Phylogenetic confirmation of the genus<i>Robbea</i>(Nematoda: Desmodoridae, Stilbonematinae) with the description of three new species

Ott, Jörg; Gruber‐Vodicka, Harald R.; Leisch, Nikolaus; Zimmermann, Judith

doi:10.1080/14772000.2014.941038

Cited by 9 publications

(17 citation statements)

References 41 publications

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“…; Ott et al . ). All stilbonematine species from the same genus formed highly supported clades independent of their sampling location (Fig.…”

Section: Resultsmentioning

confidence: 97%

“…Cycling conditions were as described in Ott et al . (). The purified host PCR products were sequenced bidirectionally with internal forward and reverse primers listed in Table S3 (Supporting information).…”

Section: Methodsmentioning

confidence: 97%

“…(e.g. Meldal et al 2007;Van Megen et al 2009;Ott et al 2014). For the symbionts, we sequenced the partial 16S-23S rRNA operon, including the 16S rRNA gene, the internal transcribed spacer (ITS) between the 16S and 23S rRNA genes and the first 200 bp of the 23S rRNA, because the 16S rRNA gene alone did not provide sufficient resolution for relationships within some clades (e.g.…”

Section: Dna Extraction Pcr and Sequencingmentioning

confidence: 99%

“…Genes were amplified by PCR with Phusion â DNA polymerase (Finnzymes, Finland) and the primers listed in Table S3 (Supporting information). Cycling conditions were as described in Ott et al (2014). The purified host PCR products were sequenced bidirectionally with internal forward and reverse primers listed in Table S3 (Supporting information).…”

Section: Dna Extraction Pcr and Sequencingmentioning

confidence: 99%

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Closely coupled evolutionary history of ecto‐ and endosymbionts from two distantly related animal phyla

et al. 2016

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The level of integration between associated partners can range from ectosymbioses to extracellular and intracellular endosymbioses, and this range has been assumed to reflect a continuum from less intimate to evolutionarily highly stable associations. In this study, we examined the specificity and evolutionary history of marine symbioses in a group of closely related sulphur-oxidizing bacteria, called Candidatus Thiosymbion, that have established ecto-and endosymbioses with two distantly related animal phyla, Nematoda and Annelida. Intriguingly, in the ectosymbiotic associations of stilbonematine nematodes, we observed a high degree of congruence between symbiont and host phylogenies, based on their ribosomal RNA (rRNA) genes. In contrast, for the endosymbioses of gutless phallodriline annelids (oligochaetes), we found only a weak congruence between symbiont and host phylogenies, based on analyses of symbiont 16S rRNA genes and six host genetic markers. The much higher degree of congruence between nematodes and their ectosymbionts compared to those of annelids and their endosymbionts was confirmed by cophylogenetic analyses. These revealed 15 significant codivergence events between stilbonematine nematodes and their ectosymbionts, but only one event between gutless phallodrilines and their endosymbionts. Phylogenetic analyses of 16S rRNA gene sequences from 50 Cand. Thiosymbion species revealed seven well-supported clades that contained both stilbonematine ectosymbionts and phallodriline endosymbionts. This closely coupled evolutionary history of marine ecto-and endosymbionts suggests that switches between symbiotic lifestyles and between the two host phyla occurred multiple times during the evolution of the Cand. Thiosymbion clade, and highlights the remarkable flexibility of these symbiotic bacteria.

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“…; Ott et al . ). All stilbonematine species from the same genus formed highly supported clades independent of their sampling location (Fig.…”

Section: Resultsmentioning

confidence: 97%

Section: Methodsmentioning

confidence: 97%

Section: Dna Extraction Pcr and Sequencingmentioning

confidence: 99%

Section: Dna Extraction Pcr and Sequencingmentioning

confidence: 99%

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Closely coupled evolutionary history of ecto‐ and endosymbionts from two distantly related animal phyla

et al. 2016

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“…Two supposed sequences of Eubostrichus topiarius (JN968219 and JN968237), in particular, are out of place, as another sequence of the same species (Y16917) is found in a separate clade together with other Eubostrichus species. Likewise, one sequence of Robbea hypermnestra (KJ414467; Ott et al, 2014) is located amongst other Stilbonematinae sequences, whereas another sequence of the same species identified by the same laboratory (Y16921; Kampfer, Sturmbauer & Ott, 1998) is grouped with species of Spiriniinae. The anomalous placement of the latter species is unlikely to be a result of misidentification, and may be the result of inherent artefacts in phylogenetic tree routines (Altaba, 2009).…”

Section: Relationships Within the Desmodoroidea Based On D2-d3 And Ssmentioning

confidence: 99%

Phylogenetic relationships within the superfamily Desmodoroidea (Nematoda: Desmodorida), with descriptions of two new and one known species

Leduc

Zhao

2016

Zool J Linn Soc

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Three nematode species of the superfamily Desmodoroidea Filipjev, 1922, were isolated from beach sediments in Wellington, New Zealand, for morphological and molecular analyses. Two of these species, Desmodorella verscheldei sp. nov. and Dracograllus ngakei sp. nov., were new to science and are described herein. Epsilonema rugatum Lorenzen, 1973, comb. nov., which was originally described from New Zealand material as a subspecies of Epsilonema dentatum from Chile, is redescribed and elevated to the rank of species based on cuticular ornamentation. The phylogenetic relationships amongst the three Desmodoroidea families are investigated based on new and existing sequences of the D2 and D3 expansions segments of large subunit (LSU) 28S rRNA gene and small subunit (SSU) of 18S rDNA gene. Our analyses suggest that the Draconematidae is a sister taxon to the Desmodorinae and Spiriniinae, with the Draconematidae forming a monophyletic crown group and the Desmodorinae and Spiriniinae forming a paraphyletic stem group. Phylogenetic relationships between the Epsilonematidae and Stilbonematinae, however, could not be determined with certainty. The SSU and D2-D3 of LSU consensus trees indicate that the morphological resemblance between the Draconematidae and Epsilonematidae, which are both characterized by swollen pharyngeal body regions and mid-posterior body regions with specialized setae, reflects distinct and independently evolved adaptations to their unusual mode of locomotion, with differences in the structure and distribution of specialized setae between the two families also consistent with convergent evolution. We show that the family Desmodoridae and superfamily Desmodoroidea as currently defined are not monophyletic. It was not possible to determine whether the Prodesmodorinae are more closely related to the Desmodoroidea or Microlaimoidea, although it is clear that they do not belong to the Desmodoridae. The single Molgolaiminae sequence available formed a distinct clade together with the superfamily Microlaimoidea, and should therefore be placed with the latter. Clarifying the phylogenetic relationships within the Desmodoroidea will require greater focus on the Pseudonchinae, Molgolaiminae, and Epsilonematidae, for which no or very few sequences are available at present.

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Monitoring shallow methane‐derived authigenic carbonate: Insights from a UK Marine Protected Area

Noble-James

Judd

Diesing

et al. 2020

Aquatic Conservation

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Methane‐derived authigenic carbonate (MDAC) is a biogenic rocky substrate formed by microbial assemblages below the seabed. It performs important ecosystem functions, including the provision of reef‐like habitats on soft sediments and the sequestration of carbon. The protection of MDAC is limited at the global scale; however, 27 marine protected areas (MPAs) have been designated in European waters for ‘cold seep’ MDAC, mainly in shallow waters (<200 m). Few studies have been conducted on these habitats from a conservation perspective. The effective management of MDAC structures requires an understanding of their ecology and physical characteristics in a healthy condition. This is best achieved using a multidisciplinary approach to provide evidence on predefined aspects of MDAC structures, termed feature attributes, which can be assessed to determine habitat condition over time. This article presents the first UK effort to develop a multidisciplinary approach to monitoring shallow MDAC feature attributes, using the Croker Carbonate Slabs Special Area of Conservation (SAC) as a case study. A range of remote and physical survey methods were used to characterize the MDAC and associated relatively unimpacted and healthy biological communities. The data confirm that the SAC contains the largest known area of shallow MDAC in European waters, that methane release is ongoing, and that MDAC is still likely to be forming. Specialized chemoautotrophic fauna were not recorded, possibly due to the dominance of fauna that derive carbon (ultimately) from photosynthesis. Five epifaunal taxa were found to be associated with MDAC, but not with the surrounding sediments. The broad multidisciplinary survey allowed a detailed characterization of shallow MDAC but was resource intensive. We recommend a low‐resource monitoring strategy to deliver cost‐effective and robust evidence for condition assessment and suggest further studies to contextualize future interpretations of change.

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Phylogenetic confirmation of the genusRobbea(Nematoda: Desmodoridae, Stilbonematinae) with the description of three new species

Cited by 9 publications

References 41 publications

Closely coupled evolutionary history of ecto‐ and endosymbionts from two distantly related animal phyla

Closely coupled evolutionary history of ecto‐ and endosymbionts from two distantly related animal phyla

Phylogenetic relationships within the superfamily Desmodoroidea (Nematoda: Desmodorida), with descriptions of two new and one known species

Monitoring shallow methane‐derived authigenic carbonate: Insights from a UK Marine Protected Area

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