Phylogenetic analysis ofIridaceae with parsimony and distance methods using the plastid generps4

Souza-Chies, Tatiana T.; Bittar, Gabriel; Nadot, Sophie; Carter, Leigh; Besin, Evelyne; Lejeune, Bernard

doi:10.1007/bf00982535

Cited by 177 publications

(62 citation statements)

References 31 publications

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“…The three most variable ones, ITS, trnG, and rps4 were selected for the investigation. ITS was amplified with the primers '18SF' and '26SR' (Rydin et al 2004), in a few cases with the internal primers '5.8F-Chrys' (Howis et al 2009) and '5.8SRPEny' (Nylinder et al 2013), trnG with the primers 'trnGf' and 'trnGr' (Pacak & Szweykowska-Kulińska 2000), and rps4 with 'rps5F' (Nadot et al 1994) and 'trnS' (Souza- Chies et al 1997). For all three markers the following PCR program was used: 5 min at 95°C followed by 4 cycles of 30 sec at 95°C, 30 sec at 57°C, 1 min at 72°C, 4 cycles of 30 sec at 95°C, 30 sec at 55°C, 1 min at 72°C and 34 cycles of 30 sec at 95°C, 30 sec at 57°C, 1 min at 72°C and a final elongation step of 8 min at 72°C.…”

Section: Molecular Methodsmentioning

confidence: 99%

Scandinavian Oncophorus (Bryopsida, Oncophoraceae): species, cryptic species, and intraspecific variation

Hedenäs

2017

EJT

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Abstract. Scandinavian members of the acrocarpous moss genus Oncophorus were revised after field observations had suggested unrecognized diversity. Based on molecular (nuclear: internal transcribed spacers 1 and 2, ITS; plastid: trnG UCC G2 intron, trnG, rps4 gene + trnS-rps4 spacer, rps4) and morphological evidence, four morphologically distinguishable species are recognized, Oncophorus elongatus (I.

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Section: Molecular Methodsmentioning

confidence: 99%

Scandinavian Oncophorus (Bryopsida, Oncophoraceae): species, cryptic species, and intraspecific variation

Hedenäs

2017

EJT

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“…Markers were amplified in 25 μL total volume including 50-150 ng genomic DNA, 1 × PCR buffer, 200 μM of each dNTP, 1 μM of each primer, and 0.65 U Ex Taq polymerase (TaKaRa Bio, Shiga, Japan). Previously published primers were used for trnL-F (Taberlet et al 1991; primers e and f), rbcL (Little and Barrington 2003), and rps4-trnS (Souza-Chies et al 1997;Li et al 2008). The PCR products were purified with ExoSAP-IT (USB Corp., Cleveland, Ohio) and sequenced in both directions using an ABI Prism 3130x1 automated sequencer (DNA Analysis Facility, Vermont Cancer Center, Burlington, Vermont) with the ABI Prism BigDye Terminator cycle sequence ready reaction kit (Applied Biosystems, Foster City, California).…”

Section: Methodsmentioning

confidence: 99%

The Utility of Nuclear <I>gapCp</I> in Resolving Polyploid Fern Origins

et al. 2008

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“…Four chloroplast regions, including rpoC1 (Chase et al, 2007), rps4 (Nadot et al, 1994;Souza-Chies et al, 1997), trnG (Pacak and Szweykowska-Kulinska, 2000), and trnL-trnF (Taberlet et al, 1991) were selected for exhibiting the appropriate level of polymorphism at the species level in Bryoxiphium. These loci were amplified and sequenced following the protocols of Patiño et al (2015a).…”

Section: Taxon and Molecular Samplingmentioning

confidence: 99%

Is the sword moss (Bryoxiphium) a preglacial Tertiary relict?

Patiño

Goffinet

Sim‐Sim

et al. 2016

Molecular Phylogenetics and Evolution

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a b s t r a c tThe disjunction of floras between East Asia, Southeast North America, West North America, and Southwest Eurasia has been interpreted in terms of the fragmentation of a once continuous mixed mesophytic forest that occurred throughout the Northern Hemisphere due to the climatic and geological changes during the late Tertiary. The sword moss, Bryoxiphium, exhibits a distribution that strikingly resembles that of the mesophytic forest elements such as Liriodendron and is considered as the only living member of an early Tertiary flora in Iceland. These hypotheses are tested here using molecular dating analyses and ancestral area estimations. The results suggest that the extant range of Bryoxiphium results from the fragmentation of a formerly wider range encompassing North America and Southeast Asia about 10 million years ago. The split of continental ancestral populations is too recent to match with a continental drift scenario but is spatially and temporally remarkably congruent with that observed in Tertiary angiosperm relict species. The timing of the colonization of Iceland from Macaronesian ancestors, about two million years ago, is, however, incompatible with the hypothesis that Bryoxiphium is the only living member of an early Tertiary flora of the island. Alaska was recurrently colonized from East Asia. The ability of Bryoxiphium to overcome large oceanic barriers is further evidenced by its occurrence on remote oceanic archipelagos. In particular, Madeira was colonized twice independently from American and East Asian ancestors, respectively. The striking range disjunction of Bryoxiphium is interpreted in terms of its mating system, as the taxon exhibits a very singular pattern of spatial segregation of the sexes.

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Phylogenetic analysis ofIridaceae with parsimony and distance methods using the plastid generps4

Cited by 177 publications

References 31 publications

Scandinavian Oncophorus (Bryopsida, Oncophoraceae): species, cryptic species, and intraspecific variation

Scandinavian Oncophorus (Bryopsida, Oncophoraceae): species, cryptic species, and intraspecific variation

The Utility of Nuclear <I>gapCp</I> in Resolving Polyploid Fern Origins

Is the sword moss (Bryoxiphium) a preglacial Tertiary relict?

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