Phylogenetic analysis of some Sporomusa sub-branch members isolated from human clinical specimens: description of Megasphaera micronuciformis sp. nov.

Marchandin, Hélène; Jumas‐Bilak, Estelle; Teyssier, Corinne; Jean-Pierre, Hélène; Buochberg, M. Simeon De; Carrière, Christian; Carlier, Jean‐Philippe

doi:10.1099/ijs.0.02378-0

Cited by 67 publications

(57 citation statements)

References 20 publications

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“…Using this new standard, phylogenetic trees, based on base differences between species, are constructed; and bacteria are classified and re-classified into new genera. Recently, the use of this technique for the identification of bacterial strains with ambiguous biochemical profiles and the discovery of novel genera and species have been reported by us and other researchers (5-7, 10, 16, 18-30).Based on the results of 16S rDNA sequencing, major changes in the taxonomy of anaerobic Gram-negative bacteria have been made in recent years (2,4,9,11,15). At the moment, about 50 genera of anaerobic Gram-negative bacteria have been described, three of which were sporulated.…”

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confidence: 99%

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Anaerospora hongkongensis Gen. Nov. Sp. Nov., a Novel Genus and Species with Ribosomal DNA Operon Heterogeneity Isolated from an Intravenous Drug Abuser with Pseudobacteremia

Woo

Teng

Leung

et al. 2005

Microbiology and Immunology

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Since the discovery of polymerase chain reaction (PCR) and DNA sequencing, comparison of the gene sequences of bacterial species has shown that the 16S rDNA is highly conserved within a species and among species of the same genus, and hence can be used as the new standard for classification of bacteria. Using this new standard, phylogenetic trees, based on base differences between species, are constructed; and bacteria are classified and re-classified into new genera. Recently, the use of this technique for the identification of bacterial strains with ambiguous biochemical profiles and the discovery of novel genera and species have been reported by us and other researchers (5-7, 10, 16, 18-30).Based on the results of 16S rDNA sequencing, major changes in the taxonomy of anaerobic Gram-negative bacteria have been made in recent years (2,4,9,11,15). At the moment, about 50 genera of anaerobic Gram-negative bacteria have been described, three of which were sporulated. In this study, we report the isolation of an anaerobic Gram-negative bacterial strain from the blood culture of an intravenous drug abuser. The strain, named HKU15 T , exhibited phenotypic characteristics that do not fit into patterns of any known genus and species. 16S rDNA sequencing showed that there was only about 90% base identity between the 16S rDNA of HKU15T and that of the most closely related species, Dendrosporobacter quercicolus (15). On the basis of these results we propose a new genus and species, Anaerospora hongkongensis gen. nov. sp. Abstract: A bacterium was isolated from the blood culture of an intravenous drug abuser with pseudobacteremia. The cells were strictly anaerobic, straight or slightly curved, sporulating, Gram-negative rods. It grew on sheep blood agar as non-hemolytic, pinpoint colonies after 48 hr of incubation at 37 C in an anaerobic environment. It was motile but did not produce catalase or cytochrome oxidase. 16S ribosomal DNA (rDNA) sequencing revealed three different copies of 16S rDNA sequences. More than 90% of the differences among them were due to differences in the lengths of the sequences. Phylogenetically, the bacterium is clustered with Dendrosporobacter, Sporomusa, and Propionispora, the other three genera of anaerobic, sporulating, Gram-negative rods. There were 8.6-11.1% differences between the 16S rDNA sequences of the bacterium and that of D. quercicolus, 4.7-15.1% differences between the 16S rDNA sequences of it and those of S. acidovorans, S. aerivorans, S. malonica, S. ovata, S. paucivorans, S. silvacetica, S. spaeroides, and S. termitida, and 7.6-13.1% differences between the 16S rDNA sequences of it and those of P. hippei and P. vibrioides. The G؉C content of the bacterium (mean؎SD) was 46.8؎3.2%. For these reasons, a new genus and species, Anaerospora hongkongensis gen. nov. sp. nov., is proposed, for which HKU15 T is the type strain. Anaerospora hongkongensis

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mentioning

confidence: 99%

“…Based on the results of 16S rDNA sequencing, major changes in the taxonomy of anaerobic Gram-negative bacteria have been made in recent years (2,4,9,11,15). At the moment, about 50 genera of anaerobic Gram-negative bacteria have been described, three of which were sporulated.…”

mentioning

confidence: 99%

Anaerospora hongkongensis Gen. Nov. Sp. Nov., a Novel Genus and Species with Ribosomal DNA Operon Heterogeneity Isolated from an Intravenous Drug Abuser with Pseudobacteremia

Woo

Teng

Leung

et al. 2005

Microbiology and Immunology

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“…Previously, Gram negative anaerobic bacteria belonging to the genus Pectinatus were affiliated to sub branch sporomusa in the family Acidamincocaceae of class Clostridia [82]- [84]. However in 2010, a new class Negativicutes bacteria having a Gram negative cell wall, was proposed within the phylum Fermicutes along with a new order, Selenomonadales [85] which has changed the taxonomic status of the genus Pectinatus affiliating it to class-Negativicutes [85], Order-Selenomonadales [85] [80].…”

Section: Pectinatusmentioning

confidence: 99%

“…Megasphaera species have been isolated from a variety of different environments such as human clinical specimens, rumen gut flora and brewery environments [84] [100]. Important characteristics of beer spoilage Megasphaera species are shown in Table 2.…”

Section: Megasphaeramentioning

confidence: 99%

“…Genus Megasphaera, originally described by Rogosa [98], consists of five validly published species; M. elsdenii [98], M. cerevisiae [99], M. micronuciformis [84], M. paucivorans and M. sueciensis [77]. Megasphaera species have been isolated from a variety of different environments such as human clinical specimens, rumen gut flora and brewery environments [84] [100].…”

Section: Megasphaeramentioning

confidence: 99%

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Review: Gram Negative Bacteria in Brewing

Ashtavinayak¹,

Elizabeth²

2016

AiM

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Gram negative aerobic bacteria such as Acetic Acid Bacteria, which include Acetobacter and Gluconobacter, have historically caused significant problems to brewers. Although incidences of spoilage have recently reduced as a result of improvements in beer packaging, these bacteria are still a concern in dispense systems in pub breweries, public houses and cask conditioned beers. Gram negative facultative bacteria of the genus Zymomonas can spoil primed cask conditioned beer and cider. There is a wide range of Enterobacteraeceace which are found within brewery environments and they serve as indicator microorganisms for hygiene and sanitation. Gram negative strictly anaerobic bacteria such as Pectinatus and Megasphaera have recently emerged as a significant threat due to the improvement in reduction of oxygen levels in beer and an increase in production of unpasteurised beer. Pectinatus and Megasphaera are sensitive to routine cleaning agents used in breweries, but they can survive and proliferate in biofilms eventually causing spoilage of beer. This review focuses on Gram negative aerobic, facultative anaerobic and strictly anaerobic brewery related spoilage bacteria.

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Veillonellaand Other Anaerobic Gram-Negative Cocci

Brazier

2010

Topley &Amp; Wilson's Microbiology and Microbial Infections

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Phylogenetic analysis of some Sporomusa sub-branch members isolated from human clinical specimens: description of Megasphaera micronuciformis sp. nov.

Cited by 67 publications

References 20 publications

Anaerospora hongkongensis Gen. Nov. Sp. Nov., a Novel Genus and Species with Ribosomal DNA Operon Heterogeneity Isolated from an Intravenous Drug Abuser with Pseudobacteremia

Anaerospora hongkongensis Gen. Nov. Sp. Nov., a Novel Genus and Species with Ribosomal DNA Operon Heterogeneity Isolated from an Intravenous Drug Abuser with Pseudobacteremia

Review: Gram Negative Bacteria in Brewing

Veillonellaand Other Anaerobic Gram-Negative Cocci

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