Phylogenetic and recombination analysis of coronavirus HKU1, a novel coronavirus from patients with pneumonia

Woo, Patrick C. Y.; Lau, Susanna K. P.; Huang, Ying; Tsoi, Hoi-Wah; Chan, Kwok‐Hung; Yuen, Kwok‐Yung

doi:10.1007/s00705-005-0573-2

Cited by 52 publications

(49 citation statements)

References 24 publications

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“…In addition, there are at least 3 genotypes with recombination between the different genotypes (11,12). Multiple-alignment comparison with other group II coronavirus spike proteins demonstrated that the HCoV-HKU1 spike displays strong similarities to a class I virus fusion protein with a potential cleavage site between residues 760 and 761, which may be processed by a membrane bound, calcium-dependent resident Golgi protease such as furin.…”

Section: Introductionmentioning

confidence: 99%

RETRACTED: Spike Protein, S, of Human Coronavirus HKU1: Role in Viral Life Cycle and Application in Antibody Detection

Chan

Woo

Lau

et al. 2008

Exp Biol Med (Maywood)

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We recently described the discovery, genome, clinical features, genotypes and evolution of a novel and global human respiratory virus named human coronavirus HKU1 (HCoV-HKU1) which is not yet culturable. We expressed a C-terminal FLAG-tagged CoV-HKU1 spike (S) protein by the Semliki Forest Virus (SFV) system and investigated its maturation profile. Pulse chase labeling revealed that S-FLAG was expressed as high-mannose N-glycans of monomers and trimers. It was predominantly cleaved into subdomains S1 and S2 during maturation. S1 was secreted into the medium. Immunofluorescence analysis visualized S along the secretory pathway from endoplasmic reticulum to plasma membrane. Cleavage of S and release of HCoV-HKU1 S pseudotyped virus were inhibited by furin or furin-like enzyme inhibitors. The cell-based expressed full-length S-FLAG could be recognized by the convalescent serum obtained from a patient with HCoV-HKU1 pneumonia. The data suggest that the native form of HCoV-HKU1 spike expressed in our system can be used in developing serological diagnostic assay and in understanding the role of S in the viral life cycle.

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Section: Introductionmentioning

confidence: 99%

RETRACTED: Spike Protein, S, of Human Coronavirus HKU1: Role in Viral Life Cycle and Application in Antibody Detection

Chan

Woo

Lau

et al. 2008

Exp Biol Med (Maywood)

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“…Recombination events have been observed for different genotypes of HKU1, in which recombination breakpoints were found on genotypes B and C in the nsp6-nsp7 and nsp16-HE genes, respectively [70] (Figure 4). HKU1 was also shown to be able to recombine with other animal beta-CoVs, such as MHV, at the nsp3, nsp4, nsp6, nsp7, nps9, and nsp10 genes [77] (Figure 4). [78].…”

Section: Genetic Recombination In Human Coronavirusesmentioning

confidence: 99%

Epidemiology, Genetic Recombination, and Pathogenesis of Coronaviruses

Wong

Shi

et al. 2016

Trends in Microbiology

2,656

2,634

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Human coronaviruses (HCoVs) were first described in the 1960s for patients with the common cold. Since then, more HCoVs have been discovered, including those that cause severe acute respiratory syndrome (SARS) and Middle East respiratory syndrome (MERS), two pathogens that, upon infection, can cause fatal respiratory disease in humans. It was recently discovered that dromedary camels in Saudi Arabia harbor three different HCoV species, including a dominant MERS HCoV lineage that was responsible for the outbreaks in the Middle East and South Korea during 2015. In this review we aim to compare and contrast the different HCoVs with regard to epidemiology and pathogenesis, in addition to the virus evolution and recombination events which have, on occasion, resulted in outbreaks amongst humans.

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“…Although the majority of these infections are selflimited and mild, severe infections can occur mainly in young children, elderly, immunosupressed individuals, and patients with underlying diseases. Influenza (Flu) A and B viruses, respiratory syncytial virus (RSV), parainfluenzaviruses (PIV), adenovirus (ADV), rhinovirus (RhV), enterovirus (EV), and human coronavirus (CoV) OC43 and 229E are classical respiratory pathogens associated with ARI (Atmar et al, 2012;Creer et al, 2006;Dimopoulos et al, 2012;Henderson et al, 1979;Jennings et al, 2008;Ruuskanen et al, 2011;Yun et al, 1995) as well as other currently described viruses such as human metapneumovirus (hMPV) (Van den Hoogen et al, 2004), SARS-CoV (Ksiazek et al, 2003), CoV NL63 ( Van der Hoek et al, 2004) and HKU1 (Woo et al, 2005), MERS-CoV (Zaki et al, 2012), human bocavirus (BoV) (Allander et al, 2005), and others (Ruuskanen et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Analytical performance of the automated multianalyte point-of-care mariPOC® for the detection of respiratory viruses

Sanbonmatsu‐Gámez

Pérez-Ruiz

Lara-Oya

et al. 2015

Diagnostic Microbiology and Infectious Disease

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The analytical performance of mariPOC® respi test (ArcDia® Laboratories, Turku, Finland) was evaluated using nucleic acid amplification techniques (NAATs) as the gold standard. The mariPOC assay allows automated detection of antigens from 8 respiratory viruses: influenza A and B viruses, respiratory syncytial virus, adenovirus, human metapneumovirus, and parainfluenza viruses 1-3. Positive results from samples with high viral load are available in 20min. Nasopharyngeal aspirates (n=192) from patients with acute respiratory infection and from previously positive samples were analyzed by mariPOC and NAATs (Simplexa(TM) FluA/FluB & RSV kit [n=118] and Luminex® Respiratory virus panel xTAG® RVP FAST [n=74]). Sensitivity, specificity, positive predictive value, and negative predictive value of mariPOC were 85.4%, 99.2%, 95.9%, and 97%, respectively, and 84.6% of positive results were reported in 20min. The good analytical performance and extended portfolio of mariPOC show this rapid assay as a good alternative for the etiological diagnosis of acute respiratory infection in laboratories that are not equipped with molecular assays.

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Phylogenetic and recombination analysis of coronavirus HKU1, a novel coronavirus from patients with pneumonia

Cited by 52 publications

References 24 publications

RETRACTED: Spike Protein, S, of Human Coronavirus HKU1: Role in Viral Life Cycle and Application in Antibody Detection

RETRACTED: Spike Protein, S, of Human Coronavirus HKU1: Role in Viral Life Cycle and Application in Antibody Detection

Epidemiology, Genetic Recombination, and Pathogenesis of Coronaviruses

Analytical performance of the automated multianalyte point-of-care mariPOC® for the detection of respiratory viruses

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