Phylogenetic Analysis of theArenaviridae:Patterns of Virus Evolution and Evidence for Cospeciation between Arenaviruses and Their Rodent Hosts

Bowen, Michael D.; Peters, C. J.; Nichol, Stuart T.

doi:10.1006/mpev.1997.0436

Cited by 209 publications

(187 citation statements)

References 47 publications

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“…The results of a recent analysis of genetic data suggest that the diversity of the arenaviruses is the product of long-term coevolution with their principal hosts. 9 Presently, the WWA virus is known only from 2 strains recovered from N. albigula collected from Whitewater Arroyo in northwestern New Mexico. 3 Assuming that the association between WWA virus and N. albigula represents a long-term, shared evolutionary relationship, then Neotoma species in California may be infected with viruses phylogenetically closely related to WWA virus.…”

Section: Discussionmentioning

confidence: 99%

Arenavirus antibody in rodents indigenous to coastal southern California.

Bennett

Milazzo²,

Webb³

et al. 2000

The American Journal of Tropical Medicine and Hygiene

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Section: Discussionmentioning

confidence: 99%

Arenavirus antibody in rodents indigenous to coastal southern California.

Bennett

Milazzo²,

Webb³

et al. 2000

The American Journal of Tropical Medicine and Hygiene

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“…Reverse transcription and polymerase chain amplification of a partial length region of the arenavirus nucleocapsid protein gene was carried out by using the Access RT-PCR Kit (Promega Corp., Madison, WI) in conjunction with oligonucleotides 1010C and NW1696R. 8 These oligonucleotides prime the reverse transcription and polymerase chain amplification of a 616-nt region near the 3Ј end of the WWA virus nucleocapsid protein gene. 5 Size separation of the PCR products was done by electrophoresis; and the PCR product of the expected size was purified from an agarose gel slice, using the QIAquick Gel Extraction Kit (Qiagen, Inc.).…”

Section: Methodsmentioning

confidence: 99%

Experimental infection of Neotoma albigula (Muridae) with Whitewater Arroyo virus (Arenaviridae).

Fulhorst

Milazzo²,

Bradley³

et al. 2001

The American Journal of Tropical Medicine and Hygiene

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Abstract. The Whitewater Arroyo virus (WWA) is a newly described North American arenavirus. The purpose of this study was to elucidate the biology of this virus in its natural rodent host, Neotoma albigula (white-throated woodrat). Thirteen adult, 7 juvenile, and 8 newborn woodrats each were inoculated subcutaneously with 1,000 cell culture infectious dose 50 of the WWA virus prototype strain AV 9310135. All 28 animals became infected (as measured by the recovery of infectious virus and/or seroconversion) and no overt illness was associated with infection. Infection and virus shedding in the adult animals were transient (less than 59 days) whereas virus shedding in animals inoculated at birth persisted through 164 days of age. These results indicate that the duration of WWA virus infection in N. albigula is dependent upon the animal's age at the onset of infection and that neonatal infection can result in chronic (perhaps lifelong) virus shedding.

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“…A selection of samples found negative by the GPC PCR were subsequently retested by PCR targeted at a fragment of the nucleoprotein (N) gene (8), by using primers to sequences common to the Old World arenaviruses. Total RNA was extracted by using QIAamp viral RNA mini-kit (QIAGEN, Crawley, UK), converted to cDNA, and amplifi ed by using a single-step kit (Superscript III one-step RT-PCR with Platinum Taq polymerase system; Invitrogen, Paisley, UK) in conjunction with oligonucleotides arena1 + and LCMV322 -(10) or 1010C and either OW1696R or NW1696R (8). Products were separated and visualized by agarose gel electrophoresis, and amplicons were purifi ed with the QIAquick PCR purifi cation kit (QIAGEN).…”

Section: The Studymentioning

confidence: 99%