Phylogenetic Diversity and Antifungal Activity of Endophytic Fungi Associated with <i>Tephrosia purpurea</i>

Luo, Ze-Ping; Lin, Haiyan; Ding, Wenbing; He, Hualiang; Li, Youzhi

doi:10.5941/myco.2015.43.4.435

Cited by 16 publications

(5 citation statements)

References 35 publications

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“…The antifungal hit rates observed for our isolates were within the range of values reported for other studies with endophytic strains from Mediterranean areas 41 , 44 . Regarding the cytotoxic hit rates (19.2%), no previous data was available related with other antitumoral screenings performed with endophytes from arid areas.…”

Section: Discussionsupporting

confidence: 89%

Fungal endophytes from arid areas of Andalusia: high potential sources for antifungal and antitumoral agents

González-Menéndez

Crespo

Pedro

et al. 2018

Sci Rep

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Native plant communities from arid areas present distinctive characteristics to survive in extreme conditions. The large number of poorly studied endemic plants represents a unique potential source for the discovery of novel fungal symbionts as well as host-specific endophytes not yet described. The addition of adsorptive polymeric resins in fungal fermentations has been seen to promote the production of new secondary metabolites and is a tool used consistently to generate new compounds with potential biological activities. A total of 349 fungal strains isolated from 63 selected plant species from arid ecosystems located in the southeast of the Iberian Peninsula, were characterized morphologically as well as based on their ITS/28S ribosomal gene sequences. The fungal community isolated was distributed among 19 orders including Basidiomycetes and Ascomycetes, being Pleosporales the most abundant order. In total, 107 different genera were identified being Neocamarosporium the genus most frequently isolated from these plants, followed by Preussia and Alternaria. Strains were grown in four different media in presence and absence of selected resins to promote chemical diversity generation of new secondary metabolites. Fermentation extracts were evaluated, looking for new antifungal activities against plant and human fungal pathogens, as well as, cytotoxic activities against the human liver cancer cell line HepG2. From the 349 isolates tested, 126 (36%) exhibited significant bioactivities including 58 strains with exclusive antifungal properties and 33 strains with exclusive activity against the HepG2 hepatocellular carcinoma cell line. After LCMS analysis, 68 known bioactive secondary metabolites could be identified as produced by 96 strains, and 12 likely unknown compounds were found in a subset of 14 fungal endophytes. The chemical profiles of the differential expression of induced activities were compared. As proof of concept, ten active secondary metabolites only produced in the presence of resins were purified and identified. The structures of three of these compounds were new and herein are elucidated.

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Section: Discussionsupporting

confidence: 89%

Fungal endophytes from arid areas of Andalusia: high potential sources for antifungal and antitumoral agents

González-Menéndez

Crespo

Pedro

et al. 2018

Sci Rep

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“…Zheng et al. [ 31 ] proposed that the colonization rate of endophytic fungi is generally significantly higher in the stems than in the leaves, but we found the opposite; nevertheless, the results of several previous studies were similar to ones obtained in the current study [ 11 , 35 ]. The possible reasons for these discrepancies are different organ structure, chemical composition, and even the period length of organs stay remaining in the tree, possibly leading to plant intracellular substance composition variance [ 27 , 36 , 37 ].…”

Section: Discussionsupporting

confidence: 77%

Diversity and Antifungal Activity of Endophytic Fungi Associated with Camellia oleifera

Wu²,

He³

et al. 2018

Mycobiology

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Endophytic fungi strains (n = 81) were isolated from the leaves, barks, and fruits of Camellia oleifera from Hunan province (China) to delineate their species composition and potential as biological control agents of C. oleifera anthracnose. The fungi were identified by morphological and phylogenetic analyses. Fungal colonization rates of the leaves, barks, and fruits were 58.02, 27.16, and 14.81%, respectively. The isolates were identified as 14 genera, belonging to two subdivisions, Deuteromycotina and Ascomycotina; 87.65% of all isolates belonged to Deuteromycotina. The dominant species, occurring with a high relative frequency, were Pestalotiopsis sp. (14.81%), Penicillium sp. (14.81%), and Fusarium sp. (12.35%). The Simpson’s and Shannon’s diversity indices revealed the highest species diversity in the leaves, followed by the barks and fruits. The similarity index for the leaves versus barks comparison was the highest, indicating that the number of endophytic fungal species shared by the leaves and barks was higher than barks and fruits or leaves and fruits. Based on the results of dual culture experiments, only five strains exhibited antifungal activity against C. oleifera anthracnose pathogen, with isolate ty-64 (Oidium sp.) generating the broadest inhibition zones. Our results indicate that the endophytes associated with C. oleifera could be employed as natural agents controlling C. oleifera anthracnose.

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“…The most frequent endophytic fungi of Tephrosia purpurea belonged to the genera Nigrospora (14.7%) and Penicillium (9.8%) (Luo et al, 2015). The genus Phomopsis represented 46% of the isolates obtained from Picea sitchensis (Carroll & Carroll, 1978).…”

Section: Discussionmentioning

confidence: 99%

Endophytic fungi of Brunfelsia uniflora: isolation, cryopreservation, and determination of enzymatic and antioxidant activity

Marsola

Jorge

Meniqueti

et al. 2022

World J Microbiol Biotechnol

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Brunfelsia uniflora (Pohl.) D. Don (Solanaceae), commonly known as manacá-de-cheiro, is widely distributed in Brazil and used by local indigenous peoples as an antirheumatic, antisyphilitic, depurative, emetic, vermifuge, and purgative agent. Several studies have examined the biological activities and phytochemical profile of Brunfelsia; however, few have focused on the diversity of endophytic microorganisms that colonize members of the genus. This study aimed to isolate and cryopreserve endophytic fungi from B. uniflora and determine their cellulase, laccase, and antioxidant activities.Endophytic fungi were isolated from B. uniflora stems, cultured on wheat grains, immersed in a 150 g•L -1 aqueous sucrose solution, and cryopreserved at −80°C for 1 and 6 months. Cellulase activity was determined by a qualitative test using carboxymethylcellulose medium and laccase activity by a quantitative test based on the oxidation of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate). Prior to antioxidant activity assays, fungi were grown in malt extract broth for production of mycelial biomass. A methanolic extract was prepared for evaluation of DPPH• scavenging activity, FRAP activity, and total phenolic content. A total of 46 endophytic fungal isolates were obtained from B. uniflora stems and classified into 24 groups according to morphological similarities. B. uniflora was shown to harbor a great diversity of endophytic fungi. Mycelial viability was observed after 1 and 6 months of cryopreservation at −80 °C. Fungi exhibited cellulase and laccase activities. Isolate CE23 had the highest laccase activity after 7 days of cultivation. Twelve isolates were found to have low total phenolic contents and DPPH• and FRAP activities.

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Phylogenetic Diversity and Antifungal Activity of Endophytic Fungi Associated with Tephrosia purpurea

Cited by 16 publications

References 35 publications

Fungal endophytes from arid areas of Andalusia: high potential sources for antifungal and antitumoral agents

Fungal endophytes from arid areas of Andalusia: high potential sources for antifungal and antitumoral agents

Diversity and Antifungal Activity of Endophytic Fungi Associated with Camellia oleifera

Endophytic fungi of Brunfelsia uniflora: isolation, cryopreservation, and determination of enzymatic and antioxidant activity

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