Phylogenetic analysis of rabies virus isolated from canids in North and Northeast Brazil

Souza, Débora Nunes de; Carnieli, Pedro; Macedo, Carla Isabel; Oliveira, Rafael de Novaes; Batista, Helena Beatriz de Carvalho Ruthner; Rodrigues, A. C.; Pereira, P. M. C.; Achkar, Samira Maria; Vieira, Luiz Fernando Pereira; Kawai, Juliana Galera Castilho

doi:10.1007/s00705-016-3079-1

Cited by 14 publications

(8 citation statements)

References 28 publications

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“…14 Failure to initiate treatment or failure to follow treatment correctly can result in patients being victims of rabies. 15 Health professionals should be constantly trained and supervised as to the treatment procedures for accidents with animals potentially capable of transmitting rabies and protection of patients through the rational use of immunobiologicals, always performing a thorough epidemiological investigation of each case, as well as filling out all fields of the SINAN system notification from. 5,6,14…”

Section: Discussionmentioning

confidence: 99%

Raiva humana: avaliação da prevalência das condutas profiláticas pós-exposição no Ceará, Brasil, 2007-2015

Cavalcante

Alencar

2018

Epidemiologia e Serviços de Saúde

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Objective: to analyze the prevalence of inadequate human anti-rabies post-exposure prophylaxis in patients attacked by animals in the state of Ceará, Brazil, 2007-2015 Methods: this was a study of the prevalence of inadequate prophylaxis procedures, using data on reported cases of accidents involving animals potentially capable of transmitting rabies registered on the Notifiable Diseases Information System (SINAN) from January 2007 to December 2015. Results: of the 231,694 reported cases, 95.8% were found to have inadequate procedures, especially in young people aged under 19 (37.6%), with no difference between the sexes (p=0.04); higher prevalence rate of inadequate procedures occurred with regard to exposure to bites (PR=1.03 -95%CI 1.02;1.03), multiple injuries (PR=1.07 -95%CI 1.06;1.07), accidents involving dead/missing animals (RP=1.05 -95%CI 1.05;1.06), and vaccine treatments (RP=1.03 -95%CI 1.02;1.03). Conclusion: high prevalence of inadequate procedures indicates the need for a better epidemiological evaluation of cases and compliance with the Human Rabies Prophylaxis Technical Standards, in order to ensure adequate anti-rabies prophylaxis procedures.

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Section: Discussionmentioning

confidence: 99%

Raiva humana: avaliação da prevalência das condutas profiláticas pós-exposição no Ceará, Brasil, 2007-2015

Cavalcante

Alencar

2018

Epidemiologia e Serviços de Saúde

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“…Si bien la similitud entre estos subgrupos fue de 98,75%, la sustitución en el sitio C40G nos permitió diferenciarlos; este agrupamiento estaría relacionado con la circulación localizada (geográfica) del virus, característica del VR para mantener una relación específica con su hospedero y/o ubicación geográfica. Hallazgos similares relacionados con la filogeografía del VR también fueron observados en estudios de caracterización de la nucleoproteína del VR en cánidos del norte y noreste de Brasil, que establecen la formación de grupos como resultado de la adaptación de subpoblaciones de virus a regiones geográficas (22,23) ; así también estudios filogenéticos del VR en canes de la India y reportes de carnívoros estudiados en Brasil mencionan que el agrupamiento genético está determinado geográficamente (24,25) .…”

Section: Resultsunclassified

Caracterización molecular de la nucleoproteína del virus de la rabia en canes procedentes de Arequipa, Perú

Torpoco

Huallpa

Espinoza-Culupú

et al. 2019

Rev Peru Med Exp Salud Publica

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a Médico veterinario; b biólogo, magíster en Mejoramiento genético de plantas; c biólogo, magíster en Biología molecular, d médico veterinario, magíster en Medicina veterinaria preventiva. El artículo es parte de la tesis de Carina Mantari Torpoco para obtener el grado de magíster en Objetivos. Caracterizar la nucleoproteína (N) y establecer el origen del virus de la rabia en canes procedentes de Arequipa. Materiales y métodos. Se analizaron 30 muestras de tejido nervioso procedentes de los departamentos de Arequipa y Puno. Se extrajo el ARN total de las muestras y se sintetizó ADNc para amplificar el gen de la nucleoproteína, secuenciarlo y realizar el análisis bioinformático. Resultados. Se obtuvo la formación de un grupo definido con respecto al grupo externo (European bat lyssavirus). Este grupo fue clasificado en dos subgrupos, uno constituido por muestras procedentes de Puno y Arequipa (subgrupo A), y otro por muestras de Puno (subgrupo B), observándose una identidad nucleotídica de 99,9% en el subgrupo A. Conclusiones. Los agrupamientos de las secuencias virales muestran que los casos de rabia canina notificados en Arequipa son el resultado de la expansión de rabia canina procedente de la región endémica de Puno. ABSTRACTObjective. To characterize the nucleoprotein (N) and establish the origin of the rabies virus in dogs coming from Arequipa. Materials and Methods. Thirty samples of nervous tissue from the departments of Arequipa and Puno were analyzed. Total RNA was extracted from the samples and cDNA was synthesized to amplify the nucleoprotein gene, sequence it, and perform bioinformatics analysis. Results. A defined group was formed with respect to the external group (European bat lyssavirus). This group was classified into two subgroups, one constituted by samples coming from Puno and Arequipa (subgroup A), and another one by samples from Puno (subgroup B), exhibiting a nucleotide identity of 99.9% in subgroup A. This group was classified in two subgroups, one constituted by samples coming from Puno and Arequipa (subgroup A), and another one by samples from Puno (subgroup B), observing a nucleotide identity of 99.9% in subgroup A. Conclusions. The groupings of viral sequences show that the cases of canine rabies reported in Arequipa are the result of the expansion of canine rabies from the endemic region of Puno.

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“…Although the N protein is highly conserved including its phosphorylation site (serine at 289) and the antigenic site III (313-337), mutations have been observed at positions 374, 410, 415 and 421 for RABV/cattle/1/18 and at position 417 for RABV/donkey/7/18. These mutated amino acids corresponding to the N protein antigenic sites I and IV (374-383) and the immunodominant peptide (404-418) contribute to specific antigen and antigenic variation (Goto et al, 2000;de Souza et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…It is a highly conserved gene, so it is frequently used for genotyping lyssaviruses (Kuzmin et al, 2005). The conserved antigenic sites of the N protein have been considered a useful tool for the detection and identification of lyssaviruses in brain tissues (Goto et al, 2000;Johnson et al, 2002;Khawplod et al, 2006;Nagarajan et al, 2009;Yang et al, 2011;Orłowska and Zmudzi nski, 2014;de Souza et al, 2017;Manjunatha Reddy et al, 2018). The G protein is responsible for the viral attachment to the host cell surface and for the membrane fusion (Dietzgen et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Molecular characterisation of rabies virus detected in livestock animals in the southern part of Egypt during 2018 and 2019

Sultan

Ahmed

Abdelazeem

et al. 2021

AVet

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Brain samples were collected from 33 animals of different species, including buffalo, cattle, dog, donkey, fox and wolf, that had been suspected to be infected by rabies virus (RABV) in different geographical regions of Aswan and Luxor governorates in Egypt. The samples were submitted for histopathological examination and the presence of the nucleic acid and antigens of RABV was tested by RT-PCR and indirect fluorescent antibody technique (IFAT), respectively. Sixteen samples were found positive by all the three examinations. Three samples were selected for further study from animals in which the highest virus loads were detected. The partial sequence of the RABV N gene was determined and analysed from the samples of a buffalo, a cow and a donkey. The viruses in the samples were found to share 95–98% and 95–97% nucleotide and amino acid sequence identities, respectively. In comparison to reference sequences, a few amino acid substitutions occurred in the N protein antigenic sites I and IV in the immunodominant epitopes of the viruses detected in the cow and the donkey but not in the one from the buffalo. The phylogenetic analysis revealed that the RABVs sequenced from the samples belonged to genotype 1, Africa-4 clade, and formed two distinct sub-clades within the Egyptian clade. These findings indicate the circulation of RABV among livestock animals in the southern part of Egypt and raise public health concerns. The amino acid changes detected in this work may contribute to the antigenic diversification of RABVs.

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Phylogenetic analysis of rabies virus isolated from canids in North and Northeast Brazil

Cited by 14 publications

References 28 publications

Raiva humana: avaliação da prevalência das condutas profiláticas pós-exposição no Ceará, Brasil, 2007-2015

Raiva humana: avaliação da prevalência das condutas profiláticas pós-exposição no Ceará, Brasil, 2007-2015

Caracterización molecular de la nucleoproteína del virus de la rabia en canes procedentes de Arequipa, Perú

Molecular characterisation of rabies virus detected in livestock animals in the southern part of Egypt during 2018 and 2019

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