Phylogenetic characterization of bioemulsifier-producing bacteria

Franzetti, Andrea; Gandolfi, Isabella; Bertolini, Valentina; Raimondi, Chiara; Piscitello, Marco; Papacchini, Maddalena; Bestetti, Giuseppina

doi:10.1016/j.ibiod.2011.01.014

Cited by 15 publications

(7 citation statements)

References 29 publications

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“…Exotic bacteria must overcome the stability of the soil bacterial community to survive in a new habitat. In this study, we observed that the enriched exotic bacteria often dominated in soils whose environmental characteristics were the same as those of the recipient soils, including members of the genera Gaiella (Eo et al, 2015), Nocardioides (Topp et al, 2000), Arthrobacter (Aislabie et al, 2005), Gp6 (Pérez-Leblic et al, 2012), Gp16 (Pérez-Leblic et al, 2012), Geminicoccus (Franzetti et al, 2011), and Micromonospora (Mcalpine et al, 2008).…”

Section: Discussionmentioning

confidence: 85%

“…In contrast, the OintoA-treated soils exhibited increases in the abundances of Pseudomonas , Nocardioides , Prevotella , Arthrobacter , Gp6 , Gp16 , Gaiella , Geminicoccus , and Micromonospora . Moreover, the abundances of five genera, Gp6 , Gp16 , Gaiella , Geminicoccus , and Micromonospora , which have been reported as indigenous bacteria in aromatic and organic compound-contaminated sites (Mcalpine et al, 2008; Franzetti et al, 2011; Pérez-Leblic et al, 2012), increased in the soils in response to the OintoA treatment. Additionally, while Prevotella and Gaiella OTUs were rare in both ONS and ACS soil, both OTUs were abundant in the OintoA and AintoO treatments, respectively (Figure 1B).…”

Section: Resultsmentioning

confidence: 97%

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Non-synchronous Structural and Functional Dynamics During the Coalescence of Two Distinct Soil Bacterial Communities

et al. 2019

Front. Microbiol.

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Soil is a unique environment in which the microbiota is frequently subjected to community coalescence. Additions of organic fertilizer and precipitation of dust induce coalescent events in soil. However, the fates of these communities after coalescence remain uncharted. Thus, to explore the effects of microbiota coalescence, we performed reciprocal inoculation and incubation experiments in microcosms using two distinct soils. The soils were, respectively, collected from a cropland and an industrial site, and the reciprocal inoculation was performed as models for the incursion of highly exotic microbiota into the soil. After incubation under either aerobic or anaerobic conditions for two months, the soils were assayed for their bacterial community structure and denitrification function. According to the 16S rRNA gene sequencing results, the inoculated soil showed a significant shift in bacterial community structure after incubation—particularly in the industrial soil. The structures of the bacterial communities changed following the coalescence but were predicted to have the same functional potential, e.g., nitrogen metabolism, as determined by the quantification of denitrifying genes and nitrogen gas production in the inoculated soil samples, which showed values equivalent those in the original recipient soil samples regardless of inoculum used. The functional prediction based on the known genomes of the taxa that shifted in the incubated sample communities indicates that the high functional overlap and redundancy across bacteria acted as a mechanism that preserved all the metabolic functions in the soil. These findings hint at the mechanisms underlying soil biodiversity maintenance and ecosystem function.

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Section: Discussionmentioning

confidence: 85%

Section: Resultsmentioning

confidence: 97%

Non-synchronous Structural and Functional Dynamics During the Coalescence of Two Distinct Soil Bacterial Communities

et al. 2019

Front. Microbiol.

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“…However, the biotechnological potential of this genus as a marine bioemulsifier producer has only been reported recently [8,36]. The occurrence of bioemulsifier-producing Microbacterium species in intertidal environments is not surprising, given the metabolic versatility exhibited by this genus; some of them can metabolize hydrocarbons, presumably through bioemulsifier synthesis [38,39]. In addition, the availability of hydrophobic substrates in coastal marine environments may select for bacteria capable of synthesizing surface-active molecules to enhance nutrient uptake [1].…”

Section: Resultsmentioning

confidence: 99%

Emulsifying Activity and Stability of a Non-Toxic Bioemulsifier Synthesized by Microbacterium sp. MC3B-10

Camacho-Chab

Guézennec

Chan-Bacab

et al. 2013

IJMS

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A previously reported bacterial bioemulsifier, here termed microbactan, was further analyzed to characterize its lipid component, molecular weight, ionic character and toxicity, along with its bioemulsifying potential for hydrophobic substrates at a range of temperatures, salinities and pH values. Analyses showed that microbactan is a high molecular weight (700 kDa), non-ionic molecule. Gas chromatography of the lipid fraction revealed the presence of palmitic, stearic, and oleic acids; thus microbactan may be considered a glycolipoprotein. Microbactan emulsified aromatic hydrocarbons and oils to various extents; the highest emulsification index was recorded against motor oil (96%). The stability of the microbactan-motor oil emulsion model reached its highest level (94%) at 50 °C, pH 10 and 3.5% NaCl content. It was not toxic to Artemia salina nauplii. OPEN ACCESS Int. J. Mol. Sci. 2013, 14 18960Microbactan is, therefore, a non-toxic and non-ionic bioemulsifier of high molecular weight with affinity for a range of oily substrates. Comparative phylogenetic assessment of the 16S rDNA gene of Microbacterium sp. MC3B-10 with genes derived from other marine Microbacterium species suggested that this genus is well represented in coastal zones. The chemical nature and stability of the bioemulsifier suggest its potential application in bioremediation of marine environments and in cosmetics.

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“…Bottles were then incubated at 30 • C, refreshed several times, and pure cultures were isolated by plating on LB agar medium. A fragment of the gene 16S rRNA was PCR-amplified using Com primers [11] as previously reported [12]. The Ribosomal Database Project (RDP) classifier [13] was used for taxonomic assignments of sequences.…”

Section: Inoculum Selectionmentioning

confidence: 99%

“…The Ribosomal Database Project (RDP) classifier [13] was used for taxonomic assignments of sequences. The cultures were then incubated 24 h in LB rich medium at 30 • C under shaking, washed and resuspended to OD 540 = 0.1 [12]. Subsequently the degradation capabilities of isolates were screened by liquid growth experiment.…”

Section: Inoculum Selectionmentioning

confidence: 99%

Lab-scale tests and numerical simulations for in situ treatment of polluted groundwater

Careghini

Saponaro

Sezenna

et al. 2015

Journal of Hazardous Materials

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Phylogenetic characterization of bioemulsifier-producing bacteria

Cited by 15 publications

References 29 publications

Non-synchronous Structural and Functional Dynamics During the Coalescence of Two Distinct Soil Bacterial Communities

Non-synchronous Structural and Functional Dynamics During the Coalescence of Two Distinct Soil Bacterial Communities

Emulsifying Activity and Stability of a Non-Toxic Bioemulsifier Synthesized by Microbacterium sp. MC3B-10

Lab-scale tests and numerical simulations for in situ treatment of polluted groundwater

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