Phylogenetic analysis of Japanese <i>Armillaria</i> based on the intergenic spacer (IGS) sequences of their ribosomal DNA

Terashima, Kazuhisa; Y., J.; Yajima, Takashi; Igarashi, Tsuneo; Miura, Kiyoshi

doi:10.1111/j.1439-0329.1998.tb01161.x

Cited by 33 publications

(21 citation statements)

References 28 publications

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“…The mycelia were harvested and frozen in liquid nitrogen, then freeze-dried. PCR-RFLP analysis The template DNA for PCR amplification was prepared using the modified method of Volk et al (1996) described in our previous report (Terashima et al, 1998). PCR-RFLP analysis was carried out using the technique of Harrington and Wingfield (1995).…”

Section: Methodsmentioning

confidence: 99%

Identification of Armillaria species from Hokkaido by analysis of the intergenic spacer (IGS) region of ribosomal DNA using PCR-RFLP

et al. 1998

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The intergenic spacer (IGS) region, which is located between the 3' end of 26S ribosomal DNA (rDNA) and the 5' end of 5S rDNA, of six Armillaria species from Hokkaido was investigated using polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP). Restriction with only Alu I could distinguish A. mellea subsp, nipponica from the other species. With Alu I and Dde I, A. ostoyae and A. gallica could be distinguished from the other species. Digestion with Alu I resulted in two patterns (types A and B) of A. singula and three patterns (types A, B, and C) of A.[ezoensis. One pattern (type B) of the former species and two patterns (types B and C) of the latter species were each different from those of the other species. Armillaria sinapina gave only one Alu I digestion pattern, which was identical to that of A. jezoensis (type A) and A. singula (type A). However, by digestion with Dde I, A. singula (type A) could be distinguished from A. jezoensis (type A) and A. sinapina.

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Section: Methodsmentioning

confidence: 99%

Identification of Armillaria species from Hokkaido by analysis of the intergenic spacer (IGS) region of ribosomal DNA using PCR-RFLP

et al. 1998

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“…With the exception of a few phylogenetic studies that have focused on the species occurring in Japan (Terashima et al 1998;Hasegawa et al 2010;Ota et al 2012), nothing was previously known regarding the phylogeny of Chinese Armillaria species prior to this study. This study also expanded the current IGS-1 and TEF-1 DNA sequence database for Armillaria species and the data can now be employed in future research to identify field isolates from China using sequence comparisons.…”

Section: Phylogeny Of Armillaria Species From Chinamentioning

confidence: 99%

“…For phylogenetic inference, the internally transcribed spacer regions (ITS) and intergenic spacer region one (IGS-1) have been useful in studies focused on the relationships of taxa from Africa (Coetzee et al 2005a), South America (Pildain et al 2009), Australasia (Coetzee et al 2001), North America (Anderson and Stasovski 1992), Europe (Chillali et al 1998) and Asia (Terashima et al 1998;Coetzee et al 2000). In addition, sequences for part of the transcription elongation factor one alpha (TEF-1) gene has been used to determine the phylogenetic relationships of taxa from Japan (Hasegawa et al 2010), Europe (Tsykun et al 2013) and a global collection of isolates of Armillaria species (Maphosa et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic relationships among biological species of Armillaria from China

et al. 2015

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“…12,[15][16][17] PCR-RFLP analysis profiles of intergenic spacer 1 (IGS-1) have been successfully applied to the Armillaria species identification. 12,13,18,19) Sequencing of the internal transcribed spacer (ITS) and intergenic spacer 1 (IGS-1) regions 14,20,21) has also been used for identification. The ITS and IGS sequences available in the GenBank and the development of efficient software have provided a valuable tool for differentiation as well as for the detection of polymorphism and heterogeneity among species.…”

Section: 2)mentioning

confidence: 99%

Identification of Armillaria nabsnona in Gastrodia Tubers

Sekizaki

Kuninaga

Yamamoto

et al. 2008

Biological & Pharmaceutical Bulletin

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Armillaria species (Basidiomycota, Agaricales, Tricholomataceae) are well known to be the cause of root rot and death to woody plants in boreal, temperate and tropical regions worldwide.1) Generally this species has a broad variety of hosts, presenting pathogenic or symbiotic properties depending on the available substrate. 1,2)Armillaria has been identified as an associate in certain achlorophyllous taxa of Orchidaceae including Gastrodia elata BL. 3,4) and Galeola septentrionalis REICHB. 5) Although many reports refer to Armillaria mellea as the main fungus symbiont of G. elata, 3,6) 4)In eastern traditional medicine, the tubers of this orchid have been used for the treatment of many kinds of diseases including headache, rheumatism, seizure, dizziness and circulation problems. 7,8) G. elata is considered as a difficult species to cultivate due to the need for different kinds of fungi colonization to achieve its full development.6) To increase the knowledge of the cultivation process and understand its relationship with the Armillaria, it is important to first identify which Armillaria species is involved in the association.In past decades, the identification of Armillaria spp. was based on mating tests and morphological characteristics. 2,9,10) However, those methods are very limited due to the great number of biological species and similar morphological characteristics among them.11,12) Furthermore, mating pairings require fresh haploid cultures that sometimes are not available and a long period is required to obtain results. 13,14) Methods based on molecular techniques are nowadays routinely used and provided a new perspective for phylogenetic and taxonomic studies.12,15-17) PCR-RFLP analysis profiles of intergenic spacer 1 (IGS-1) have been successfully applied to the Armillaria species identification. 12,13,18,19) Sequencing of the internal transcribed spacer (ITS) and intergenic spacer 1 (IGS-1) regions 14,20,21) has also been used for identification. The ITS and IGS sequences available in the GenBank and the development of efficient software have provided a valuable tool for differentiation as well as for the detection of polymorphism and heterogeneity among species. The ITS and IGS-1 of the ribosomal DNA are known to be highly polymorphic enabling the accurate species differentiation of the Armillaria.11) In some cases, however, ITS1 region sequences were proven to be nearly uniform among the Armillaria of northern hemisphere species. 11)The aim of this study was to apply molecular biological tools to identify which Armillaria species are involved in the association with G. elata. The ITS and IGS-1 regions of rDNA were amplified and sequenced for further comparison with other Armillaria species. PCR-RFLP of the IGS-1 region was performed with three different endonucleases. Morphological characteristics and compatibility tests were also used in the analysis. MATERIALS AND METHODS Fungal Isolation and CultivationFungal samples were harvested in July 2005, found in different root parts of G. elata, which ...

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Phylogenetic analysis of Japanese Armillaria based on the intergenic spacer (IGS) sequences of their ribosomal DNA

Cited by 33 publications

References 28 publications

Identification of Armillaria species from Hokkaido by analysis of the intergenic spacer (IGS) region of ribosomal DNA using PCR-RFLP

Identification of Armillaria species from Hokkaido by analysis of the intergenic spacer (IGS) region of ribosomal DNA using PCR-RFLP

Phylogenetic relationships among biological species of Armillaria from China

Identification of Armillaria nabsnona in Gastrodia Tubers

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