Phylogenetic and genomic analyses of two new species of Clavispora (Metschnikowiaceae, Saccharomycetales) from Central China

Abstract

Species in the genus Clavispora have previously been reported primarily in the northeast and northwest regions of China; the species diversity of Clavispora in central China is not currently clear. In this study, phylogenetic inferences of Clavispora based on sequences of a single-locus (LSU D1/D2) and a two-locus (LSU D1/D2 and ITS) were conducted. Two new species isolated from rotting wood in central China, namely Clavispora xylosa sp. nov. and Clavispora paralusitaniae sp. nov., were delimited and proposed based on morphological and molecular evidence. Cl. xylosa was closely related to C. thailandica CBS 10610T, but with 11.5% divergence in the LSU D1/D2 domains and 11.5% divergence in the ITS regions. Cl. paralusitaniae was a sister to Cl. lusitaniae CBS 6936T from which it differs with 4.7% divergence in the LSU D1/D2 domains and 5.4% divergence in the ITS regions. Description of Cl. xylosa sp. nov. and Cl. paralusitaniae sp. nov. was also supported by morphological comparisons and genomic analyses between the two new species and their closest relatives, C. thailandica CBS 10610T and Cl. lusitaniae CBS 6936T. These results indicate a potentially great diversity of Clavispora spp. inhabiting rotting wood in central China, ripe for future discovery.

Diversity and distribution of yeasts in intertidal zones of China

“…In this study, we obtained 286 yeast species, including 140 ascomycetous and 146 basidiomycetous species associated with intertidal zones of China (Table S2). Boekhout et al (2022) summarized that at least 782 yeast species have been recorded from China recorded until 2020 and 49 additional yeast species were reported from China in the past three years (Gao et al, 2021;Shi et al, 2021;Chai et al, 2022a;Chai et al, 2022b;Chai et al, 2022c;Chu et al, 2022;Li et al, 2022;Wei et al, 2022;Chai et al, 2023;Liu et al, 2023;Qiao et al, 2023;Yu et al, 2023;Zhu et al, 2023a;Zhu et al, 2023b). The number of yeast species discovered in this study accounts for 34.7% in the total yeast species that have been found from China, indicating that there are abundant resources of culturable yeasts in the intertidal zones of China.…”