Phylogenetic analysis of partial VP7 gene of the emerging human group A rotavirus G12 strains circulating in Tunisia

Moussa, Amal; Fredj, M. Ben; BenHamida-Rebaï, Meriam; Fodha, I.; Boujaafar, N.; Trabelsi, Abdelhalim

doi:10.1099/jmm.0.000420

Cited by 10 publications

(31 citation statements)

References 53 publications

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“…Moreover, G12P [8] and G12P [6] were the most prevalent genotypes being responsible for over 43 % of all rotavirus infections in Ivorian children [38,53]. In the MENA region, G12 was reported in Saudi Arabia (7 %) [10], Yemen (2 %) [11], Iraq (7 %) [12], Iran (9.4 %) [13], Egypt (9.2 %) [16], Tunisia (5.2 %) [14,15], Pakistan (12.85 %) [17,54] and Turkey (15.6 %) [18]. In Lebanon, G12 accounted for 1.6 % of all rotavirus infections [21], where the sole genotype detected was G12P [6].…”

Section: Discussionmentioning

confidence: 99%

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The origins of G12P[6] rotavirus strains detected in Lebanon

Reslan

Mishra

Finianos

et al. 2021

Journal of General Virology

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The G12 rotaviruses are an increasingly important cause of severe diarrhoea in infants and young children worldwide. Seven human G12P[6] rotavirus strains were detected in stool samples from children hospitalized with gastroenteritis in Lebanon during a 2011–2013 surveillance study. Complete genomes of these strains were sequenced using VirCapSeq-VERT, a capture-based high-throughput viral-sequencing method, and further characterized based on phylogenetic analyses with global RVA and vaccine strains. Based on the complete genomic analysis, all Lebanese G12 strains were found to have Wa-like genetic backbone G12-P[6]-I1-R1-C1-M1-A1-N1-T1-E1-H1. Phylogenetically, these strains fell into two clusters where one of them might have emerged from Southeast Asian strains and the second one seems to have a mixed backbone between North American and Southeast Asian strains. Further analysis of these strains revealed high antigenic variability compared to available vaccine strains. To our knowledge, this is the first report on the complete genome-based characterization of G12P[6] emerging in Lebanon. Additional studies will provide important insights into the evolutionary dynamics of G12 rotaviruses spreading in Asia.

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Section: Discussionmentioning

confidence: 99%

“…They have emerged predominantly in combination with either P [6] or P [8], and less commonly with P [4] or P [9] genotype. In the Middle East and North Africa (MENA), G12 strains have been reported in several countries; Saudi Arabia [10], Yemen [11], Iraq [12], Iran [13], Tunisia [14,15], Egypt [16], Pakistan [17] and Turkey [18].…”

Section: Introductionmentioning

confidence: 99%

The origins of G12P[6] rotavirus strains detected in Lebanon

Reslan

Mishra

Finianos

et al. 2021

Journal of General Virology

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“…Based on epidemiological data and phylogenetic analysis, G12 strains are subdivided into four lineages (I–IV) 38 . Sequence analysis of VP7 gene showed that all HP G12 RVAs clustered within the modern lineage‐III, indicating the derivation of these strains from a common origin.…”

Section: Discussionmentioning

confidence: 99%

Phylogenetic analysis of VP7 and VP4 genes of the most predominant human group A rotavirus G12 identified in children with acute gastroenteritis in Himachal Pradesh, India during 2013–2016

Gupta

Gauhar

Bubber

et al. 2021

Journal of Medical Virology

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G12 strains are now considered to be the sixth most prevalent human rotaviruses globally. India has introduced rotavirus vaccine Rotavac® into the national immunization program in 2016 and Himachal Pradesh (HP) is the first state to launch it. During epidemiological rotavirus surveillance in HP, predominance of G12 rotaviruses was observed. This study investigated the genetic variability and evolution of HP G12 strains (n = 15) associated with P-genotypes P[6], P[4], and P[8] identified between 2013 and 2016. Phylogenetic analysis of VP7 gene revealed that all characterized G12 strains clustered in lineage-III and diversified into three subclusters indicating that these strains may have originated from three different ancestral G12 strains. The comparative sequence analysis of HP strains with Rotavac® and Rotarix® vaccine strains revealed various amino acid substitutions in epitope regions of VP7 and VP4 proteins especially at the antibody neutralization sites. Only 12/29 VP7 epitope residues and 2/25 VP4 epitope residues were found to be conserved between HP rotavirus strains and vaccine strains. Both long and short electropherotypes were observed in G12P[4] strains, while a single long electropherotype was observed in G12P[6] strains. Children of ≤11 months were significantly infected with G12 rotaviruses. The frequency of vomiting episodes (≥5/ day) was significantly higher in children infected with G12 rotavirus strains as compared to non-G12 rotaviruses (p = 0.0405). Our study provides the comprehensive data on clinical characteristics and evolutionary pattern of the G12 rotavirus, the most prevalent strain in HP and emphasizes the need to monitor these strains for inclusion in future vaccine.

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“…G5, G8 and G10 have been identified recently in different parts of the world, including India, Ghana, Australia, Argentina, Paraguay and Malawi (Hoshino, Jones, Ross, & Kapikian, ). Recently, the G12 genotypes have been re‐emerged in humans in the United States (Wylie, Stanley, Tekippe, Mihindukulasuriya, & Storch, ), Spain (Cilla, Montes, & Arana, ) and Tunisia (Moussa et al, ). The remarkable diversity of human RVA strains is associated with three major evolutionary mechanisms: the accumulation of point mutations leading to antigenic drift; reassortment of cognate genome segments promoting antigenic shift; and the reassortment of genes in strains found in animals that could introduce new antigen types into humans (Martella, Bányai, Matthijnssens, Buonavoglia, & Ciarlet, ; Matthijnssens et al, ).…”

Section: Introductionmentioning

confidence: 99%

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confidence: 99%

Molecular characterization of group A rotavirus from rhesus macaques ( Macaca mulatta ) at human–wildlife interfaces in Bangladesh

Islam

Hossain

Haider

et al. 2019

Transbound Emerg Dis

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Group A rotavirus (RVA) is an important cause of diarrhoea in people, especially children, and animals globally. Due to the segmented nature of the RVA genome, animal RVA strains have the potential to adapt to the human host through reassortment with other co‐infecting human viruses. Macaques share food and habitat with people, resulting in close interaction between these two species. This study aimed to detect and characterize RVA in rhesus macaques (Macaca mulatta) in Bangladesh. Faecal samples (N = 454) were collected from apparently healthy rhesus macaques from nine different sites in Bangladesh between February and March 2013. The samples were tested by one‐step, real‐time, reverse transcriptase‐polymerase chain reaction (PCR). Four percent of samples (n = 20; 95% CI 2.7%–6.7%) were positive for RVA. RVA positive samples were further characterized by nucleotide sequence analysis of two structural protein gene fragments, VP4 (P genotype) and VP7 (G genotype). G3, G10, P[3] and P[15] genotypes were identified and were associated as G3P[3], G3P[15] and G10P[15]. The phylogenetic relationship between macaque RVA strains from this study and previously reported human strains indicates possible transmission between humans and macaques in Bangladesh. To our knowledge, this is the first report of detection and characterization of rotaviruses in rhesus macaques in Bangladesh. These data will not only aid in identifying viral sharing between macaques, human and other animals, but will also improve the development of mitigation measures for the prevention of future rotavirus outbreaks.

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Phylogenetic analysis of partial VP7 gene of the emerging human group A rotavirus G12 strains circulating in Tunisia

Cited by 10 publications

References 53 publications

The origins of G12P[6] rotavirus strains detected in Lebanon

The origins of G12P[6] rotavirus strains detected in Lebanon

Phylogenetic analysis of VP7 and VP4 genes of the most predominant human group A rotavirus G12 identified in children with acute gastroenteritis in Himachal Pradesh, India during 2013–2016

Molecular characterization of group A rotavirus from rhesus macaques ( Macaca mulatta ) at human–wildlife interfaces in Bangladesh

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