Phylogenetic analysis revealed that Salmonella Typhimurium ST313 isolated from humans and food in Brazil presented a high genomic similarity

Seribelli, Amanda Aparecida; Gonzales, Júlia Cunha; Almeida, Fernanda Martins de; Benevides, Leandro de Jesus; Medeiros, Marta Inês Cazentini; Rodrigues, Dália dos Prazeres; Soares, Siomar de Castro; Allard, Marc W.; Falcão, Juliana Pfrimer

doi:10.1007/s42770-019-00155-6

Cited by 8 publications

(9 citation statements)

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“…However, ST313 has caused a significant mortality rate in sub-Saharan Africa and has been described in recent years in Brazil [39]. In the present study, all the ST313 isolates presented the plasmid pSLT carrying the spvABCDR operon, pefABCD operon, rck and mig-5 genes showing a high genomic similarity among each other regardless of the isolation source as previously described [40].…”

Section: Plos Onesupporting

confidence: 77%

Phenotypic and genotypic characterization of Salmonella Typhimurium isolates from humans and foods in Brazil

et al. 2020

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Salmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium) causes gastroenteritis in many countries. However, in Brazil there are few studies that have conducted a virulence characterization of this serovar. The aim of this study was to evaluate the virulence potential of S. Typhimurium strains isolated in Brazil. Forty S. Typhimurium strains isolated from humans (n = 20) and food (n = 20) from Brazil were studied regarding their invasion and survival in human epithelial cells (Caco-2) and macrophages (U937). Their virulence potential was determined using the Galleria mellonella larvae model combined with the analysis of virulence genes by whole genome sequencing (WGS). A total of 67.5% of the S. Typhimurium studied (32.5% isolated from humans and 35% isolated from food) invaded Caco-2 epithelial cells at levels similar to or greater than the S. Typhimurium SL1344 prototype strain. In addition, 37.5% of the studied strains (25% isolated from humans and 12.5% isolated from food) survived in U937 human macrophages at levels similar to or greater than SL1344. S. Typhimurium strains isolated from humans (40%) and food (25%) showed high or intermediate virulence in G. mellonella larvae after seven days exposure. Approximately, 153 virulence genes of chromosomal and plasmidial origin were detected in the strains studied. In conclusion, the ability of the S. Typhimurium to invade Caco-2 epithelial cells was strain dependent and was not related to the source or the year of isolation. However, S. Typhimurium strains isolated from humans showed greater survival rates in U937 human macrophages, and presented higher proportion of isolates with a virulent profile in G. mellonella in comparison to strains isolated from food suggesting that this difference may be related to the higher frequency of human isolates which contained plasmid genes, such as spvABCDR operon, pefABCD operon, rck and mig-5.

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Section: Plos Onesupporting

confidence: 77%

Phenotypic and genotypic characterization of Salmonella Typhimurium isolates from humans and foods in Brazil

et al. 2020

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“…We reported that 94.4% of S. Typhimurium isolates in Peru belong to sequence type ST19. This was expected because ST19 is among the most predominant ST associated with gastroenteritis cases worldwide, and it is the most ancestral and diverse phylogenetically ST for the serovar Typhimurium [ 11 , 19 , 20 ].…”

Section: Discussionmentioning

confidence: 99%

“…It has already been shown that subclades are under different anthropogenic selection pressures. Antibiotic use might provide the selection pressure driving the emergence of sub-lineages containing AMR genes [ 10 , 11 , 19 , 66 ]. MDR strains harbour variable numbers of resistant plasmids reported mainly from livestock samples and outbreaks in hospitals and foods [ 19 , 20 ].…”

Section: Discussionmentioning

confidence: 99%

“…For example, the MDR DT104 group disseminated rapidly globally [ 9 ], and ST313, an MDR group, is responsible for invasive diseases in Africa [ 10 ]. Apart from chromosomal mobile AMR genes mobile genetic elements, such as plasmids and pathogenic islands, are essential in expanding AMR distribution among the population and are often associated with hospital-acquired infections and foodborne outbreaks [ 8 , 9 , 10 , 11 , 12 ]. MDR Salmonella isolated in various countries largely contain genes for β-lactam, tetracycline, aminoglycoside, and quinolone resistance on plasmids [ 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

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WGS-Based Lineage and Antimicrobial Resistance Pattern of Salmonella Typhimurium Isolated during 2000–2017 in Peru

et al. 2022

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Salmonella Typhimurium is associated with foodborne diseases worldwide, including in Peru, and its emerging antibiotic resistance (AMR) is now a global public health problem. Therefore, country-specific monitoring of the AMR emergence is vital to control this pathogen, and in these aspects, whole genome sequence (WGS)—based approaches are better than gene-based analyses. Here, we performed the antimicrobial susceptibility test for ten widely used antibiotics and WGS-based various analyses of 90 S. Typhimurium isolates (human, animal, and environment) from 14 cities of Peru isolated from 2000 to 2017 to understand the lineage and antimicrobial resistance pattern of this pathogen in Peru. Our results suggest that the Peruvian isolates are of Typhimurium serovar and predominantly belong to sequence type ST19. Genomic diversity analyses indicate an open pan-genome, and at least ten lineages are circulating in Peru. A total of 48.8% and 31.0% of isolates are phenotypically and genotypically resistant to at least one antibiotic, while 12.0% are multi-drug resistant (MDR). Genotype–phenotype correlations for ten tested drugs show >80% accuracy, and >90% specificity. Sensitivity above 90% was only achieved for ciprofloxacin and ceftazidime. Two lineages exhibit the majority of the MDR isolates. A total of 63 different AMR genes are detected, of which 30 are found in 17 different plasmids. Transmissible plasmids such as lncI-gamma/k, IncI1-I(Alpha), Col(pHAD28), IncFIB, IncHI2, and lncI2 that carry AMR genes associated with third-generation antibiotics are also identified. Finally, three new non-synonymous single nucleotide variations (SNVs) for nalidixic acid and eight new SNVs for nitrofurantoin resistance are predicted using genome-wide association studies, comparative genomics, and functional annotation. Our analysis provides for the first time the WGS-based details of the circulating S. Typhimurium lineages and their antimicrobial resistance pattern in Peru.

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“…In recent years in Africa, the invasive Sequence Type 313 (ST313) of S. Typhimurium has been more prevalent than ST19 (Mandomando et al, 2015), the commonly zoonotic worldwide agent of gastroenteritis. ST313 has also expanded worldwide and is now found in the United Kingdom (Ashton et al, 2017) and Brazil (Seribelli et al, 2020). Although the genome of ST313 and ST19 are 95% identical (Canals et al, 2019), ST313 had undergone genome degradation, as is observed in the hostadapted S. Typhi.…”

Section: Invasive Non-typhoidal Salmonellosis (Ints)mentioning

confidence: 99%

New Insights on the Early Interaction Between Typhoid and Non-typhoid Salmonella Serovars and the Host Cells

et al. 2021

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Salmonella enterica is a common source of food and water-borne infections, causing a wide range of clinical ailments in both human and animal hosts. Immunity to Salmonella involves an interplay between different immune responses, which are rapidly initiated to control bacterial burden. However, Salmonella has developed several strategies to evade and modulate the host immune responses. In this sense, the main knowledge about the pathogenicity of this bacterium has been obtained by the study of mouse models with non-typhoidal serovars. However, this knowledge is not representative of all the pathologies caused by non-typhoidal serovars in the human. Here we review the most important features of typhoidal and non-typhoidal serovars and the diseases they cause in the human host, describing the virulence mechanisms used by these pathogens that have been identified in different models of infection.

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Phylogenetic analysis revealed that Salmonella Typhimurium ST313 isolated from humans and food in Brazil presented a high genomic similarity

Cited by 8 publications

References 60 publications

Phenotypic and genotypic characterization of Salmonella Typhimurium isolates from humans and foods in Brazil

Phenotypic and genotypic characterization of Salmonella Typhimurium isolates from humans and foods in Brazil

WGS-Based Lineage and Antimicrobial Resistance Pattern of Salmonella Typhimurium Isolated during 2000–2017 in Peru

New Insights on the Early Interaction Between Typhoid and Non-typhoid Salmonella Serovars and the Host Cells

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