Phylogenetic analysis of the genus Borrelia: a comparison of North American and European isolates of Borrelia burgdorferi

Marconi, Richard T.; Garon, Claude F.

doi:10.1128/jb.174.1.241-244.1992

Cited by 89 publications

(87 citation statements)

References 24 publications

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“…The 16S rDNA sequences of two distantly related species of spirochaetes, Borrelia burgdorferi B31 T (accession no. X57404) (Marconi & Garon, 1992) The outgroup for the nox sequences was designated Enterococcus faecalis 10C1 (accession no. X68847) (Ross & Claiborne, 1992).…”

Section: Methodsmentioning

confidence: 99%

Analysis of genetic variation in Brachyspira aalborgi and related spirochaetes determined by partial sequencing of the 16S rRNA and NADH oxidase genes

Mikosza

Munshi

Hampson

2004

Journal of Medical Microbiology

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The purpose of this study was to investigate genetic variation in the anaerobic intestinal spirochaete Brachyspira aalborgi by partial sequencing of the 16S rRNA and NADH oxidase genes. The spirochaete is poorly cultivable; hence, only six isolates were available for analysis. Additional sequences were amplified from DNA extracted from fixed colorectal biopsies from 26 patients with histological evidence of intestinal spirochaetosis, and from the faeces of six non-human primates (NHP). Multiple biopsies from sites along the large intestine were tested from three of the 26 patients. Sequences from two biopsies were closely related to those of the spirochaete Brachyspira pilosicoli. Eight B. aalborgi-like 16S rDNA sequences were generated from the biopsies from the other 24 patients, and four from the NHP faeces. The B. aalborgi 16S rDNA sequences were divided into three clusters, 1, 2 and 4, with individual sequence similarities to the type strain ranging from 97 . 49 to 100 %. All human isolates of B. aalborgi were located in cluster 1, as was the sequence of the so-called 'Brachyspira ibaraki'. All four 16S rDNA sequences from the NHP faeces and the two NHP isolates of B. aalborgi were located in cluster 4, which was distinct. Cluster 4 may represent a novel Brachyspira species. Evidence for multiple strains of B. aalborgi or other Brachyspira species was found in biopsies from two patients. In the three individuals from whom multiple biopsies were amplified, the sequences at each intestinal site were the same, indicating the presence of one dominant strain.

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Section: Methodsmentioning

confidence: 99%

Analysis of genetic variation in Brachyspira aalborgi and related spirochaetes determined by partial sequencing of the 16S rRNA and NADH oxidase genes

Mikosza

Munshi

Hampson

2004

Journal of Medical Microbiology

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“…There are a number of possibilities for the observed sequence differences. There is evidence of very little diversity of the 16s rDNA sequences of Francisella [26] and Legionella pneumophila [27], whereas for Borrelia burgdorferi strains, up to 1.0% divergence has been demonstrated [28]. Eckloff et al [29] compared the 16s rDNA sequences from five isolates of Helicobacter pylori and found deviations from the consensus sequence at 13 separate positions which could be attributed to clonal origins.…”

Section: Discussionmentioning

confidence: 99%

Phylogenetic analysis of Calymmatobacterium granulomatis based on 16S rRNA gene sequences

Kharsany¹,

Hoosen²,

Kiepiela³

et al. 1999

Journal of Medical Microbiology

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Calymmatobacterium granulomatis is the aetiological agent of granuloma inguinale -a chronic granulomatous genital infection -and is morphologically similar to members of the genus Klebsiella. This study determined the 16s rRNA gene sequence of C. granulomatis and the taxonomic position of the organism in relation to the genus Klebsiella. Genomic DNA was extracted from C. granulomatis-infected monocytes and from frozen and formalin-fixed paraffin wax-embedded tissue biopsy specimens from patients with histologically proven granuloma inguinale. The 16s rDNA was amplified by PCR with broad range oligonucleotide primers. The amplified DNA fragments were cloned into PMOS vector, digested with Bum HI and Pstl restriction endonucleases, hybridised with a gram-negative bacterial probe (DL04), sequenced in both directions by the automated ALFTM DNA sequencer, verified on an ABI Prism 377 automated sequencer and analysed with DNASIS and MEGA software packages. Sequence analysis revealed DNA homology of 99% in C. granulomatis from the different sources, supporting the belief that the bacteria in the culture and the biopsy specimens belonged to the same species, although there was some diversity within the species. Phylogenetically, the strains were closely related to the genera Klebsiella and Enterobacter with similarities of 95% and 94% respectively. C. granulomatis is a unique species, distinct from other related organisms belonging to the y subclass of Proteobacteria.

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“…Phylogenetic analysis was performed as described previously by Marconi et al (20). Briefly, the 22 16S rRNA or rDNA sequences were aligned using the Clustal V computer program (11) and 1,126-base masked sequences were generated by removing gaps and unidentified nucleotide positions.…”

Section: Methodsmentioning

confidence: 99%

“…Sequence analysis of the cloned 16S rRNA genes revealed 1,536 bases of nearly complete sequences including both primer regions (Head and End). For the phylogenetic analysis, 16S rRNA gene sequences of five Korean isolates were aligned with other published sequences (20)(21)(22)32) and 1,126-base masked sequences were generated. Using these masked sequences, the percent sequence similarity values (Table 2) and evolutionary distance were calculated.…”

Section: Methodsmentioning

confidence: 99%

Genetic Analysis of Borrelia burgdorferi Sensu Lato in Korea Using Genomic Hybridization and 16S rRNA Gene Sequence Determination

Kee

Yoon²,

et al. 1996

Microbiology and Immunology

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Nine Borrelia burgdorferi sensu lato isolated in Korea were subjected to genomic hybridization using 16S rRNA gene probe and specific restriction patterns (HindIII and EcoRV) led these nine Borrelia into five subtypes. The evolutionary relationships of the five isolates corresponding to five RFLP groups were measured through the sequence determination of 16S rRNA gene and phylogenetic analysis. The isolates 935T (group I), 934U and 17Y (Group IIa, IIb) were well clustered with B. garinii and B. afzelii. 5MT and 9MT strains (Group IIIa and Group IIIb) formed a common branch shared with B. afzelii cluster although the evolutionary distance was rather long. So, most of B. burgdorferi sensu lato in Korea was B. afzelii or B. afzelii-related group and some minor group such as B. garinii also existed.

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Phylogenetic analysis of the genus Borrelia: a comparison of North American and European isolates of Borrelia burgdorferi

Cited by 89 publications

References 24 publications

Analysis of genetic variation in Brachyspira aalborgi and related spirochaetes determined by partial sequencing of the 16S rRNA and NADH oxidase genes

Analysis of genetic variation in Brachyspira aalborgi and related spirochaetes determined by partial sequencing of the 16S rRNA and NADH oxidase genes

Phylogenetic analysis of Calymmatobacterium granulomatis based on 16S rRNA gene sequences

Genetic Analysis of Borrelia burgdorferi Sensu Lato in Korea Using Genomic Hybridization and 16S rRNA Gene Sequence Determination

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