method for differentiation of attenuated vaccine strains from virulent strains.

Coronaviruses in poultry and other birds

“…Geese have also been the subject of study with respect to parvovirus (Tatár-kis et al ., 2004), goose haemorrhagic polyoma virus (Lacroux et al ., 2004), reovirus infections and West Nile virus (Banet-Noach et al ., 2003) infections.…”

Identification of sequence changes in live attenuated goose parvovirus vaccine strains developed in Asia and Europe

“…The strain VG32/1 was purchased from Impfstoffwerk Dessau-Tornau GmbH (Rodleben, Germany). VG32/1 possesses the K75N mutation in its VP1 sequence, which is a genetic marker of European vaccine strains (Tatár-Kis et al, 2004). DNA was extracted from 200 ml allantoic fluid or reconstituted vaccine using a DNeasy tissue kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions.…”

Evidence for natural recombination in the capsid gene VP2 of Taiwanese goose parvovirus

“…Originally, prototypical MDPV and GPV strains were characterized mainly by sequence analysis of the capsidencoding regions in the genome, and they showed low levels of nucleotide sequence diversity and genetic variability in the field [18][19][20]. Over the last two decades, however, genetically different strains of MDPV and GPV were isolated and identified from birds with classical Muscovy duck parvovirosis and Derzsy's disease, respectively [8,10].…”