Phylogenetic and functional diversity of aldehyde-alcohol dehydrogenases in microalgae

Lis, Robert van; Couté, Yohann; Brugière, Sabine; Tourasse, Nicolas J.; Laurent, Benoist; Nitschke, Wolfgang; Vallon, Olivier; Atteia, Ariane

doi:10.1007/s11103-020-01105-9

Cited by 7 publications

(4 citation statements)

References 69 publications

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“…The availability of an annotated genome sequence of Polytomella sp. (van Lis et al, 2021) allowed a global proteomics approach to decipher its metabolic pathways involved in the assimilation of butyrate. Cells grown on either acetate or butyrate, at a fixed C concentration of 1.0 g C .L -1 (Fig.…”

Section: Resultsmentioning

confidence: 99%

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Proteomics unveil a central role for peroxisomes in butyrate assimilation of the heterotrophic Chlorophyte alga Polytomella sp

Lacroux

Atteia

Brugière

et al. 2022

Preprint

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Volatile fatty acids found in effluents of the dark fermentation of biowastes can be used for mixotrophic growth of microalgae, improving productivity and reducing the cost of the feedstock. Microalgae can use the acetate in the effluents very well, but butyrate is poorly assimilated and can inhibit growth above 1 gC.L-1. The non-photosynthetic chlorophyte alga Polytomella sp. SAG 198.80 was found to be able to assimilate butyrate fast. To decipher the metabolic pathways implicated in butyrate assimilation, quantitative proteomics study was developed comparing Polytomella sp. cells grown on acetate and butyrate at 1 gC.L-1. After statistical analysis, a total of 1772 proteins were retained, of which 119 proteins were found to be overaccumulated on butyrate vs. only 46 on acetate, indicating that butyrate assimilation necessitates additional metabolic steps. The data show that butyrate assimilation occurs in the peroxisome via the beta-oxidation pathway to produce acetyl-CoA and further tri/dicarboxylic acids in the glyoxylate cycle. Concomitantly, reactive oxygen species defense enzymes as well as the branched amino acid degradation pathway were strongly induced. Although no clear dedicated butyrate transport mechanism could be inferred, several membrane transporters induced on butyrate are identified as potential condidates. Metabolic responses correspond globally to the increased needs for central cofactors NAD, ATP and CoA, especially in the peroxisome and the cytosol.

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Section: Resultsmentioning

confidence: 99%

“…The assembly and structural annotation of the genome sequence of Polytomella sp. was described previously (van Lis et al, 2021). The predicted proteins (genome to be released at an later date) were used for the construction of the database for proteomics analysis.…”

Section: Methodsmentioning

confidence: 99%

Proteomics unveil a central role for peroxisomes in butyrate assimilation of the heterotrophic Chlorophyte alga Polytomella sp

Lacroux

Atteia

Brugière

et al. 2022

Preprint

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show abstract

“…The assembly and structural annotation of the genome sequence of Polytomella sp. was described previously ( van Lis et al, 2021 ). The predicted proteins 1 were used for the construction of the database for proteomics analysis.…”

Section: Methodsmentioning

confidence: 99%

“…The exclusively heterotrophic chlorophyte Polytomella sp. SAG 198.80 was chosen as the model species since, besides the availability of an annotated genome sequence ( van Lis et al, 2021 ), it is to our knowledge the alga for which the fastest butyrate assimilation has been described ( Wise, 1955 , 1959 ), and most recently by the authors of this work ( Lacroux et al, 2022 ). The genus Polytomella belongs to the Reinhardtinia clade of Volvocine algae ( Craig et al, 2021 ) and has diverged from a Chlamydomonas -like ancestor after having lost photosynthesis along with the chloroplast genome ( Smith and Lee, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Proteomics unveil a central role for peroxisomes in butyrate assimilation of the heterotrophic Chlorophyte alga Polytomella sp.

Lacroux

Atteia²,

Brugière³

et al. 2022

Front. Microbiol.

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Volatile fatty acids found in effluents of the dark fermentation of biowastes can be used for mixotrophic growth of microalgae, improving productivity and reducing the cost of the feedstock. Microalgae can use the acetate in the effluents very well, but butyrate is poorly assimilated and can inhibit growth above 1 gC.L−1. The non-photosynthetic chlorophyte alga Polytomella sp. SAG 198.80 was found to be able to assimilate butyrate fast. To decipher the metabolic pathways implicated in butyrate assimilation, quantitative proteomics study was developed comparing Polytomella sp. cells grown on acetate and butyrate at 1 gC.L−1. After statistical analysis, a total of 1772 proteins were retained, of which 119 proteins were found to be overaccumulated on butyrate vs. only 46 on acetate, indicating that butyrate assimilation necessitates additional metabolic steps. The data show that butyrate assimilation occurs in the peroxisome via the β-oxidation pathway to produce acetyl-CoA and further tri/dicarboxylic acids in the glyoxylate cycle. Concomitantly, reactive oxygen species defense enzymes as well as the branched amino acid degradation pathway were strongly induced. Although no clear dedicated butyrate transport mechanism could be inferred, several membrane transporters induced on butyrate are identified as potential condidates. Metabolic responses correspond globally to the increased needs for central cofactors NAD, ATP and CoA, especially in the peroxisome and the cytosol.

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Metabolic networks during dark anoxia

Posewitz¹,

Atteia²,

Hemschemeier³

et al. 2023

The Chlamydomonas Sourcebook

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Phylogenetic and functional diversity of aldehyde-alcohol dehydrogenases in microalgae

Cited by 7 publications

References 69 publications

Proteomics unveil a central role for peroxisomes in butyrate assimilation of the heterotrophic Chlorophyte alga Polytomella sp

Proteomics unveil a central role for peroxisomes in butyrate assimilation of the heterotrophic Chlorophyte alga Polytomella sp

Proteomics unveil a central role for peroxisomes in butyrate assimilation of the heterotrophic Chlorophyte alga Polytomella sp.

Metabolic networks during dark anoxia

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