Phylogenetic and Functional Characterization of Culturable Endophytic Actinobacteria Associated With Camellia spp. for Growth Promotion in Commercial Tea Cultivars

Borah, Atlanta; Thakur, Debajit

doi:10.3389/fmicb.2020.00318

Cited by 59 publications

(37 citation statements)

References 77 publications

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“…In this study, three actinobacterial strains were able to effectively produce endoglucanases. Similarly, several previous studies have reported that actinobacteria in the genera Actinoalloteichus, Actinoplanes, Amycolatopsis, Arthrobacter, Cellulomonas, Kitasatospora, Micromonospora, Mycobacterium, Nocardia, Nocardioides, Rhodococcus, Saccharomonospora, Streptomyces, Streptosporangium, and Thermomonospora could produce cell-wall degrading enzymes, especially endoglucanases [61,[64][65][66].…”

Section: Discussionmentioning

confidence: 69%

Isolation, Characterization, and Efficacy of Actinobacteria Associated with Arbuscular Mycorrhizal Spores in Promoting Plant Growth of Chili (Capsicum flutescens L.)

et al. 2021

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Nowadays, microorganisms that display plant growth promoting properties are significantly interesting for their potential role in reducing the use of chemical fertilizers. This research study proposed the isolation of the actinobacteria associated with arbuscular mycorrhizal fungi (AMF) spores and the investigation of their plant growth promoting properties in the in vitro assay. Three actinobacterial strains were obtained and identified to the genus Streptomyces (GETU-1 and GIG-1) and Amycolatopsis (GLM-2). The results indicated that all actinobacterial strains produced indole-3-acetic acid (IAA) and were positive in terms of siderophore, endoglucanase, and ammonia productions. In the in vitro assay, all strains were grown in the presence of water activity within a range of 0.897 to 0.998, pH values within a range of 5–11, and in the presence of 2.5% NaCl for the investigation of drought, pH, and salt tolerances, respectively. Additionally, all strains were able to tolerate commercial insecticides (propargite and methomyl) and fungicides (captan) at the recommended dosages for field applications. Only, Amycolatopsis sp. GLM-2 showed tolerance to benomyl at the recommended dose. All the obtained actinobacteria were characterized as plant growth promoting strains by improving the growth of chili plants (Capsicum flutescens L.). Moreover, the co-inoculation treatment of the obtained actinobacteria and AMF (Claroideoglomus etunicatum) spores could significantly increase plant growth, contribute to the chlorophyll index, and enhance fruit production in chili plants. Additionally, the highest value of AMF spore production and the greatest percentage of root colonization were observed in the treatment that had been co-inoculated with Streptomyces sp. GETU-1.

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Section: Discussionmentioning

confidence: 69%

Isolation, Characterization, and Efficacy of Actinobacteria Associated with Arbuscular Mycorrhizal Spores in Promoting Plant Growth of Chili (Capsicum flutescens L.)

et al. 2021

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“…Actinobacteria can promote plant growth by making nutrients/substrates (e.g., phosphorous, nitrogen, and iron) accessible to the host plant and producing various plant hormones ( Liu et al, 2017 ; Purushotham et al, 2018 ; Borah & Thakur, 2020 ). IAA is an important plant hormone beneficial to plant growth.…”

Section: Discussionmentioning

confidence: 99%

“…Evidence has shown that the root-associated actinobacteria contribute to plant growth promotion ( Dutta & Thakur, 2020 ), and plant stress alleviation ( Esmaeil Zade, Sadeghi & Moradi, 2018 ; Selim et al, 2019 ), through diverse mechanisms such as indole-3-acetic acid (IAA) biosynthesis, siderophore production, and phosphate solubilization. Studies have investigated the impact of actinobacteria on plant growth promotion and seed germination rate improvement ( Zamoum et al, 2015 ; Allali et al, 2019 ; Borah & Thakur, 2020 ). Also, actinobacteria ability to mitigate the negative impact of abiotic stress on the growth and physiology of crops, such as wheat ( Yandigeri et al., 2012 ; Li et al, 2019 ), maize ( Warrad et al, 2020 ), tomato ( Abbasi, Sadeghi & Safaie, 2020 ) has been investigated.…”

Section: Introductionmentioning

confidence: 99%

Diversity and function of culturable actinobacteria in the root-associated of Salvia miltiorrhiza Bunge

et al. 2021

PeerJ

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The root-associated actinobacteria play important roles in plant growth, nutrient use, and disease resistance due to their functional diversity. Salvia miltiorrhiza is a critical medicinal plant in China. The root actinobacterial community structure has been studied; however, the functions of root-associated actinobacteria of S. miltiorrhiza have not been elucidated. This study aimed to decipher the diversity and function of the culturable root-associated actinobacteria in plant growth using culture-dependent technology and culturable microbe metagenomes. We isolated 369 strains from the root-associated actinobacteria, belonging to four genera, among which Streptomyces was dominant. Besides, the functional prediction revealed some pathways related to plant growth, nitrogen and phosphorus metabolism, and antagonistic pathogens. We systematically described the diversity and functions of the culturable root-associated actinobacteria community. Our results demonstrated that the culturable root-associated actinobacteria of S. miltiorrhiza have rich functionalities, explaining the possible contribution of culturable root-associated actinobacteria to S. miltiorrhiza’s growth and development. This study provides new insights into understanding the function of the culturable root-associated actinobacteria and can be used as a knowledge base for plant growth promoters and biological control agent development in agriculture.

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“…As a primary example, polyketide synthases (PKSs) and nonribosomal peptide synthetases (NRPSs), which are multifunctional enzyme systems that assemble many of the secondary metabolites from simple building blocks including carboxylic acids and amino acids [202,203], show limited expression under laboratory conditions [204]. Extensive efforts have been made to unsilence such clusters [205,206]. Most genetic manipulation methods attempting to control PKSs and NRPSs as multifunctional enzymes to regulate expression of BGCs rely on multi-target approaches not specific to a single secondary metabolite and display complex interactions.…”

Section: Isolation Is the Problemmentioning

confidence: 99%

Deep learning approaches for natural product discovery from plant endophytic microbiomes

Aghdam

Brown

2021

Environmental Microbiome

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Plant microbiomes are not only diverse, but also appear to host a vast pool of secondary metabolites holding great promise for bioactive natural products and drug discovery. Yet, most microbes within plants appear to be uncultivable, and for those that can be cultivated, their metabolic potential lies largely hidden through regulatory silencing of biosynthetic genes. The recent explosion of powerful interdisciplinary approaches, including multi-omics methods to address multi-trophic interactions and artificial intelligence-based computational approaches to infer distribution of function, together present a paradigm shift in high-throughput approaches to natural product discovery from plant-associated microbes. Arguably, the key to characterizing and harnessing this biochemical capacity depends on a novel, systematic approach to characterize the triggers that turn on secondary metabolite biosynthesis through molecular or genetic signals from the host plant, members of the rich ‘in planta’ community, or from the environment. This review explores breakthrough approaches for natural product discovery from plant microbiomes, emphasizing the promise of deep learning as a tool for endophyte bioprospecting, endophyte biochemical novelty prediction, and endophyte regulatory control. It concludes with a proposed pipeline to harness global databases (genomic, metabolomic, regulomic, and chemical) to uncover and unsilence desirable natural products.

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Phylogenetic and Functional Characterization of Culturable Endophytic Actinobacteria Associated With Camellia spp. for Growth Promotion in Commercial Tea Cultivars

Cited by 59 publications

References 77 publications

Isolation, Characterization, and Efficacy of Actinobacteria Associated with Arbuscular Mycorrhizal Spores in Promoting Plant Growth of Chili (Capsicum flutescens L.)

Isolation, Characterization, and Efficacy of Actinobacteria Associated with Arbuscular Mycorrhizal Spores in Promoting Plant Growth of Chili (Capsicum flutescens L.)

Diversity and function of culturable actinobacteria in the root-associated of Salvia miltiorrhiza Bunge

Deep learning approaches for natural product discovery from plant endophytic microbiomes

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