Phylogenetic analysis of the genus Actinoplanes and transfer of Actinoplanes minutisporangius Ruan et al. 1986 and 'Actinoplanes aurantiacus' to Cryptosporangium minutisporangium comb. nov. and Cryptosporangium aurantiacum sp. nov.

Tamura, Tomohiko; Hatano, Kazunori

doi:10.1099/00207713-51-6-2119

Cited by 133 publications

(84 citation statements)

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“…As various actinomycetes belonging to the genera Actinoplanes, Kineosporia [22][23][24] and Cryptosporangium 15,25 were frequently isolated from leaf-litter samples, as evidenced also by other reports, 26,27 it is conceivable that they may have an important role in the degradation of fallen leaves. It should be noted that these three genera belong to different families (Micromonosporaceae, Kineosporiaceae and Cryptosporangiaceae, respectively), which in turn belong to different suborders (Micromonosporineae, Kineosporiineae and Frankineae, respectively).…”

Section: Genus-level Diversitysupporting

confidence: 52%

“…The 16S ribosomal RNA (rRNA) gene was amplified by PCR using TaKaRa Ex Taq (Takara Bio, Otsu City, Shiga, Japan) with the primers, 9F (5¢-GAGTTTGATCCTGGCTCAG-3¢) and 1541R (5¢-AAGGAGGTGATCCAGCC-3¢), or occasionally 1510R (5¢-GGC TACCTTGTTACGA-3¢). Almost the entire sequence of the 16S rRNA gene (1300-1400 bp) was amplified by PCR as reported by Tamura and Hatano 15 and directly sequenced using an ABI Prism BigDye Terminator cycle sequencing kit (Applied Biosystems, Foster City, CA, USA) and an ABI Model 3730 automatic DNA sequencer. The 16S rRNA gene sequence was compared with other sequences in the EMBL/GenBank/DDBJ database using BLAST searches and in the EzTaxon 16 database, which includes only type strain sequences.…”

Section: S Rrna Gene Sequencing and Phylogenetic Analysismentioning

confidence: 99%

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Taxonomic and ecological studies of actinomycetes from Vietnam: isolation and genus-level diversity

et al. 2011

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Actinomycetes were isolated from 109 soil and 93 leaf-litter samples collected at five sites in Vietnam between 2005 and 2008 using the rehydration-centrifugation (RC) method, sodium dodecyl sulfate-yeast extract dilution method, dry-heating method and oil-separation method in conjunction with humic acid-vitamin agar as an isolation medium. A total of 1882 strains were identified as Vietnamese (VN)-actinomycetes including 1080 (57%) streptomycetes (the genus Streptomyces isolates) and 802 (43%) non-streptomycetes. The 16S ribosomal RNA gene sequences of the VN-actinomycetes were analyzed using BLAST searches. The results showed that these isolates belonged to 53 genera distributed among 21 families. Approximately 90% of these strains were members of three families: Streptomycetaceae (1087 strains, 58%); Micromonosporaceae (516 strains, 27%); and Streptosporangiaceae (89 strains, 5%). Motile actinomycetes of the genera Actinoplanes, Kineosporia and Cryptosporangium, which have quite common morphological characteristics, were frequently isolated from leaf-litter samples using the RC method. It is possible that these three genera acquired common properties during a process of convergent evolution. By contrast, strains belonging to the suborder Streptosporangineae were exclusively isolated from soils. A comparison of the sampling sites revealed no significant difference in taxonomic diversity between these sites. Among the non-streptomycetes, 156 strains (19%) were considered as new taxa distributed into 21 genera belonging to 12 families. Interestingly, the isolation of actinomycetes from leaf-litter samples using the RC method proved to be the most efficient way to isolate new actinomycetes in Vietnam, especially the Micromonosporaceae species.

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Section: Genus-level Diversitysupporting

confidence: 52%

Section: S Rrna Gene Sequencing and Phylogenetic Analysismentioning

confidence: 99%

Taxonomic and ecological studies of actinomycetes from Vietnam: isolation and genus-level diversity

et al. 2011

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“…23 PCR product was directly sequenced using a BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA) and an automatic DNA sequencer (ABI Prism 3730 Genetic Analyzer; Applied Biosystems).…”

Section: Phylogenetic Analysismentioning

confidence: 99%

Flexivirga alba gen. nov., sp. nov., an actinobacterial taxon in the family Dermacoccaceae

et al. 2011

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“…DNA was extracted as described by Marmur (1961) and Saito & Miura (1963) but with a slight modification: after lysis, 20 % SDS (50 ml) and proteinase K (50 ml, 4 mg ml 21 , Nacalai Tesque) were used to denature and degrade proteins and phenol/chloroform/isoamyl alcohol (25 : 24 : 1, v/v) was used to remove denatured proteins. The 16S rRNA gene sequences were analysed as described by Tamura & Hatano (2001), using an ABI Prism BigDye Terminator cycle sequencing kit (Applied Biosystems) and an automatic DNA sequencer (ABI Prism 3130 Genetic Analyzer; Applied Biosystems). The CLUSTAL_X program (Thompson et al, 1997) was used to align the 16S rRNA gene sequence with corresponding sequences from public databases for all of the type strains in the family Dermacoccaceae.…”

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confidence: 99%

Luteipulveratus mongoliensis gen. nov., sp. nov., an actinobacterial taxon in the family Dermacoccaceae

Ara

Yamamura

Tsetseg

et al. 2010

International Journal of Systematic and Evolutionary Microbiology

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Luteipulveratus mongoliensis gen. nov., sp. nov., an actinobacterial taxon in the family Dermacoccaceae A novel actinobacterial strain, MN07-A0370 T , was isolated from Mongolian soil and its taxonomic status was determined using a polyphasic approach. Comparative 16S rRNA gene sequence studies revealed that strain MN07-A0370 T represented a novel lineage within the actinobacteria. Strain MN07-A0370 T formed a distinct clade in the family Dermacoccaceae and was most closely related to the members of the genera Dermacoccus (16S rRNA gene sequence similarity, 96.2 %-96.4 %), Demetria (94.1 %) and Kytococcus (93.7 %). The cell-wall peptidoglycan of the novel strain contained L-lysine, alanine, aspartic acid, glutamic acid and serine and represented peptidoglycan type A4a. The menaquinones were MK-8(H 4 ) and MK-8(H 6 ). The polar lipids were phosphatidylglycerol, diphosphatidylglycerol and phosphatidylinositol and the whole-cell sugars were galactose, mannose, rhamnose, ribose and glucose. Mycolic acids were absent. The fatty acid profile was characterized by the presence of large amounts of saturated iso-and anteisobranched-chain fatty acids as well as smaller amounts of saturated straight-chain and unsaturated acids. The major fatty acids were iso-C 16 : 0 , anteiso-C 17 : 0 , iso-C 16 : 1 H, C 17 : 1 v9c and C 17 : 0 10-methyl. The G+C content of the DNA was 68.2 mol%. On the basis of chemotaxonomic, physiological and biochemical differences from other genera of the family Dermacoccaceae, strain MN07-A0370 T should be classified as representing a novel species in a new genus, for which we propose the name Luteipulveratus mongoliensis gen. nov., sp. nov. The type strain is MN07-A0370 T (5NBRC 105296 T 5VTCC D9-09 T ).Mongolia is one of the largest Asian countries and is unique in its environmental traits. Recently, these environments have attracted the attention of naturalists and other researchers looking for novel actinomycetes (Norovsuren et al., 2007). In a programme aimed at isolating novel rare actinomycetes from Mongolia as a potential source of bioactive secondary metabolites, strain MN07-A0370 T , in the family Dermacoccaceae (Stackebrandt & Schumann, 2000), was studied to establish its taxonomic status. The family Dermacoccaceae currently contains three recognized genera, Dermacoccus and Kytococcus (Stackebrandt et al., 1995) and Demetria (Groth et al., 1997a). Isolates assigned to this family are typically associated with terrestrial habitats, notably cured meat products, skin, human blood and soil (De la Rosa et al., 1990;Cordero & Zumalacarregui, 2000;Becker et al., 2002; Papamanoli et al., 2002).Samples of grassland soil were collected in July 2007 from Terelj National Park, Töv Province, Ulaanbaatar, Mongolia. The samples were dried at room temperature for 5-7 days, rehydrated and centrifuged (Hayakawa et al., 2000) and used to inoculate humic acid-vitamin agar (Hayakawa & Nonomura, 1987) containing (l 21 ) 20 mg trimethoprim and 10 mg nalidixic acid. Strain MN07-A0370 T was isolated after incubat...

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Phylogenetic analysis of the genus Actinoplanes and transfer of Actinoplanes minutisporangius Ruan et al. 1986 and 'Actinoplanes aurantiacus' to Cryptosporangium minutisporangium comb. nov. and Cryptosporangium aurantiacum sp. nov.

Cited by 133 publications

References 22 publications

Taxonomic and ecological studies of actinomycetes from Vietnam: isolation and genus-level diversity

Taxonomic and ecological studies of actinomycetes from Vietnam: isolation and genus-level diversity

Flexivirga alba gen. nov., sp. nov., an actinobacterial taxon in the family Dermacoccaceae

Luteipulveratus mongoliensis gen. nov., sp. nov., an actinobacterial taxon in the family Dermacoccaceae

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