Phylogenetic Distribution of Virulence Genes Among ESBL-producing Uropathogenic Escherichia coli Isolated from Long-term Hospitalized Patients

Zhao, Rongsheng; Shi, Jinfang; Shen, Yimin; Li, Yanmeng; Han, Qingkai; Zhang, Xianfeng; Gu, Guohao; Xu, Jie

doi:10.7860/jcdr/2015/13234.6157

Cited by 31 publications

(26 citation statements)

References 27 publications

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“…UPEC strains of Mexican origin are mainly associated with phylogenetic group B2 (López-Banda et al, 2014). Moreover, ESBLs-producing MDR-UPEC strains were mainly associated with phylogenetic groups B2, A, and D, whereas the XDR-UPEC strains were associated with A, B2, and D. The recently reported distribution of UPEC strains associated with these phylogenetic groups agrees with our data (Zhao et al, 2015). Commensal E. coli strains with the ability to cause community-acquired UTIs have been related to phylogenetic group B1, which was not identified in this study (Mosquito et al, 2015).…”

Section: Discussionsupporting

confidence: 92%

“…In this study, MDR and XDR-UPEC strains from pediatric patients at HIMFG were analyzed for their phylogeny, integrons, and virulence profile. These strains were mainly associated with phylogenetic groups, B2 and D as described other studies; however, they also have been associated with phylogenetic group A (Ejrnæs et al, 2011; Zhao et al, 2015). These UPEC strains could be considered community strains with predisposing factors that facilitate UTIs in immunocompromised patients, as previously reported (Derakhshandeh et al, 2015; Rodrigues et al, 2015).…”

Section: Discussionsupporting

confidence: 69%

“…The genes that encode adhesins (EcpA, FimH, PapG, and CsgA) and the protein related to iron uptake (siderophores) were widely distributed in MDR- and XDR-UPEC strains associated with two phylogenetic groups (B2 and D) and to a lesser extent with A. Recently, UPEC strains associated with phylogenetic group B2 and D showed a high presence of siderophores, autotransporters protease, and adhesion genes, while a low presence of genes encoding toxins was also observed (López-Banda et al, 2014; Yahiaoui et al, 2015; Zhao et al, 2015). In addition, the PapG II variant gene from MDR- and XDR-UPEC strains associated with phylogenetic groups D and B2 was the most prevalent compared to the papG I and papG III variant genes.…”

Section: Discussionmentioning

confidence: 99%

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Multidrug- and Extensively Drug-Resistant Uropathogenic Escherichia coli Clinical Strains: Phylogenetic Groups Widely Associated with Integrons Maintain High Genetic Diversity

et al. 2016

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In recent years, an increase of uropathogenic Escherichia coli (UPEC) strains with Multidrug-resistant (MDR) and Extensively Drug-resistant (XDR) profiles that complicate therapy for urinary tract infections (UTIs) has been observed and has directly impacted costs and extended hospital stays. The aim of this study was to determine MDR- and XDR-UPEC clinical strains, their virulence genes, their phylogenetic groups and to ascertain their relationship with integrons and genetic diversity. From a collection of 500 UPEC strains, 103 were selected with MDR and XDR characteristics. MDR-UPEC strains were mainly associated with phylogenetic groups D (54.87%) and B2 (39.02%) with a high percentage (≥70%) of several fimbrial genes (ecpA, fimH, csgA, and papGII), an iron uptake gene (chuA), and a toxin gene (hlyA). In addition, a moderate frequency (40–70%) of other genes (iutD, tosA, and bcsA) was observed. XDR-UPEC strains were predominantly associated with phylogenetic groups B2 (47.61%) and D (42.85%), which grouped with ≥80 virulence genes, including ecpA, fimH, csgA, papGII, iutD, and chuA. A moderate frequency (40–70%) of the tosA and hlyA genes was observed. The class 1 and 2 integrons that were identified in the MDR- and XDR-UPEC strains were associated with phylogenetic groups D, B2, and A, while the XDR-UPEC strains that were associated with phylogenetic groups B2, D, and A showed an extended-spectrum beta-lactamase (ESBL) phenotype. The modifying enzymes (aadA1, aadB, aacC, ant1, dfrA1, dfrA17, and aadA4) that were identified in the variable region of class 1 and 2 integrons from the MDR strains showed resistance to gentamycin (56.25 and 66.66%, respectively) and trimethoprim-sulfamethoxazole (84.61 and 66.66%, respectively). The MDR- and XDR-UPEC strains were distributed into seven clusters and were closely related to phylogenic groups B2 and D. The diversity analysis by PFGE showed 42.68% of clones of MDR-UPEC and no clonal association in the XDR-UPEC strains. In conclusion, phylogenetic groups including virulence genes are widely associated with two integron classes (1 and 2) in MDR- and XDR-UPEC strains.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 69%

Section: Discussionmentioning

confidence: 99%

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Multidrug- and Extensively Drug-Resistant Uropathogenic Escherichia coli Clinical Strains: Phylogenetic Groups Widely Associated with Integrons Maintain High Genetic Diversity

et al. 2016

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“…The prevalence of iutA and traT was high in E. coli isolates in China (75.8% for traT) [28] and India (68% for iutA) [29], which is similar to this study, but 23% of isolates from India were hlyA and cnf1 positive. The high rate of drug resistance and the presence of virulence factors in E. coli isolates increase the danger of fatal infections with no response to therapy.…”

Section: Pcr Detection Of E Coli Virulence Genessupporting

confidence: 75%

The antibiotic resistance and virulence factors of Escherichia coli of serogroup O1 isolated from Tehran, Iran

Eslami¹,

Peerayeh²,

Abadi³

et al. 2017

APJTD

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“…Ten years ago a plasmid-mediated enzymatic inactivation mechanism gene, aac(6′)Ib-cr, which could contribute to the development of quinolone resistance, was identified (Robicsek et al, 2006b). The aac(6′)Ib-cr gene encodes for a bifunctional aminoglycoside 6′-N-acetyltransferase enzyme, which has been associated with resistance to kanamycin, as well as, ciprofloxacin in several bacterial species, including E. coli (Cambau and Guillard, 2012;Szmolka et al, 2011;Zhao et al, 2015). The PMQR genes, oqxAB and qepA encode efflux pumps and result in resistance.…”

Section: Quinolonesmentioning

confidence: 99%

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

Awawdeh¹,

Turni²,

Henning³

et al.

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Avian pathogenic Escherichia coli (APEC) is the causative agent of avian colibacillosis, a localised or systemic infection resulting in clinical diseases such as colisepticemia, chronic respiratory disease and swollen-head syndrome. Globally, avian colibacillosis is the leading cause of morbidity and mortality in poultry, and it has been associated with massive economic losses and welfare problems.This organism is of public health significance as APEC is communicable to humans. The diagnosis of avian colibacillosis relies on clinical signs, typical pathological lesions and culture of E. coli from affected tissue(s). Antimicrobial therapy is often used both for treatment and control. Previous overseas studies have characterised APEC and identified virulence genes (VGs) that can be used as molecular markers for the identification of APEC. Little is known about APEC in broiler chickens in Australia.The aim of this thesis was to gain a better understanding of the epidemiology of APEC in Australian broiler flocks and how factors including presence of VGs and phylogenetic group can improve the identification of this pathotype in Australia. Firstly, three faecal DNA extraction methods were evaluated. Faeces were collected from healthy chickens and chickens with colibacillosis from commercial broiler farms in South East Queensland (SEQ). The extracted DNA was screened by a pentaplex-PCR for five APEC-associated VGs (iroN, iutA, iss, hlyF and ompT). DNA extracted from E. coli isolates cultured from the cloaca and organs of the birds were screened using the same PCR.Repeated bead beating plus column elution was the preferred DNA extraction method, as it yielded good PCR quality and adequate quantity DNA. However, identifying APEC by direct detection of the five VGs from the faecal material was not feasible as all of these genes were also detected in all of the birds. However, the VGs were more commonly detected in E. coli isolates cultured from birds with colibacillosis.A cross-sectional study was performed to estimate APEC farm-level prevalence in healthy broiler chickens in SEQ and to identify potential risk factors associated with the carriage of APEC. At the farm-level, all of the 40 farms sampled were positive for APEC, that is at least one bird per farm carried APEC, while the within-farm prevalence was 63% (95% Confidence Interval: 55.8, 70.2).Higher APEC within-farm bird-level prevalence was significantly associated with the usage of well water as a source of drinking water, failure to disinfect the water line after each flock, farm visitors not showering before entering the shed, distances greater than 20 metres between the car park and the poultry shed and the presence of wild birds within 50 metres of the shed. Chlorinating the drinking water combined with automatic water filtration reduced within-farm bird-level APEC prevalence. Page | 3Therefore, based on the results concluded from the multivariable model, improving biosecurity and water treatments might reduce APEC prevalence, decrease the risk of co...

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Phylogenetic Distribution of Virulence Genes Among ESBL-producing Uropathogenic Escherichia coli Isolated from Long-term Hospitalized Patients

Cited by 31 publications

References 27 publications

Multidrug- and Extensively Drug-Resistant Uropathogenic Escherichia coli Clinical Strains: Phylogenetic Groups Widely Associated with Integrons Maintain High Genetic Diversity

Multidrug- and Extensively Drug-Resistant Uropathogenic Escherichia coli Clinical Strains: Phylogenetic Groups Widely Associated with Integrons Maintain High Genetic Diversity

The antibiotic resistance and virulence factors of Escherichia coli of serogroup O1 isolated from Tehran, Iran

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

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