Phylogenetic Analysis of Reticulitermes speratus using the Mitochondrial Cytochrome C Oxidase Subunit I Gene

Cho, Moon Jung; Shin, Keum; Kim, Young Kyoon; Kim, Yeong Suk; Kim, Tae Jong

doi:10.5658/wood.2010.38.2.135

Cited by 6 publications

(6 citation statements)

References 10 publications

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“…The termites used were R. speratus KMT001 (Cho et al, 2010), which were collected in the Bukhan Mountain, Seoul, Korea (latitude: 37.614009, longitude: 126.990545). The termites were grown at 26°C and 70% humidity without light.…”

Section: Termitesmentioning

confidence: 99%

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A New Putative Chitinase from Reticulitermes speratus KMT001

Ham¹,

Park²,

Kim³

et al. 2019

Journal of the Korean Wood Science and Technology

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Termites are pests that cause serious economic and cultural damage by digesting wood cellulose. Termites are arthropods and have an epidermis surrounded by a chitin layer. To maintain a healthy epidermis, termites have chitinase (β -1,4-poly-N-acetyl glucosamidinase, EC 3.2.1.14), an enzyme that hydrolyzes the β-1,4 bond of chitin. In this study, the amino acid sequence of the gene, which is presumed to be termite chitinolytic enzyme (NCBI accession no. KC477099), was obtained from a transcriptomic analysis of Reticulitermes speratus KMT001 in Bukhan Mountain, Korea. An NCBI protein BLAST search confirmed that the protein is a glycoside hydrolase family 18 (GH18). The highest homology value found was 47%, with a chitinase from Araneus ventricosus. Phylogenetic analysis indicated that the KC477099 protein has the same origins as those of arthropods but has a very low similarity with other arthropod chitinases, resulting in separation at an early stage of evolution. The KC477099 protein contains two conserved motifs, which encode the general enzymatic characteristics of the GH18 group. The amino acid sequences Asp 156 -Trp 157 -Glu 158 , which play an important role in the enzymatic activity of the GH18 group, were also present. This study suggests that the termite KC477099 protein is a new type of chitinase, which is evolutionarily distant from other insect chitinases.

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Section: Termitesmentioning

confidence: 99%

“…GH18 has eight similar α/β-barrel structures and two conserved regions, which play an important role in enzymatic activity. All structure and regions are located on the β3 and β4 strands (Cho et al, 2010;Fukamizo, 2000;Henrissat and Bairoch, 1993;Korb et al, 2012;Kramer and Muthukrishnan, 1997;Sharma et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

A New Putative Chitinase from Reticulitermes speratus KMT001

Ham¹,

Park²,

Kim³

et al. 2019

Journal of the Korean Wood Science and Technology

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“…Lower termites, Reticulitermes speratus KMT001 (Cho et al, 2010b), were collected from Bukhan Mountain, Seoul, Korea; 50 worker termites were grown at 4°C, 10°C, 15°C, 22°C, and 26°C for 14 days, and their guts were extracted with tweezers (Kim et al, 2010). The termite gut was extracted at the same temperature as the growing conditions for the sample to maintain the bacterial flora.…”

Section: Purification Of Chromosomal Dna From Symbiotic Bacteriamentioning

confidence: 99%

“…It is quite interesting how termites can inhabit a variety of regions from tropical rainforests to frozen soil. Reticulitermes speratus is a termite species found in Korea (Cho et al, 2010b). It has been suggested that termites originally landed on the southern region of the Korean peninsula from Japan and migrated north (Park et al, 2006;Kim et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Symbiotic Bacterial Flora Changes in Response to Low Temperature in Reticulitermes speratus KMT001

Lee¹,

Kim²,

Kim³

et al. 2018

Journal of the Korean Wood Science and Technology

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Lower termites require symbiotic microbes in their gut. The microbial communities in the termites must adapt to the termite temperature. Reticulitermes speratus KMT001 from Bukhan Mountain in Seoul may require a special symbiotic microorganisms for growth in low temperature Korean habitat. A metagenomics analysis showed a dramatic change in the symbiotic bacterial flora in the gut of R. speratus KMT001 in response to low temperatures of 4°C or 10°C. Elusimicrobia, which are endosymbionts of flagellate protists, is the dominant phylum in the termite gut at ≥15°C but its population decreased drastically at low temperature. Four representative bacterial strains isolated from R. speratus KMT001 in a previous study produced maximum -glucosidase levels within the temperature range of 10°C-30°C. Elizabethkingia sp. BM10 produced -glucosidase specifically at 10°C. This strain supported the existence of symbiotic bacteria for the low temperature habitat of the termite. This identified bacterium will be a resource for studying low temperature adaptation of termites, studying the gene expression at low temperatures, and developing an industrial cellulase at low temperature.

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“…However, they also serve as ecological suppliers providing various nu-trients, including nitrogen compounds (Govorushko, 2019;Lobry de Bruyn and Conacher, 1990). They are widely distributed worldwide (Bourguignon et al, 2016), including Korea with freezing temperatures in winter (Cho et al, 2010b;Kim et al, 2012). In Korea, a representative lower termite species is Reticulitermes speratus (Cho et al, 2010b;Kim et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Characterization of Low-Temperature Enzymatic Reactions through Heterologous Expression and Functional Analysis of Two Beta-Glucosidases from the Termite Symbiotic Bacterium Elizabethkingia miricola Strain BM10

LEE,

KIM

2023

J. Korean Wood Sci. Technol.

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Lower termites need symbiotic microbes for cellulose digestion. Elizabethkingia miricola strain BM10 has been proposed as a symbiotic microbe that assists in low-temperature digestion and metabolism of Reticulitermes speratus KMT1, a termite on Bukhan Mountain, Seoul, Korea. In E. miricola strain BM10, β-glucosidase genes expressed at 10℃ were identified, and the psychrophilic enzymatic characteristic was confirmed by heterogeneously expressed proteins. Crude β-glucosidase in the culture broth of E. miricola strain BM10 showed specific enzymatic properties, and its substrate affinity was 4.69 times higher than that of Cellic CTec2. Among the genes proposed as β-glucosidase, two genes, bglB_1 and bglA_2, whose gene expression was more than doubled at 10℃ than at 30℃, were identified. They were heterogeneously expressed in Escherichia coli and identified as psychrophilic enzymes with an optimal reaction temperature of about 20℃-25℃. In this study, E. miricola strain BM10, a symbiotic bacterium of lower termites, produced psychrophilic β-glucosidases that contribute to the spread of the low-temperature habitat of a lower termite, R. speratus KMT1.

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Phylogenetic Analysis of Reticulitermes speratus using the Mitochondrial Cytochrome C Oxidase Subunit I Gene

Cited by 6 publications

References 10 publications

A New Putative Chitinase from Reticulitermes speratus KMT001

A New Putative Chitinase from Reticulitermes speratus KMT001

Symbiotic Bacterial Flora Changes in Response to Low Temperature in Reticulitermes speratus KMT001

Characterization of Low-Temperature Enzymatic Reactions through Heterologous Expression and Functional Analysis of Two Beta-Glucosidases from the Termite Symbiotic Bacterium Elizabethkingia miricola Strain BM10

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