Phylogenetic Comparison of Leucocytozoon spp. from Wild Birds of Japan

Sato, Yukita; Hagihara, Mio; Yamaguchi, Tsuyoshi; Yukawa, Masayoshi; Murata, Koichi

doi:10.1292/jvms.69.55

Cited by 18 publications

(13 citation statements)

References 22 publications

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“…The reactions using BigDye TM terminator mix (Applied Biosystems, Foster City, California, U.S.A.), were run on an ABI3130 (Applied Biosystems, Foster City, California, U.S.A.) auto sequencer. Phylogenetic analyses of about 478 bp sequences were performed as described previously [34]. Briefly, using the neighbor-joining (NJ) method by PAUP program, the Kimura two-parameter model was utilized to estimate the evolutionary distances.…”

Section: Methodsmentioning

confidence: 99%

“…Following the former studies on the prevalence of avian blood protozoa in Japan [11,25], further molecular biological analyses of avian Plasmodium and Leucocytozoon have been reported on wild birds of Japan, respectively [27,34]. More recently, Murata et al (2008) also showed that avian malaria distributed even in a particularly isolated environment of an oceanic island, Minami Daito Island, Japan [26].…”

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Detection of Avian Plasmodium spp. DNA Sequences from Mosquitoes Captured in Minami Daito Island of Japan

Ejiri

Sato

Sasaki

et al. 2008

The Journal of Veterinary Medical Science

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ABSTRACT. Several species of birds in Minami Daito Island, an oceanic island located in the far south from the main islands of Japan, were found to be infected with avian Plasmodium. However, no vector species of the avian malaria in this island have been revealed yet. To speculate potential vectors, we collected mosquitoes there and investigated using a PCR procedure whether the mosquitoes harbor avian malaria or not. Totally 1,264 mosquitoes including 9 species were collected during March 2006 to February 2007. The mosquitoes collected were stored every species, sampled date and location for DNA extraction. Fifteen out of 399 DNA samples showed positive for the partial mtDNA cytb gene of avian Plasmodium. Estimated minimum infection rate among collected mosquitoes was 1.2% in this study. Four species of mosquitoes; Aedes albopictus, Culex quinquefasciatus, Lutzia fuscanus and Mansonia sp. had avian Plasmodium gene sequences. Detected DNA sequences from A. albopictus and L. fuscanus were identical to an avian Plasmodium lineage detected in bull-headed shrike (Lanius bucephalus) captured in the island. Different sequences were detected from C. quinquefasciatus, which were corresponding to an avian Plasmodium from a sparrow (Passer montanus) and Plasmodium gallinaceum. Our results suggest that A. albopictus, Lutzia fuscanus, C. quinquefasciatus, and Mansonia sp. could be potential vectors of avian malaria in Minami Daito Island. This study was the first report of molecular detection of avian Plasmodium from mosquitoes in Japan.

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Section: Methodsmentioning

confidence: 99%

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Detection of Avian Plasmodium spp. DNA Sequences from Mosquitoes Captured in Minami Daito Island of Japan

Ejiri

Sato

Sasaki

et al. 2008

The Journal of Veterinary Medical Science

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“…and/or Haemoproteus spp., and LcytbF and LcytbR were used for Leucocytozoon spp. [28]. We also used the primer set of HAEMNFI and HAEMNR3 [9] for the first reaction and HAEMF and HAEMR2 [2] for the second to amplify Plasmodium spp.…”

Section: Sample Collection Of Birdsmentioning

confidence: 99%

Molecular Identification of Avian Haemosporidia in Wild Birds and Mosquitoes on Tsushima Island, Japan

Tanigawa

Sato

Ejiri

et al. 2013

The Journal of Veterinary Medical Science

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ABSTRACT. We investigated for the first time the prevalence of avian haemosporidia of genera Plasmodium, Haemoproteus and Leucocytozoon among birds and mosquitoes on Tsushima Island of Japan, which is located between Japan and the Korean Peninsula. Of 55 wild birds belonging to 33 species, 16 (29.1%) tested positive for haemosporidia as follows: Plasmodium spp. (11/55; 20.0%); Haemoproteus spp. (2/55; 3.6%); and Leucocytozoon spp. (3/55; 5.5%). A genetic lineage isolated from the Eurasian Sparrowhawk (Accipiter nisus) was identical to that of the known avian malaria parasite P. circumflexum. Several genetic lineages were identical or closely related to the parasite lineages that were previously detected in birds and mosquitoes in Japan and Korea. Another single identical genetic lineage was also detected in both migratory and resident birds. A total of 753 mosquitoes from 12 species were collected; and one fully fed Aedes albopictus was positive for avian Plasmodium(1/753; 0.13%) which is identical to a genetic lineage detected in both mosquitoes in Japan and birds in Korea. Blood-meal identifications of blood-fed mosquitoes showed direct contact between the mosquitoes and 4 species of mammals including humans, cattle, rodents and the endangered Tsushima leopard cat (Prionailurus bengalensis euptilura). Migratory birds use Tsushima Island as a site for wintering, breeding and resting, and our results suggest the transmission of avian haematozoa between resident and migratory birds during their stay on Tsushima Island.

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“…Although at least 35 morphologically defined species have been described to date [ 1 ], molecular studies suggest that the true diversity well exceeds this number e.g. [ 2 – 4 ]. Several of the molecular lineages have demonstrated that the degree of host-specificity varies; some lineages are found consistently only in one bird species, whereas other lineages develop successfully in a wide range of taxonomically varied hosts [ 2 , 3 ].…”

Section: Introductionmentioning

confidence: 99%

Molecular analyses on host-seeking black flies (Diptera: Simuliidae) reveal a diverse assemblage of Leucocytozoon (Apicomplexa: Haemospororida) parasites in an alpine ecosystem

et al. 2015

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BackgroundMolecular studies have suggested that the true diversity of Leucocytozoon (Apicomplexa: Haemospororida) species well exceeds the approximately 35 currently described taxa. Further, the degree of host-specificity may vary substantially among lineages. Parasite distribution can be influenced by the ability of the parasite to infect a host, vector preferences for certain avian hosts, or other factors such as microhabitat requirements that increase the probability that vertebrate hosts and vectors are in frequent contact with each other. Whereas most studies of haemosporidians have focused on passerine hosts, sampling vectors in the same habitats may allow the detection of other lineages affecting other hosts.MethodsWe sampled abundant, ornithophilic black flies (Simuliidae) across a variety of sites and habitats in the Colorado Rocky Mountains throughout the summer of 2007. Black flies were screened with PCR using Leucocytozoon-specific primers that amplify a portion of the cytochrome b gene, and the sequences were compared to the haplotypes in the MalAvi database. Infections of Leucocytozoon from birds sampled in the same area were also included.ResultsWe recovered 33 unique haplotypes from the black flies in this study area, which represented a large phylogenetic diversity of Leucocytozoon parasites. However, there were no clear patterns of avian host species or geography for the distribution of Leucocytozoon haplotypes in the phylogeny.ConclusionsSampling host-seeking vectors is a useful way to obtain a wide variety of avian haemosporidian haplotypes from a given area and may prove useful for understanding the global patterns of host, parasite, and vector associations of these ubiquitous and diverse parasites.Electronic supplementary materialThe online version of this article (doi:10.1186/s13071-015-0952-9) contains supplementary material, which is available to authorized users.

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Phylogenetic Comparison of Leucocytozoon spp. from Wild Birds of Japan

Cited by 18 publications

References 22 publications

Detection of Avian Plasmodium spp. DNA Sequences from Mosquitoes Captured in Minami Daito Island of Japan

Detection of Avian Plasmodium spp. DNA Sequences from Mosquitoes Captured in Minami Daito Island of Japan

Molecular Identification of Avian Haemosporidia in Wild Birds and Mosquitoes on Tsushima Island, Japan

Molecular analyses on host-seeking black flies (Diptera: Simuliidae) reveal a diverse assemblage of Leucocytozoon (Apicomplexa: Haemospororida) parasites in an alpine ecosystem

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