Phylogenetic Diversity and Symbiotic Effectiveness of Root-Nodulating Bacteria Associated with Cowpea in the South-West Area of Japan

Abstract

In Table3, TSC1 isolate was shown nodulating the four cowpea cultivars with an acetylene-reducing activity. However, by further purification of the TSC1 culture on AIE agar plates, it is likely that it was contaminated with compatible bradyrhizobia which certainly had caused the nodulation when TSC1 was inoculated to cowpea. In addition, repetitive inoculations tests of this purified isolate (TSC1) to cowpea have indicated that Ralstonia sp. TSC1 has no nodulation and no nitrogen fixation capability. As a result, we decide to delete the previous Table 3, and edited the text as follows:Page 110, line 13 to 19 of left column (Cross-inoculation with TSC1………..Dan IIa, Tv-7778): Cross-inoculation with TSC1 (data not shown) indicated that this strain didn't react with any of the four host cultivars and had no nodulation and no nitrogen fixation capability.Page 111, line 17 to 19 of left column (Further molecular analysis……..symbiotic characteristic): Further analysis should be carried out on the novel species isolated from cowpea (Ralstonia sp. TSC1) to better understand its features.The paper has scientific significance in that it provides valuable information on the diversity and phylogeny of cowpea nodulating-bacteria in the West-South area of Japan. Furthermore, among the isolates described in the paper, strains with high nitrogen-fixing potential were identified.

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