Phylogenetic analysis of intestinal bacteria of freshwater salmon<i>Salmo salar</i>and sea trout<i>Salmo trutta trutta</i>and diet

Skrodenytė-Arbačiauskienė, Vesta; Sruoga, Aniolas; Butkauskas, Dalius; Skrupskelis, Kęstutis

doi:10.1111/j.1444-2906.2008.01656.x

Cited by 55 publications

(32 citation statements)

References 36 publications

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“…So the association of several bacteria in the intestinal tract of milkfish have the ability to defend itself against disease. This results were similar with several other studies that has been published [27,28,29,30]. The criteria of bacterial probiotics candidate should has the ability to inhibit pathogenic bacteria and able to compete with pathogenic bacteria in the intestinal tract.…”

Section: Bacterial Sensitivity Testsupporting

confidence: 94%

The Diversity of Gut Bacteria Associated with Milkfish (Chanos Chanos Forsksal) from Northern Coast of Central Java, Indonesia

Prayitno

Sarwan²,

Sarjito

2015

Procedia Environmental Sciences

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A total of 30 bacterial isolates associated with milkfish gut were collected from traditional ponds at Northern Coast of Central Java, Indonesia. The study was conducted to isolate and identify the milkfish gut bacteria, and evaluate their potential for probiotics. Based on rapid grouping by using rep-PCR resulted in six groups. The six group represented by isolates of BS 11, BPI 01, BPL 10, BPi 03; BPi 08 and BPL 06 were then further selected for subsequent DNA sequencings. According to DNA sequencing it was demonstrated that the diversity of bacteria associated with milfish gut collected from Northern Coast of Central Java, revealed closely similar to Shewanella upenei, Basillus sp, Vibrio fluvialis, Shewanella algae, Shewanella sp. and Photobacterium ganghwense. Further sensitivity test among six baterial isolates demonstrated that Shewanella upenei and Shewanella algae were able to prevent the growth of Vibrio fluvialis and Photobacterium ganghwense. The six selected isolates were also successfully demonstrated antibacterial activity against pathogenic bacteria Vibrio alginoliticus and Vibrio parahaemolitycus. From this study, it can be concluded that the six bacteria isolated from milkfish intestine were potentially developed for probiotics.

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Section: Bacterial Sensitivity Testsupporting

confidence: 94%

The Diversity of Gut Bacteria Associated with Milkfish (Chanos Chanos Forsksal) from Northern Coast of Central Java, Indonesia

Prayitno

Sarwan²,

Sarjito

2015

Procedia Environmental Sciences

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“…; Bacanu and Oprea ), but results from studies that compared several fish species collected from the same area showed a marked specificity between fish species and microbiota (Skrodenyte‐Arbaciauskiene et al . ; Smriga et al . ; Larsen et al .…”

Section: Discussionmentioning

confidence: 99%

Characterization of the gut microbiota of three commercially valuable warmwater fish species

2014

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Aims: Due to the strong influence of the gut microbiota on fish health, dominant bacterial species in the gut are strong candidates for probiotics. This study aimed to characterize the gut microbiota of channel catfish Ictalurus punctatus, largemouth bass Micropterus salmoides and bluegill Lepomis macrochirus to provide a baseline for future probiotic studies. Methods and Results:The gut microbiota of five pooled individuals from each fish species was identified using 16S rRNA pyrosequencing. Microbiota differed significantly between fish species in terms of bacterial species evenness. However, all gut communities analysed were dominated by the phylum Fusobacteria, specifically the species Cetobacterium somerae. Relatively high abundances of the human pathogens Plesiomonas shigelloides and Fusobacterium mortiferum, as well as members of the genus Aeromonas, suggest these species are normal inhabitants of the gut. Conclusions: The overwhelming dominance of the genus Cetobacterium in all species warrants further investigation into its role in the fish gut microbiota. Significance and Impact of the Study: This study provides the first characterization of the gut microbiota of three economically significant fishes and establishes a baseline for future probiotic trials.

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“…Only about 1% of all gut bacteria in fish can be cultured, including Enterobacteriaceae , Pseudomonas spp., Staphylococcus spp., Acinetobacter spp., Aeromonas spp., Vibrio spp. and Bacillus spp., which severely limited early assessments of fish intestinal microbiota (Merrifield et al, 2009), with a limited number of species cultivated from zebrafish (Cantas et al, 2012) and trout (Skrodenyté-Arbačiauskiené et al, 2008). In Atlantic salmon limited species richness was found by TTGE (Navarrete et al, 2009a) and DGGE (Reveco et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Environmental and physiological factors shape the gut microbiota of Atlantic salmon parr (Salmo salar L.)

2017

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Gut microbes are key players in host immune system priming, protection and development, as well as providing nutrients to the host that would be otherwise unavailable. Due to this importance, studies investigating the link between host and microbe are being initiated in farmed fish. The establishment, maintenance and subsequent changes of the intestinal microbiota are central to define fish physiology and nutrition in the future. In fish, unlike mammals, acquiring intestinal microbes is believed to occur around the time of first feeding mainly from the water surrounding them and their microbial composition over time is shaped therefore by their habitat.Here we compare the distal intestine microbiota of Atlantic salmon parr reared in a recirculating laboratory aquarium with that of age matched parr maintained in cage culture in an open freshwater loch environment of a commercial fish farm to establish the microbial profiles in the gut at the freshwater stage and investigate if there is a stable subset of bacteria present regardless of habitat type. We used deep sequencing across two variable regions of the 16S rRNA gene, with a mean read depth of 180,144 ± 12,096 raw sequences per sample. All individual fish used in this study had a minimum of 30,000 quality controlled reads, corresponding to an average of 342 ± 19 Operational Taxonomic Units (OTUs) per sample, which predominantly mapped to the phyla Firmicutes, Proteobacteria, and Tenericutes.The results indicate that species richness is comparable between both treatment groups, however, significant differences were found in the compositions of the gut microbiota between the rearing groups. Furthermore, a core microbiota of 19 OTUs was identified, shared by all samples regardless of treatment group, mainly consisting of members of the phyla Proteobacteria, Bacteroidetes and Firmicutes. Core microbiotas of the individual rearing groups were determined (aquarium fish: 19 + 4 (total 23) OTUs, loch fish: 19 + 13 (total 32) OTUs), indicating that microbe acquisition or loss is occurring differently in the two habitats, but also that selective forces are acting within the host, offering niches to specific bacterial taxa.The new information gathered in this study by the Illumina MiSeq approach will be useful to understand and define the gut microbiota of healthy Atlantic salmon in freshwater and expand on previous studies using DGGE, TGGE and T-RFPL. Monitoring deviations from these profiles, especially the core microbes which are present regardless of habitat type, might be used in the future as early indicator for intestinal health issues caused by sub optimal feed or infectious diseases in the farm setting.Statement of relevanceThe Microbiome is central to gut health, local immune function and nutrient up take. We have used deep sequencing approach to show differences in rearing conditions of Atlantic salmon. This work is of interest to aquaculture nutritionists.

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Phylogenetic analysis of intestinal bacteria of freshwater salmonSalmo salarand sea troutSalmo trutta truttaand diet

Cited by 55 publications

References 36 publications

The Diversity of Gut Bacteria Associated with Milkfish (Chanos Chanos Forsksal) from Northern Coast of Central Java, Indonesia

The Diversity of Gut Bacteria Associated with Milkfish (Chanos Chanos Forsksal) from Northern Coast of Central Java, Indonesia

Characterization of the gut microbiota of three commercially valuable warmwater fish species

Environmental and physiological factors shape the gut microbiota of Atlantic salmon parr (Salmo salar L.)

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