Phylogenetic divergence between Rickettsia amblyommatis strains from Argentina

Sébastian, Patrick; Tarragona, Evelina Luisa; Bottero, María Noelia Saracho; Nava, Stefano

doi:10.1016/j.cimid.2020.101418

Cited by 6 publications

(5 citation statements)

References 56 publications

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“…Since A. americanum is the most frequent carrier of R. amblyommatis , movement by deer may have led to the geographic expansion of R. amblyommatis . Ticks are considered both the reservoir and the vector for R. amblyommatis which causes quicker and further spread of this spotted fever species as it does not have to depend on hosts to proliferate [ 27 , 55 , 131 , 189 , 190 ] and, indeed, the geography of tick-borne rickettsioses such as R. amblyommatis , may be mainly based on the distribution of the vector tick species [ 131 , 190 ]. Another aspect that could be helping fuel this spread could be Amblyomma spp.’s generalist tendencies when finding a host, increasing their likelihood of survival by finding a host quickly and moving to the next life stage [ 191 ].…”

Section: Distribution and Spread Of Rickettsia Amblyommatismentioning

confidence: 99%

“…Past studies have hypothesized that various strains of R. amblyommatis could be genetically independent, with differences evolving based on the tick species and geographic location [ 99 , 190 ]. Dolz et al [ 147 ] found that Costa Rican strains of R. amblyommatis showed intraspecific variation and found high associations with strains from some South American countries.…”

Section: Phylogenetic Analysismentioning

confidence: 99%

“…We created two trees, one that focused on the gene targeting the outer membrane protein A ( ompA ) and a second targeting outer membrane protein B ( ompB ). Sequences focusing on these two gene regions were selected as they have been noted to be polymorphic, with ompB being suitable for analyzing intraspecific differences in R. amblyommatis [ 190 ]. The methods involved in the creation of the phylogenetic trees can be seen in the Materials and Methods section.…”

Section: Phylogenetic Analysismentioning

confidence: 99%

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Rickettsia amblyommatis in Ticks: A Review of Distribution, Pathogenicity, and Diversity

et al. 2023

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Rickettsia amblyommatis is a potentially pathogenic species of Rickettsia within the spotted fever group vectored by ticks. While many studies have been published on this species, there is debate over its pathogenicity and the inhibitory role it plays in diagnosing illnesses caused by other spotted fever group Rickettsia species. Many publications have recorded the high infection prevalence of R. amblyommatis in tick populations at a global scale. While this species is rather ubiquitous, questions remain over the epidemiological importance of this possible human pathogen. With tick-borne diseases on the rise, understanding the exact role that R. amblyommatis plays as a pathogen and inhibitor of infection relative to other tick-borne pathogens will help public health efforts. The goal of this review was to compile the known literature on R. amblyommatis, review what we know about its geographic distribution, tick vectors, and pathogenicity, assess relatedness between various international strains from ticks by phylogenetic analysis and draw conclusions regarding future research needed.

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Section: Distribution and Spread Of Rickettsia Amblyommatismentioning

confidence: 99%

Section: Phylogenetic Analysismentioning

confidence: 99%

Section: Phylogenetic Analysismentioning

confidence: 99%

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Rickettsia amblyommatis in Ticks: A Review of Distribution, Pathogenicity, and Diversity

et al. 2023

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“…However, here we provide new data on R. amblyommatis infecting A. romitii and A. nodosum ticks. Considering the possibility of the genetic variability between the different strains of R. amblyommatis in South American (Sebastian et al, 2020), it is evident the importance of new data encompassing infection by this bacterium in two species of the genus Amblyomma, also contributing to a better understanding of ecological relationships involving ticks and agents transmitted by them in a wild environment. Despite the numerous reports of infection in ticks, the pathogenicity of R. amblyommatis to humans is still unknown.…”

Section: Discussionmentioning

confidence: 99%

Diversity of rickettsiae in ticks (Acari: Ixodidae) collected from wild vertebrates in part of the Amazon, Cerrado, and Pantanal biomes in Brazil

Prati,

Maia,

Martins

et al. 2023

Rev. Bras. Parasitol. Vet.

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Ticks parasitizing 102 wild animals in the states of Mato Grosso and Goiás, Brazil were collected between 2015 and 2018. A total of 2338 ticks (865 males, 541 females, 823 nymphs, and 109 larvae) belonging to four genera (Amblyomma, Dermacentor, Haemaphysalis, and Rhipicephalus) and at least 21 species were identified. DNA extraction and a molecular survey for rickettsial agents were performed on 650 ticks. The results revealed parasitism by the following species: Rickettsia amblyommatis in Amblyomma cajennense s.s., A. cajennense s.l., Amblyomma coelebs, Amblyomma humerale, Amblyomma longirostre, Amblyomma nodosum, Amblyomma scalpturatum, Amblyomma sculptum, and Amblyomma romitii; Rickettsia parkeri in Amblyomma nodosum, Amblyomma ovale, Amblyomma scalpturatum, and Amblyomma triste; Rickettsia rhipicephali in Haemaphysalis juxtakochi; Rickettsia sp. in A. cajennense s.s., A. nodosum, and A. sculptum, and lastly, ‘Candidatus Rickettsia andeanae’ in Amblyomma parvum and Rhipicephalus microplus. This study expands the body of knowledge about tick parasitism among wild animals, including new data concerning tick-host associations, and provides information about the epidemiology of tick-borne pathogens in the Center-West region of Brazil.

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“…Rickettsia bellii and Rickettsia amblyommatis have been found in numerous tick species [ 8 – 10 ]. These rickettsial species have been discovered in almost every tick species in the Neotropics, revealing a wide phylogenetic diversity of ticks and molecular differentiation of R. amblyommatis strains [ 11 ]. Although R. amblyommatis is widely distributed in the Neotropical region, it has not yet been determined whether it is a pathogen for vertebrates.…”

Section: Introductionmentioning

confidence: 99%

First isolation of Rickettsia amblyommatis from Amblyomma mixtum in Colombia

Chaparro-Gutiérrez,

Acevedo-Gutiérrez,

Mendell

et al. 2023

Parasites Vectors

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Background Rickettsiae are obligate intracellular Gram-negative bacteria that are the causative agent of rickettsioses and are spread to vertebrate hosts by arthropods. There are no previous reports of isolation of Rickettsia amblyommatis for Colombia. Methods A convenience sampling was executed in three departments in Colombia for direct collection of adult ticks on domestic animals or over vegetation. Ticks were screened for the presence of Rickettsia spp. by real-time polymerase chain reaction (qPCR) amplifying the citrate synthase gene (gltA), and the positive sample was processed for isolation and further molecular characterization by conventional PCR. The absolute and relative frequencies were calculated for several tick species variables. All products from conventional PCR were further purified and sequenced by the Sanger technique. Representative sequences of 18 Rickettsia species were downloaded from GenBank. Consensus phylogenetic trees were constructed for the gltA, ompB, ompA, and htrA genes with 1000 replicates, calculating bootstrap values through the maximum likelihood method and the generalized time reversible substitution model in the MEGA 7.0 software program. Results One female Amblyomma mixtum collected on vegetation was amplified by qPCR (gltA), indicating a frequency of 1.6% (1/61) for Rickettsia spp. infection. Sequence analysis of a rickettsial isolate from this tick in BLASTn showed 100% identity with gltA (340 base pairs [bp]), 99.87% for ompB (782 bp), 98.99% for htrA (497 bp), and 100% for ompA (488 bp) to R. amblyommatis. Concatenated phylogenetic analysis confirmed these findings indicating that the isolate is grouped with other sequences of Amblyomma cajennense complex from Panama and Brazil within the R. amblyommatis clade. Conclusions This paper describes the isolation and early molecular identification of a R. amblyommatis strain from A. mixtum in Colombia. Graphical Abstract

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Phylogenetic divergence between Rickettsia amblyommatis strains from Argentina

Cited by 6 publications

References 56 publications

Rickettsia amblyommatis in Ticks: A Review of Distribution, Pathogenicity, and Diversity

Rickettsia amblyommatis in Ticks: A Review of Distribution, Pathogenicity, and Diversity

Diversity of rickettsiae in ticks (Acari: Ixodidae) collected from wild vertebrates in part of the Amazon, Cerrado, and Pantanal biomes in Brazil

First isolation of Rickettsia amblyommatis from Amblyomma mixtum in Colombia

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