Phylogenetic constrains on mycorrhizal specificity in eight Dendrobium (Orchidaceae) species

Xing, Xiaoke; Ma, Xiu-Wen; Men, Jinxin; Chen, Yanhong; Guo, Shun‐Xing

doi:10.1007/s11427-017-9020-1

Cited by 35 publications

(37 citation statements)

References 49 publications

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“…Because the three life forms represent largely different environments, they may select for different mycorrhizal fungi. Our results showed that members of the Tulasnellaceae were the most dominant fungi in the three life forms, supporting previous findings that Tulasnellaceae symbionts are ubiquitous in terrestrial orchids worldwide (Jacquemyn et al 2017) as well as in some epiphytic orchids (Kartzinel et al 2013, Xing et al 2017. Besides members of the Tulasnellaceae, other rhizoctonia fungi of the Ceratobasidiaceae and Sebacinales (Dearnaley et al 2012) were observed, particularly in terrestrial and epiphytic orchids, but not in the lithophytic species, which almost exclusively associated with fungi from the Tulasnellaceae.…”

Section: Host Specificity and Selectivity In Terrestrial Epiphytic Asupporting

confidence: 89%

“…Ninety-six clones were randomly selected from each library and sequenced using the M13 forward primer. Our previous studies have shown that this was a large enough clonal pool for assessing total species diversity and sequencing completeness (Xing et al 2015(Xing et al , 2017. All clones were sequenced by GENEWIZ Inc., Beijing, China.…”

Section: Assessment Of Mycorrhizal Communitiesmentioning

confidence: 99%

“…Previous research has shown that in several orchid genera closely related species associated with more similar fungal communities (e.g. Cypripedium (Shefferson et al 2007), Goodyera (Shefferson et al 2010), Orchis (Jacquemyn et al 2011), Dendrobium (Xing et al 2017)), suggesting that phylogenetic constraints may influence the mycorrhizal community an orchid associates with. However, in this research no phylogenetic signal on the overall network structure was detected.…”

Section: Phylogenetic Constraintsmentioning

confidence: 99%

“…In this case, variation in local growth conditions and the patchy distribution of compatible fungi may explain modularity (Jacquemyn et al 2012. In case host selectivity and specialization are also phylogenetically conserved (Shefferson et al 2005, Xing et al 2017, the network of interactions and modularity should also show a significant phylogenetic signal (Jacquemyn et al 2011).…”

Section: Introductionmentioning

confidence: 99%

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The impact of life form on the architecture of orchid mycorrhizal networks in tropical forest

Xing

Jacquemyn

Gai

et al. 2019

Oikos

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Understanding the processes that determine the architecture of interaction networks represents a major challenge in ecology and evolutionary biology. One of the most important interactions involving plants is the interaction between plants and mycorrhizal fungi. While there is a mounting body of research that has studied the architecture of plant–fungus interaction networks, less is known about the potential factors that drive network architecture. In this study, we described the architecture of the network of interactions between mycorrhizal fungi and 44 orchid species that represented different life forms and co‐occurred in tropical forest and assessed the relative importance of ecological, evolutionary and co‐evolutionary mechanisms determining network architecture. We found 87 different fungal operational taxonomic units (OTUs), most of which were members of the Tulasnellaceae. Most orchid species associated with multiple fungi simultaneously, indicating that extreme host selectivity was rare. However, an increasing specificity towards Tulasnellaceae fungal associates from terrestrial to epiphytic and lithophytic orchids was observed. The network of interactions showed an association pattern that was significantly modular (M = 0.7389, Mrandom = 0.6998) and nested (NODF = 5.53, p < 0.05). Terrestrial orchids had almost no links to modules containing epiphytic or lithophytic orchids, while modules containing epiphytic orchids also contained lithophytic orchids. Within each life form several modules were observed, suggesting that the processes that organize orchid–fungus interactions are independent of life form. The overall phylogenetic signal for both partners in the interaction network was very weak. Overall, these results indicate that tropical orchids associate with a wide number of mycorrhizal fungi and that ecological rather than phylogenetic constraints determine network architecture.

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Section: Host Specificity and Selectivity In Terrestrial Epiphytic Asupporting

confidence: 89%

Section: Assessment Of Mycorrhizal Communitiesmentioning

confidence: 99%

Section: Phylogenetic Constraintsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The impact of life form on the architecture of orchid mycorrhizal networks in tropical forest

Xing

Jacquemyn

Gai

et al. 2019

Oikos

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“…The occurrence and geographical variation in local abundance of fungal strains can be influenced by local habitat conditions (Pandey, Sharma, Taylor, & Yadon, ) and OMF community composition may therefore be the result of complex interactions between different factors, including extrinsic factors (habitat type, geographical site, soil characteristics, etc.) and intrinsic factors (genetic differentiation, phylogeny of host plants; Chen et al, ; McCormick & Jacquemyn, ; Swarts & Dixon, ; Xing, Ma, Men, Chen, & Guo, ). Previous research has, for example, shown that variation in local environmental factors such as soil moisture content, pH, nutrient conditions (especially soil carbon, nitrogen and phosphorus) can generate pronounced differences in mycorrhizal communities (Bunch, Cowden, Wurzburger, & Shefferson, ; Jacquemyn et al, ).…”

Section: Discussionmentioning

confidence: 99%

Similarity in mycorrhizal communities associating with two widespread terrestrial orchids decays with distance

Xing

Gao

Zhao

et al. 2019

Journal of Biogeography

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Aim Interactions with mycorrhizal fungi are increasingly recognized as an important factor underlying the distribution and abundance of orchid species. However, the geographical distribution of orchid mycorrhizal fungi (OMF) and how their communities vary over large geographical areas are less well understood. Because climatic and environmental similarity may decrease with geographical distance or because some OMF have limited dispersal capabilities, similarities in orchid mycorrhizal communities can be expected to decrease with increasing distances separating orchid populations. However, up till now empirical evidence is largely lacking. Location Eurasia. Taxa Gymnadenia conopsea (L.) R. Brown and Epipactis helleborine (L.) Crantz. Methods High‐throughput sequencing was used to perform a cross‐continental comparison of OMF that associate with two widespread Eurasian terrestrial orchids, Epipactis helleborine and Gymnadenia conopsea. Both phylogenetic and nonphylogenetic measures of community dissimilarity and their components were calculated and related to geographical distances using Mantel tests. Results Our results showed that in both orchid species similarity in mycorrhizal communities decreased significantly with geographical distance. Decomposing the contribution of spatial turnover and nestedness to overall dissimilarity showed that the observed dissimilarity was mainly the result of species replacement between regions, and not of species loss. Similarly, a strong relationship was observed between phylogenetic community dissimilarity and geographical distance. Decomposing PCD values into a nonphylogenetic and phylogenetic component showed that orchid populations located closely next to each other were likely to contain the same operational taxonomic units (OTUs), but that the non‐shared taxa came from different phylogenetic clades. Species indicator analyses showed that the majority of OMF OTUs were restricted to particular geographical areas. However, some OTUs occurred in both continents, indicating that some fungi have very wide distributions. Main conclusions Overall, these results demonstrate that orchid mycorrhizal communities differ substantially across large geographical areas, but that the distribution of orchids is not necessarily restricted by the distribution of particular OMF. Hence, widespread orchid species can be considered mycorrhizal generalists that are flexible in the OMF with which they associate across large geographical areas.

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Co‐occurring orchid species associated with different low‐abundance mycorrhizal fungi from the soil in a high‐diversity conservation area in Denmark

Hartvig,

Kosawang,

Rasmussen

et al. 2024

Ecology and Evolution

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Plant–fungal interactions are ubiquitous across ecosystems and contribute significantly to plant ecology and evolution. All orchids form obligate symbiotic relationships with specific fungi for germination and early growth, and the distribution of terrestrial orchid species has been linked to occurrence and abundance of specific orchid mycorrhizal fungi (OMF) in the soil. The availability of OMF can therefore be a habitat requirement that is relevant to consider when establishing management and conservation strategies for threatened orchid species, but knowledge on the spatial distribution of OMF in soil is limited. We here studied the mycorrhizal associations of three terrestrial orchid species (Anacamptis pyramidalis, Orchis purpurea and Platanthera chlorantha) found in a local orchid diversity hotspot in eastern Denmark, and investigated the abundance of the identified mycorrhizal fungi in the surrounding soil. We applied ITS metabarcoding to samples of orchid roots, rhizosphere soil and bulk soil collected at three localities, supplemented with standard barcoding of root samples with OMF specific primers, and detected 22 Operational Taxonomic Units (OTUs) putatively identified as OMF. The three orchid species displayed different patterns of OMF associations, supporting the theory that association with specific fungi constitutes part of an orchid's ecological niche allowing co‐occurrence of many species in orchid‐rich habitats. The identified mycorrhizal partners in the basidiomycete families Tulasnellaceae and Ceratobasidiaceae (Cantharallales) were detected in low abundance in rhizosphere soil, and appeared almost absent from bulk soil at the localities. This finding highlights our limited knowledge of the ecology and trophic mode of OMF outside orchid tissues, as well as challenges in the detection of specific OMF with standard methods. Potential implications for management and conservation strategies are discussed.

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Phylogenetic constrains on mycorrhizal specificity in eight Dendrobium (Orchidaceae) species

Cited by 35 publications

References 49 publications

The impact of life form on the architecture of orchid mycorrhizal networks in tropical forest

The impact of life form on the architecture of orchid mycorrhizal networks in tropical forest

Similarity in mycorrhizal communities associating with two widespread terrestrial orchids decays with distance

Co‐occurring orchid species associated with different low‐abundance mycorrhizal fungi from the soil in a high‐diversity conservation area in Denmark

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